LOCUS SOYLEA 2152 bp DNA linear PLN 27-APR-1993
DEFINITION Soybean lectin (Le1) gene, complete cds.
ACCESSION K00821 M30884
VERSION K00821.1
KEYWORDS lectin; transposon.
SOURCE Glycine max (soybean)
ORGANISM Glycine max
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50
kb inversion clade; NPAAA clade; indigoferoid/millettioid clade;
Phaseoleae; Glycine; Glycine subgen. Soja.
REFERENCE 1 (bases 1 to 2152)
AUTHORS Vodkin,L.O., Rhodes,P.R. and Goldberg,R.B.
TITLE cA lectin gene insertion has the structural features of a
transposable element
JOURNAL Cell 34 (3), 1023-1031 (1983)
PUBMED 6313203
REFERENCE 2 (bases 719 to 819)
AUTHORS Jofuku,K.D., Okamuro,J.K. and Goldberg,R.B.
TITLE Interaction of an embryo DNA binding protein with a soybean lectin
gene upstream region
JOURNAL Nature 328 (6132), 734-737 (1987)
PUBMED 3614381
COMMENT Original source text: Soybean seed (cv. forrest and sooty) DNA.
[1] sequenced Le1 and a naturally occurring allelic variant of Le1
that differs only by six single-base mutations and the presence of
a 3.4 kb transposon-like insertion in the cds. The allelic
sequencing covered nucleotides 769-2152.
FEATURES Location/Qualifiers
source 1..2152
/organism="Glycine max"
/mol_type="genomic DNA"
/db_xref="taxon:3847"
variation 794
/note="a in Le1; c in allele"
variation 909
/note="t in Le1; a in allele"
mRNA 939..1952
/note="Le1 mRNA (alt.)"
mRNA 939..1921
/note="Le1 mRNA (alt.)"
CDS 969..1826
/note="lectin prepeptide"
/codon_start=1
/protein_id="AAA33983.1"
/translation="MATSKLKTQNVVVSLSLTLTLVLVLLTSKANSAETVSFSWNKFV
PKQPNMILQGDAIVTSSGKLQLNKVDENGTPKPSSLGRALYSTPIHIWDKETGSVASF
AASFNFTFYAPDTKRLADGLAFFLAPIDTKPQTHAGYLGLFNENESGDQVVAVEFDTF
RNSWDPPNPHIGINVNSIRSIKTTSWDLANNKVAKVLITYDASTSLLVASLVYPSQRT
SNILSDVVDLKTSLPEWVRIGFSAATGLDIPGESHDVLSWSFASNLPHASSNIDPLDL
TSFVLHEAI"
sig_peptide 969..1064
/note="lectin signal peptide"
mat_peptide 1065..1823
/product="lectin mature peptide"
variation 1066
/note="c in Le1; t in allele"
variation 1229
/note="c in Le1; g in allele"
variation 1565..1567
/note="cta in Le1; cta...3.4 kb...cta in allele"
variation 1667
/note="c in Le1; t in allele"
variation 2035
/note="g in Le1; c in allele"
BASE COUNT 689 a 417 c 341 g 703 t
ORIGIN 416 bp upstream of HpaI site.
1 caatgccatc gtatcgtgtc acaatggaat acagcaatga acaaatgcta tcctcttgag
61 aaaagtgaaa tgcagcagca gcagcagact agagtgctac aaatgcttat cctcttgaga
121 aaagtgaaat gcagcggcag cagacctgag tgctatatac aattagacac agggtctatt
181 aattgaaatt gtcttattat taaatatttc gttttatatt aattttttaa attttaatta
241 aatttatata tattatattt aagacagata tatttatttg tgattataaa tgtgtcactt
301 tttcttttag tccatgtatt cttctatttt ttcaatttaa ctttttattt ttatttttaa
361 gtcactctga tcaagaaaac attgttgaca taaaactatt aacataaaat tatgttaaca
421 tgtgataaca tcatatttta ctaatataac gtcgcatttt aacgtttttt taacaaatat
481 cgactgtaag agtaaaaatg aaatgtttga aaaggttaat tgcatactaa ctattttttt
541 tcctataagt aatctttttt gggatcannt gtatatcatt gagatacgat attaaatatg
601 ggtacctttt cacaaaacct acccttgtta gtcaaaccac acataagaga ggatggattt
661 aaaccagtca gcaccgtaag tatatagtga agaaggctga taacacactc tattattgtt
721 agtacgtacg tatttccttt tttgtttagt ttttgaattt aattaattaa aatatatatg
781 ctaacaacat taaattttaa atttacgtct aattatatat tgtgatgtat aataaattgt
841 caacctttaa aaattataaa agaaatatta attttgataa acaacttttg aaaagtaccc
901 aataatgcta gtataaatag gggcatgact ccccatgcat cacagtgcaa tttagctgaa
961 gcaaagcaat ggctacttca aagttgaaaa cccagaatgt ggttgtatct ctctccctaa
1021 ccttaacctt ggtactggtg ctactgacca gcaaggcaaa ctcagcggaa actgtttctt
1081 tcagctggaa caagttcgtg ccgaagcaac caaacatgat cctccaagga gacgctattg
1141 tgacctcctc gggaaagtta caactcaata aggttgacga aaacggcacc ccaaaaccct
1201 cgtctcttgg tcgcgccctc tactccaccc ccatccacat ttgggacaaa gaaaccggta
1261 gcgttgccag cttcgccgct tccttcaact tcaccttcta tgcccctgac acaaaaaggc
1321 ttgcagatgg gcttgccttc tttctcgcac caattgacac taagccacaa acacatgcag
1381 gttatcttgg tcttttcaac gaaaacgagt ctggtgatca agtcgtcgct gttgagtttg
1441 acactttccg gaactcttgg gatccaccaa atccacacat cggaattaac gtcaattcta
1501 tcagatccat caaaacgacg tcttgggatt tggccaacaa taaagtagcc aaggttctca
1561 ttacctatga tgcctccacc agcctcttgg ttgcttcttt ggtctaccct tcacagagaa
1621 ccagcaatat cctctccgat gtggtcgatt tgaagacttc tcttcccgag tgggtgagga
1681 tagggttctc tgctgccacg ggactcgaca tacctgggga atcgcatgac gtgctttctt
1741 ggtcttttgc ttccaatttg ccacacgcta gcagtaacat tgatcctttg gatcttacaa
1801 gctttgtgtt gcatgaggcc atctaaatgt gacagatcga aggaagaaag tgtaataaga
1861 cgactctcac tactcgatcg ctagtgattg tcattgttat atataataat gttatctttc
1921 acaacttatc gtaatgcatt gtgaaactat aacacattta atcctacttg tcatatgata
1981 acactctccc catttaaaac tcttgtcaat ttaaagatat aagattcttt aaatgattaa
2041 aaaaaatata ttataaattc aatcactcct actaataaat tattaattaa tatttattga
2101 ttaaaaaaat acttatacta atttagtctg aatagaataa ttagattcta ga
//