LOCUS JX560328 3123 bp DNA circular SYN 02-OCT-2014
DEFINITION Cloning vector pSEVA231, complete sequence.
ACCESSION JX560328
VERSION JX560328.1
KEYWORDS .
SOURCE Cloning vector pSEVA231
ORGANISM Cloning vector pSEVA231
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3123)
AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B.,
Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D.,
Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de
Lorenzo,V.
TITLE The Standard European Vector Architecture (SEVA): a coherent
platform for analysis and deployment of complex prokaryotic
phenotypes
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3123)
AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B.,
Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D.,
Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de
Lorenzo,V.
TITLE Direct Submission
JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de
Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid,
Madrid 28049, Spain
COMMENT ##Assembly-Data-START##
Assembly Method :: ApE v. v2.0.40
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..3123
/organism="Cloning vector pSEVA231"
/mol_type="other DNA"
/db_xref="taxon:1246706"
primer_bind 9..32
/note="R24"
primer_bind complement(121..144)
/note="F24"
regulatory 151..253
/regulatory_class="terminator"
/note="T0"
gene 381..1196
/gene="neo"
CDS 381..1196
/gene="neo"
/codon_start=1
/transl_table=11
/product="neomycin phosphotransferase"
/protein_id="AFV59774.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
misc_feature 1228..1473
/note="oriT"
gene complement(1482..2144)
/gene="rep"
CDS complement(1482..2144)
/gene="rep"
/codon_start=1
/transl_table=11
/product="replication protein"
/protein_id="AFV59775.1"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
regulatory 3018..3122
/regulatory_class="terminator"
/note="T1"
BASE COUNT 617 a 871 c 929 g 706 t
ORIGIN
1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
241 ggtgagaatc caggggtccc caataattac gatttaaatt tgtgtctcaa aatctctgat
301 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
361 acagtaatac aaggggtgtt atgagccata ttcagcgtga aacgagctgt agccgtccgc
421 gtctgaacag caacatggat gcggatctgt atggctataa atgggcgcgt gataacgtgg
481 gtcagagcgg cgcgaccatt tatcgtctgt atggcaaacc ggatgcgccg gaactgtttc
541 tgaaacatgg caaaggcagc gtggcgaacg atgtgaccga tgaaatggtg cgtctgaact
601 ggctgaccga atttatgccg ctgccgacca ttaaacattt tattcgcacc ccggatgatg
661 cgtggctgct gaccaccgcg attccgggca aaaccgcgtt tcaggtgctg gaagaatatc
721 cggatagcgg cgaaaacatt gtggatgcgc tggccgtgtt tctgcgtcgt ctgcatagca
781 ttccggtgtg caactgcccg tttaacagcg atcgtgtgtt tcgtctggcc caggcgcaga
841 gccgtatgaa caacggcctg gtggatgcga gcgattttga tgatgaacgt aacggctggc
901 cggtggaaca ggtgtggaaa gaaatgcata aactgctgcc gtttagcccg gatagcgtgg
961 tgacccacgg cgattttagc ctggataacc tgattttcga tgaaggcaaa ctgattggct
1021 gcattgatgt gggccgtgtg ggcattgcgg atcgttatca ggatctggcc attctgtgga
1081 actgcctggg cgaatttagc ccgagcctgc aaaaacgtct gtttcagaaa tatggcattg
1141 ataatccgga tatgaacaaa ctgcaatttc atctgatgct ggatgaattt ttctaataat
1201 taattggacc gcggtccgcg cgttgtcctt ttccgctgca taaccctgct tcggggtcat
1261 tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag
1321 ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt
1381 aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct
1441 caacgggaat cctgctctgc gaggctggcc gtaggccggc cctaccggcg cggcagcgtt
1501 acccgtgtcg gcggctccaa cggctcgcca tcgtccagaa aacacggctc atcgggcatc
1561 ggcaggcgct gctgcccgcg ccgttcccat tcctccgttt cggtcaaggc tggcaggtct
1621 ggttccatgc ccggaatgcc gggctggctg ggcggctcct cgccggggcc ggtcggtagt
1681 tgctgctcgc ccggatacag ggtcgggatg cggcgcaggt cgccatgccc caacagcgat
1741 tcgtcctggt cgtcgtgatc aaccaccacg gcggcactga acaccgacag gcgcaactgg
1801 tcgcggggct ggccccacgc cacgcggtca ttgaccacgt aggccgacac ggtgccgggg
1861 ccgttgagct tcacgacgga gatccagcgc tcggccacca agtccttgac tgcgtattgg
1921 accgtccgca aagaacgtcc gatgagcttg gaaagtgtct tctggctgac caccacggcg
1981 ttctggtggc ccatctgcgc cacgaggtga tgcagcagca ttgccgccgt gggtttcctc
2041 gcaataagcc cggcccacgc ctcatgcgct ttgcgttccg tttgcaccca gtgaccgggc
2101 ttgttcttgg cttgaatgcc gatttctctg gactgcgtgg ccatgcttat ctccatgcgg
2161 taggggtgcc gcacggttgc ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga
2221 caacaattat gcgttgcgta aaagtggcag tcaattacag attttcttta acctacgcaa
2281 tgagctattg cggggggtgc cgcaatgagc tgttgcgtac cccccttttt taagttgttg
2341 atttttaagt ctttcgcatt tcgccctata tctagttctt tggtgcccaa agaagggcac
2401 ccctgcgggg ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc
2461 cggggcttgt tgatcgactg cgcggccttc ggccttgccc aaggtggcgc tgcccccttg
2521 gaacccccgc actcgccgcc gtgaggctcg gggggcaggc gggcgggctt cgcccttcga
2581 ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag ccgctgcgcg
2641 gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc aaccggcaag cgaagcgcgc
2701 aggccgcagg ccggaggctt ttccccagag aaaattaaaa aaattgatgg ggcaaggccg
2761 caggccgcgc agttggagcc ggtgggtatg tggtcgaagg ctgggtagcc ggtgggcaat
2821 ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc agcttgtcca gcagggttgt
2881 ccacgggccg agcgaagcga gccagccggt ggccgctcgc ggccatcgtc cacatatcca
2941 cgggctggca agggagcgca gcgaccgcgc agggcgaagc ccggagagca agcccgtagg
3001 gggggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga
3061 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg
3121 cct
//