LOCUS JQ519689 3546 bp DNA circular SYN 16-JUL-2012
DEFINITION E.coli-K.pastoris shuttle vector pPpT4, complete sequence.
ACCESSION JQ519689
VERSION JQ519689.1
KEYWORDS .
SOURCE Escherichia coli-Pichia pastoris shuttle vector pPpT4
ORGANISM Escherichia coli-Pichia pastoris shuttle vector pPpT4
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3546)
AUTHORS Naatsaari,L., Mistlberger,B., Ruth,C., Hajek,T., Hartner,F.S. and
Glieder,A.
TITLE Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform
Strain Generation for Gene Expression and Synthetic Biology
JOURNAL PLoS ONE 7 (6), E39720 (2012)
PUBMED 22768112
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 3546)
AUTHORS Naeaetsaari,L., Mistlberger,B., Ruth,C., Hajek,T., Hartner,F. and
Glieder,A.
TITLE Direct Submission
JOURNAL Submitted (30-JAN-2012) Institute of Molecular Biotechnology, Graz
University of Technology, Petersgasse 14/2, Graz, Styria 8010,
Austria
COMMENT ##Assembly-Data-START##
Assembly Method :: SeqMan v. 7.0.0
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..3546
/organism="Escherichia coli-Pichia pastoris shuttle vector
pPpT4"
/mol_type="other DNA"
/db_xref="taxon:1182041"
regulatory 139..1069
/regulatory_class="promoter"
/note="AOX1 promoter"
regulatory 1098..1357
/regulatory_class="terminator"
/note="AOX1 terminator"
regulatory 1359..1916
/regulatory_class="promoter"
/note="ILV5 promoter"
regulatory 1917..1980
/regulatory_class="promoter"
/note="EM72 synthetic promoter"
CDS 1982..2356
/codon_start=1
/transl_table=11
/product="zeocin resistance protein"
/protein_id="AFJ20653.1"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQD"
regulatory 2358..2833
/regulatory_class="terminator"
/note="AOD terminator"
rep_origin 2854..3526
/note="pUC origin of replication"
BASE COUNT 900 a 820 c 784 g 1042 t
ORIGIN
1 agatccaatt cccgctttga ctgcctgaaa tctccatcgc ctacaatgat gacatttgga
61 tttggttgac tcatgttggt attgtgaaat agacgcagat cgggaacact gaaaaataca
121 cagttattat tcagatctaa catccaaaga cgaaaggttg aatgaaacct ttttgccatc
181 cgacatccac aggtccattc tcacacataa gtgccaaacg caacaggagg ggatacacta
241 gcagcagacc gttgcaaacg caggacctcc actcctcttc tcctcaacac ccacttttgc
301 catcgaaaaa ccagcccagt tattgggctt gattggagct cgctcattcc aattccttct
361 attaggctac taacaccatg actttattag cctgtctatc ctggcccccc tggcgaggtt
421 catgtttgtt tatttccgaa tgcaacaagc tccgcattac acccgaacat cactccagat
481 gagggctttc tgagtgtggg gtcaaatagt ttcatgttcc ccaaatggcc caaaactgac
541 agtttaaacg ctgtcttgga acctaatatg acaaaagcgt gatctcatcc aagatgaact
601 aagtttggtt cgttgaaatg ctaacggcca gttggtcaaa aagaaacttc caaaagtcgg
661 cataccgttt gtcttgtttg gtattgattg acgaatgctc aaaaataatc tcattaatgc
721 ttagcgcagt ctctctatcg cttctgaacc ccggtgcacc tgtgccgaaa cgcaaatggg
781 gaaacacccg ctttttggat gattatgcat tgtctccaca ttgtatgctt ccaagattct
841 ggtgggaata ctgctgatag cctaacgttc atgatcaaaa tttaactgtt ctaaccccta
901 cttgacagca atatataaac agaaggaagc tgccctgtct taaacctttt tttttatcat
961 cattattagc ttactttcat aattgcgact ggttccaatt gacaagcttt tgattttaac
1021 gacttttaac gacaacttga gaagatcaaa aaacaactaa ttattgaaag aattcactag
1081 tggcgcgccg cggccgctca agaggatgtc agaatgccat ttgcctgaga gatgcaggct
1141 tcatttttga tactttttta tttgtaacct atatagtata ggattttttt tgtcattttg
1201 tttcttctcg tacgagcttg ctcctgatca gcctatctcg cagcagatga atatcttgtg
1261 gtaggggttt gggaaaatca ttcgagtttg atgtttttct tggtatttcc cactcctctt
1321 cagagtacag aagattaagt gagaccttcg tttgtgcgga tccttcagta atgtcttgtt
1381 tcttttgttg cagtggtgag ccattttgac ttcgtgaaag tttctttaga atagttgttt
1441 ccagaggcca aacattccac ccgtagtaaa gtgcaagcgt aggaagacca agactggcat
1501 aaatcaggta taagtgtcga gcactggcag gtgatcttct gaaagtttct actagcagat
1561 aagatccagt agtcatgcat atggcaacaa tgtaccgtgt ggatctaaga acgcgtccta
1621 ctaaccttcg cattcgttgg tccagtttgt tgttatcgat caacgtgaca aggttgtcga
1681 ttccgcgtaa gcatgcatac ccaaggacgc ctgttgcaat tccaagtgag ccagttccaa
1741 caatctttgt aatattagag cacttcattg tgttgcgctt gaaagtaaaa tgcgaacaaa
1801 ttaagagata atctcgaaac cgcgacttca aacgccaata tgatgtgcgg cacacaataa
1861 gcgttcatat ccgctgggtg actttctcgc tttaaaaaat tatccgaaaa aattttctag
1921 agtgttgtta ctttatactt ccggctcgta taatacgaca aggtgtaagg aggactaaac
1981 catggctaaa ctcacctctg ctgttccagt cctgactgct cgtgatgttg ctggtgctgt
2041 tgagttctgg actgatagac tcggtttctc ccgtgacttc gtagaggacg actttgccgg
2101 tgttgtacgt gacgacgtta ccctgttcat ctccgcagtt caggaccagg ttgtgccaga
2161 caacactctg gcatgggtat gggttcgtgg tctggacgaa ctgtacgctg agtggtctga
2221 ggtcgtgtct accaacttcc gtgatgcatc tggtccagct atgaccgaga tcggtgaaca
2281 gccctggggt cgtgagtttg cactgcgtga tccagctggt aactgcgtgc atttcgtcgc
2341 agaagagcag gactaacaat tgacacctta cgattattta gagagtattt attagtttta
2401 ttgtatgtat acggatgttt tattatctat ttatgccctt atattctgta actatccaaa
2461 agtcctatct tatcaagcca gcaatctatg tccgcgaacg tcaactaaaa ataagctttt
2521 tatgctcttc tctctttttt tcccttcggt ataattatac cttgcatcca cagattctcc
2581 tgccaaattt tgcataatcc tttacaacat ggctatatgg gagcacttag cgccctccaa
2641 aacccatatt gcctacgcat gtataggtgt tttttccaca atattttctc tgtgctctct
2701 ttttattaaa gagaagctct atatcggaga agcttctgtg gccgttatat tcggccttat
2761 cgtgggacca cattgcctga attggtttgc cccggaagat tggggaaact tggatctgat
2821 taccttagct gcaggtacca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
2881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
2941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
3001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
3061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
3121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
3181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
3241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
3301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
3361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
3421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
3481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
3541 gtaccc
//