LOCUS JN418198 7438 bp DNA circular SYN 10-JAN-2012
DEFINITION Cloning vector pT2gammaCryK2, complete sequence.
ACCESSION JN418198
VERSION JN418198.1
KEYWORDS .
SOURCE Cloning vector pT2gammaCryK2
ORGANISM Cloning vector pT2gammaCryK2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7438)
AUTHORS Loots,G., Bergmann,A., Hum,N., Hu,N., Oldenburg,C., Khokha,M.,
Ovcharenko,I. and Harland,R.M.
TITLE Interrogating Distal Muscle Enhancers in Tol2-mediated Xenopus
Transgenics
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7438)
AUTHORS Loots,G., Bergmann,A. and Hum,N.
TITLE Direct Submission
JOURNAL Submitted (01-AUG-2011) Biosciences & Biotechnology Division,
Lawrence Livermore National Laboratory, 7000 East Avenue,
Livermore, CA 94550, USA
FEATURES Location/Qualifiers
source 1..7438
/organism="Cloning vector pT2gammaCryK2"
/mol_type="other DNA"
/db_xref="taxon:1127335"
regulatory 13..2434
/regulatory_class="promoter"
/note="Xenopus laevis gamma crystallin lens-specific
promoter (gammaCry)"
gene 2435..3335
/gene="mKate2"
mRNA 2435..3335
/gene="mKate2"
/product="mKate2"
CDS 2435..3133
/gene="mKate2"
/note="far red fluorescent protein; excitation max
588/emission max 633"
/codon_start=1
/transl_table=11
/product="mKate2"
/protein_id="AEX25288.1"
/translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRI
KAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRAD
MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVA
VARYCDLPSKLGHR"
misc_feature 3388..4039
/note="Tol2 transposase recombinant sequence 1"
rep_origin 4430..5068
/note="ColE1 Ori"
gene complement(5220..6080)
/gene="ampR"
CDS complement(5220..6080)
/gene="ampR"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AEX25287.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 6588..7420
/note="Tol2 transposase recombinant sequence 2"
BASE COUNT 2152 a 1642 c 1620 g 2024 t
ORIGIN
1 aattctgcag atatctgcaa agaggggtta actattcttg tgaattattc attctttata
61 tacatcgcag tttatctaag atgggcacct gcatctacaa agcaaaagtg ttgtacatca
121 acaaatagca ctctgcaggc aagcttgggg cgcgcaatta accctcacta aagggaacaa
181 aagctggtac cgggcccccc ctcgagtcca taagaaagat ataaataagc tggagagaat
241 gcagaattgt caactagcag tgctggaatt ggggagggat gccatcccct cactttttta
301 tttctgtgaa atagcatccc ctcagtgtgg atgcaagttt tttgaatccc ggaactgtct
361 tttgctcctc ttcacttctg catgcaaata caatgcagag gtaaaatata atatagttaa
421 atatataggg gattaaagga gagaaaaaat tgctgggaga gaagaagaaa agatagagaa
481 gtgttaaaca gataaagaag gtggaggcat cagagagggt tatagagaaa aggagagaag
541 aaaaaatgaa ggtattaaac agcagcagat taaggaatag tgagaaaatg ctttatatga
601 aataatcttg ggtaatatag aatatgatgg ggatgtgcta tgttatgaca caatacacag
661 aaaaggtgct ttatttgcag tagctgaatt ggtagccaag aaaagtcata agcacaattt
721 ttattttggg gtccctatag gtcacatatg taaatatggt tatgggaggg atgcaccaca
781 cagagcatcc ctccactttt tttactccaa ttcaagcact gggaatgaga tatgcttcat
841 tagcacctaa acatttgaca atgaccaatg tgaatgttag cgaccatatt tgcgtccttt
901 tgtgaccaaa tggcatatgt gaacattcct agtgtatgta ataaaatttt gcaacaaaat
961 atttaaaaac tccagagcag atgcttaact tagtcatgta atattcgcca gcaaatgagt
1021 atacctctat ttttaacatc acttgtgatt tttttagtaa cattcccacc tttagtattt
1081 ccagattgaa attatcatac ccccccattc tttaatatag ggaacactgt accagtgggt
1141 ctgggattat ttgtccatat attgcaatat atccaagctg accaactgtc tcaaagtcac
1201 ccctggtctg gccaataaaa cacaaacatt ctagcagaaa gtaaagctgg ccatagatgt
1261 tgagattttt aaaagatcag atcctgatcg tgagaccacg atcttctcag aacgatcgta
1321 cgatcgtacg aatctaccat caactaaaaa gaccaattta ccaggaaaac aaaggggagc
1381 tgcctgcttg gccctgcaaa catagagaga ttgcactggg gccgacaaag attttttgac
1441 ctggccgatc aatttcccga cagatgtcgg ccgaaaaatc gtaagatgta cgatcgttcg
1501 aataccacta accgcacgat aatttcgaag gattggtcgg gcttccctaa aatcggtcgt
1561 tcggcaagaa gaatcgtcgc gtctatgggg accttaagtg tacttgcttt ccaaggttaa
1621 aaattgttac ctttttatac gacactgcat ggatcacctg aaagcaggtt gggagctaca
1681 acatggaact agccactgtt cctttatatg gttatcacta cccaatttta aataattaaa
1741 ctggagttta ttgatggtgt gaccacaaaa ttgatacaga taaagacata ctgtatatat
1801 tgcgttatat gacaaataca gtaacaccac attactattt taaattatat ataaaataat
1861 attaatatat ctggggacat aaaatgtggc ttcttaaaca ttggggtgac actgatgaga
1921 ttatttggta aatgtttgat gctagctaac cattgtttgg ttcatttgtg tgattcaaaa
1981 gtggcaaaga atgacaccta gatgatgcca aattaaggat ttactagtaa ccacaattct
2041 ttaaactgtt acaaagtgtt tggaccagca actgaattgc actgtaaatt accacatcag
2101 ggtctccggg gtcaacgctg ctaagcaatg gactggaaaa tttgtgacat tgagtttttg
2161 gaataactgg gaatgctgga taactctttg ggtaaagcta tggaacaaaa gtaccagcat
2221 gaactaagaa atgactgtca cagcacaaca tcaaacacaa tgcaagacaa aggttctctg
2281 cttttgcctt acttgacaga aaaagtaagc tctaggttca tcgcatgcag acagcagcaa
2341 tacatgacat atataaaggc tggctctttt ctcccttcca ttgaactgaa acttccactc
2401 agtcagactt gcgggggatc caccggtcgc caccatggtg agcgagctga ttaaggagaa
2461 catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
2521 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg cggtcgaggg
2581 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac
2641 cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc ccgagggctt
2701 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
2761 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc
2821 caacggccct gtgatgcaga agaaaacact cggctgggag gcctccaccg agaccctgta
2881 ccccgctgac ggcggcctgg aaggcagagc cgacatggcc ctgaagctcg tgggcggggg
2941 ccacctgatc tgcaacttga agaccacata cagatccaag aaacccgcta agaacctcaa
3001 gatgcccggc gtctactatg tggacagaag actggaaaga atcaaggagg ccgacaaaga
3061 gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact
3121 ggggcacaga tgagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
3181 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
3241 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
3301 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
3361 tcatcaatgt atcttaatcg agggcccatc tggcctgtgt ttcagacacc agggagtctc
3421 tgctcacgtt tcctgctatt tgcagcctct ctatcaagac taatacacct cttcccgcat
3481 cggctgcctg tgagaggctt ttcagcactg caggattgct tttcagcccc aaaagagcta
3541 ggcttgacac taacaatttt gagaatcagc ttctactgaa gttaaatctg aggttttaca
3601 actttgagta gcgtgtactg gcattagatt gtctgtctta tagtttgata attaaataca
3661 aacagttcta aagcaggata aaaccttgta tgcatttcat ttaatgtttt ttgagattaa
3721 aagcttaaac aagaatctct agttttcttt cttgctttta cttttacttc cttaatactc
3781 aagtacaatt ttaatggagt acttttttac ttttactcaa gtaagattct agccagatac
3841 ttttactttt aattgagtaa aattttccct aagtacttgt actttcactt gagtaaaatt
3901 tttgagtact ttttacacct ctgtcaagaa ctcctggaca aacctctgac ctgtgtggaa
3961 cagagtggat atgggtgtct gaacagatat tcacgtcttt tgcagatcag agggcatttc
4021 tggtgttgtg caatgacctg ggtccaacag gtaggcgtac tcgtatccaa gagcattgtt
4081 ggacaggagg tagtctccat tcctataaag aggaaggaat ggcaccatgt agtagccgtc
4141 attgtggcca attggtgcat tggtacatca gatctgccgg tctccctata gtgagtcgta
4201 ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc
4261 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
4321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
4381 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
4441 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
4501 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
4561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
4621 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
4681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
4741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
4801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
4861 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
4921 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
4981 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
5041 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
5101 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
5161 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
5221 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
5281 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
5341 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
5401 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
5461 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
5521 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
5581 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
5641 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
5701 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
5761 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
5821 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
5881 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
5941 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
6001 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
6061 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
6121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
6181 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
6241 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
6301 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
6361 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
6421 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg
6481 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
6541 cactatagaa ctcgagcagc tgaagcttgc atgcctgcag gtcgactcta gctctagaac
6601 tagtggatct gctgggcttg ctgaaggtag ggggtcaaga accagaggtg taaagtactt
6661 gagtaatttt acttgattac tgtacttaag tattattttt ggggattttt actttacttg
6721 agtacaatta aaaatcaata cttttacttt tacttaatta cattttttta gaaaaaaaag
6781 tactttttac tccttacaat tttatttaca gtcaaaaagt acttattttt tggagatcac
6841 ttcattctat tttcccttgc tattaccaaa ccaattgaat tgcgctgatg cccagtttaa
6901 tttaaatgtt atttattctg cctatgaaaa tcgttttcac attatatgaa attggtcaga
6961 catgttcatt ggtcctttgg aagtgacgtc atgtcacatc tattaccaca atgcacagca
7021 ccttgacctg gaaattaggg aaattataac agtcaatcag tggaagaaaa tggaggaagt
7081 atgtgattca tcagcagctg cgagcagcac agtccaaaat cagccacagg atcaagagca
7141 cccgtggccg tatcttcgca gatctgatct agaggatcat aatcagccat accacatttg
7201 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
7261 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
7321 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
7381 ccaaactcat caatgtatct tatcatgtct ggatcatcat cgtcgagctc aagcttcg
//