LOCUS JF420887 8646 bp DNA circular SYN 17-MAY-2011
DEFINITION Lux single copy cloning vector pMH32 luxCDABE operon, complete
sequence; and chloramphenicol transacetylase (camR) gene, complete
cds.
ACCESSION JF420887
VERSION JF420887.1
KEYWORDS .
SOURCE Lux single copy cloning vector pMH32
ORGANISM Lux single copy cloning vector pMH32
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8646)
AUTHORS Sitaras,C., Beyde,A., Malekazari,P. and Herrington,M.B.
TITLE Light producing reporter plasmids for Escherichia coli K-12 that
can be integrated into the chromosome
JOURNAL Plasmid 65 (3), 232-238 (2011)
PUBMED 21376749
REFERENCE 2 (bases 1 to 8646)
AUTHORS Herrington,M.B.
TITLE Direct Submission
JOURNAL Submitted (23-FEB-2011) Concordia University, 7141 Sherbrooke St.
W., Montreal, QC H4B 1G2, Canada
FEATURES Location/Qualifiers
source 1..8646
/organism="Lux single copy cloning vector pMH32"
/mol_type="other DNA"
/db_xref="taxon:989307"
operon 138..5935
/operon="luxCDABE"
gene 138..1580
/gene="luxC"
/operon="luxCDABE"
CDS 138..1580
/gene="luxC"
/operon="luxCDABE"
/note="derived from Photorhabdus luminescens sequence"
/codon_start=1
/transl_table=11
/product="fatty acid reductase"
/protein_id="ADZ46400.1"
/translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVK
NIIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEAN
WISMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGI
MSILRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSL
AKEIMRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGA
AHDVCFYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSL
VQKESLFAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNK
TQTISIFPWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHE
RPSNYTAKDVAVEIEQTRFLEEDKFLVFVP"
gene 1592..2515
/gene="luxD"
/operon="luxCDABE"
CDS 1592..2515
/gene="luxD"
/operon="luxCDABE"
/note="derived from Photorhabdus luminescens sequence"
/codon_start=1
/transl_table=11
/product="acyl transferase"
/protein_id="ADZ46402.1"
/translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIAS
GFARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWL
TTRKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPI
NELPDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQ
DEVITLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVD
ITEPSFEHLTIATVNERRMRIEIENQAISLS"
gene 2564..3646
/gene="luxA"
/operon="luxCDABE"
CDS 2564..3646
/gene="luxA"
/operon="luxCDABE"
/note="derived from Photorhabdus luminescens sequence"
/codon_start=1
/transl_table=11
/product="luciferase alpha subunit"
/protein_id="ADZ46401.1"
/translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEH
HFTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFR
FGICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNP
AAYSRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHD
IHNIDHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQ
WRDFVLKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVD
EIIASMKLFQSDVMPFLKEKQRSLLY"
gene 3661..4644
/gene="luxB"
/operon="luxCDABE"
CDS 3661..4644
/gene="luxB"
/operon="luxCDABE"
/note="derived from Photorhabdus luminescens sequence"
/codon_start=1
/transl_table=11
/product="luciferase beta subunit"
/protein_id="ADZ46403.1"
/translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYEN
HFSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFI
LGFSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNP
HAYTPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVD
LSEIDHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNY
TECITAAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
gene 4823..5935
/gene="luxE"
/operon="luxCDABE"
CDS 4823..5935
/gene="luxE"
/operon="luxCDABE"
/note="derived from Photorhabdus luminescens sequence"
/codon_start=1
/transl_table=11
/product="acyl-protein synthetase"
/protein_id="ADZ46398.1"
/translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDA
FRNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSS
GTNGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYV
MSLVELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKK
ISFSGDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTC
FFEDEMQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGII
SREYGKYPGVLVEILRRVNTRTQKGCALSLTEAFDS"
misc_recomb 6134..6381
/note="site specific recombination between attP and attB
in Escherichia coli"
rep_origin 6664..7048
gene complement(7619..8278)
/gene="camR"
CDS complement(7619..8278)
/gene="camR"
/codon_start=1
/transl_table=11
/product="chloramphenicol transacetylase"
/protein_id="ADZ46399.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
BASE COUNT 2651 a 1542 c 1844 g 2609 t
ORIGIN
1 gccccagctg gcaattccga cgtctaagaa accattatta tcatgacatt aacctataaa
61 aataggcgta tcacgaggcc ctttcgtctt cacctcgagg ggatcctcta gttgcggccg
121 caaaatggat ggcaaatatg actaaaaaaa tttcattcat tattaacggc caggttgaaa
181 tctttcccga aagtgatgat ttagtgcaat ccattaattt tggtgataat agtgtttacc
241 tgccaatatt gaatgactct catgtaaaaa acattattga ttgtaatgga aataacgaat
301 tacggttgca taacattgtc aattttctct atacggtagg gcaaagatgg aaaaatgaag
361 aatactcaag acgcaggaca tacattcgtg acttaaaaaa atatatggga tattcagaag
421 aaatggctaa gctagaggcc aattggatat ctatgatttt atgttctaaa ggcggccttt
481 atgatgttgt agaaaatgaa cttggttctc gccatatcat ggatgaatgg ctacctcagg
541 atgaaagtta tgttcgggct tttccgaaag gtaaatctgt acatctgttg gcaggtaatg
601 ttccattatc tgggatcatg tctatattac gcgcaatttt aactaagaat cagtgtatta
661 taaaaacatc gtcaaccgat ccttttaccg ctaatgcatt agcgttaagt tttattgatg
721 tagaccctaa tcatccgata acgcgctctt tatctgttat atattggccc caccaaggtg
781 atacatcact cgcaaaagaa attatgcgac atgcggatgt tattgtcgct tggggagggc
841 cagatgcgat taattgggcg gtagagcatg cgccatctta tgctgatgtg attaaatttg
901 gttctaaaaa gagtctttgc attatcgata atcctgttga tttgacgtcc gcagcgacag
961 gtgcggctca tgatgtttgt ttttacgatc agcgagcttg tttttctgcc caaaacatat
1021 attacatggg aaatcattat gaggaattta agttagcgtt gatagaaaaa cttaatctat
1081 atgcgcatat attaccgaat gccaaaaaag attttgatga aaaggcggcc tattctttag
1141 ttcaaaaaga aagcttgttt gctggattaa aagtagaggt ggatattcat caacgttgga
1201 tgattattga gtcaaatgca ggtgtggaat ttaatcaacc acttggcaga tgtgtgtacc
1261 ttcatcacgt cgataatatt gagcaaatat tgccttatgt tcaaaaaaat aagacgcaaa
1321 ccatatctat ttttccttgg gagtcatcat ttaaatatcg agatgcgtta gcattaaaag
1381 gtgcggaaag gattgtagaa gcaggaatga ataacatatt tcgagttggt ggatctcatg
1441 acggaatgag accgttgcaa cgattagtga catatatttc tcatgaaagg ccatctaact
1501 atacggctaa ggatgttgcg gttgaaatag aacagactcg attcctggaa gaagataagt
1561 tccttgtatt tgtcccataa taggtaaaag tatggaaaat gaatcaaaat ataaaaccat
1621 cgaccacgtt atttgtgttg aaggaaataa aaaaattcat gtttgggaaa cgctgccaga
1681 agaaaacagc ccaaagagaa agaatgccat tattattgcg tctggttttg cccgcaggat
1741 ggatcatttt gctggtctgg cggaatattt atcgcggaat ggatttcatg tgatccgcta
1801 tgattcgctt caccacgttg gattgagttc agggacaatt gatgaattta caatgtctat
1861 aggaaagcag agcttgttag cagtggttga ttggttaact acacgaaaaa taaataactt
1921 cggtatgttg gcttcaagct tatctgcgcg gatagcttat gcaagcctat ctgaaatcaa
1981 tgcttcgttt ttaatcaccg cagtcggtgt tgttaactta agatattctc ttgaaagagc
2041 tttagggttt gattatctca gtctacccat taatgaattg ccggataatc tagattttga
2101 aggccataaa ttgggtgctg aagtctttgc gagagattgt cttgattttg gttgggaaga
2161 tttagcttct acaattaata acatgatgta tcttgatata ccgtttattg cttttactgc
2221 aaataacgat aattgggtca agcaagatga agttatcaca ttgttatcaa atattcgtag
2281 taatcgatgc aagatatatt ctttgttagg aagttcgcat gacttgagtg aaaatttagt
2341 ggtcctgcgc aatttttatc aatcggttac gaaagccgct atcgcgatgg ataatgatca
2401 tctggatatt gatgttgata ttactgaacc gtcatttgaa catttaacta ttgcgacagt
2461 caatgaacgc cgaatgagaa ttgagattga aaatcaagca atttctctgt cttaaaatct
2521 attgagatat tctatcactc aaatagcaat ataaggactc tctatgaaat ttggaaactt
2581 tttgcttaca taccaacctc cccaattttc tcaaacagag gtaatgaaac gtttggttaa
2641 attaggtcgc atctctgagg agtgtggttt tgataccgta tggttactgg agcatcattt
2701 cacggagttt ggtttgcttg gtaaccctta tgtcgctgct gcatatttac ttggcgcgac
2761 taaaaaattg aatgtaggaa ctgccgctat tgttcttccc acagcccatc cagtacgcca
2821 acttgaagat gtgaatttat tggatcaaat gtcaaaagga cgatttcggt ttggtatttg
2881 ccgagggctt tacaacaagg actttcgcgt attcggcaca gatatgaata acagtcgcgc
2941 cttagcggaa tgctggtacg ggctgataaa gaatggcatg acagagggat atatggaagc
3001 tgataatgaa catatcaagt tccataaggt aaaagtaaac cccgcggcgt atagcagagg
3061 tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg actgagtggg ctgctcaatt
3121 tggcctaccg atgatattaa gttggattat aaatactaac gaaaagaaag cacaacttga
3181 gctttataat gaagtggctc aagaatatgg gcacgatatt cataatatcg accattgctt
3241 atcatatata acatctgtag atcatgactc aattaaagcg aaagagattt gccggaaatt
3301 tctggggcat tggtatgatt cttatgtgaa tgctacgact atttttgatg attcagacca
3361 aacaagaggt tatgatttca ataaagggca gtggcgtgac tttgtattaa aaggacataa
3421 agatactaat cgccgtattg attacagtta cgaaatcaat cccgtgggaa cgccgcagga
3481 atgtattgac ataattcaaa aagacattga tgctacagga atatcaaata tttgttgtgg
3541 atttgaagct aatggaacag tagacgaaat tattgcttcc atgaagctct tccagtctga
3601 tgtcatgcca tttcttaaag aaaaacaacg ttcgctatta tattagctaa ggagaaagaa
3661 atgaaatttg gattgttctt ccttaacttc atcaattcaa caactgttca agaacaaagt
3721 atagttcgca tgcaggaaat aacggagtat gttgataagt tgaattttga acagatttta
3781 gtgtatgaaa atcatttttc agataatggt gttgtcggcg ctcctctgac tgtttctggt
3841 tttctgctcg gtttaacaga gaaaattaaa attggttcat taaatcacat cattacaact
3901 catcatcctg tcgccatagc ggaggaagct tgcttattgg atcagttaag tgaagggaga
3961 tttattttag ggtttagtga ttgcgaaaaa aaagatgaaa tgcatttttt taatcgcccg
4021 gttgaatatc aacagcaact atttgaagag tgttatgaaa tcattaacga tgctttaaca
4081 acaggctatt gtaatccaga taacgatttt tatagcttcc ctaaaatatc tgtaaatccc
4141 catgcttata cgccaggcgg acctcggaaa tatgtaacag caaccagtca tcatattgtt
4201 gagtgggcgg ccaaaaaagg tattcctctc atctttaagt gggatgattc taatgatgtt
4261 agatatgaat atgctgaaag atataaagcc gttgcggata aatatgacgt tgacctatca
4321 gagatagacc atcagttaat gatattagtt aactataacg aagatagtaa taaagctaaa
4381 caagagacgc gtgcatttat tagtgattat gttcttgaaa tgcaccctaa tgaaaatttc
4441 gaaaataaac ttgaagaaat aattgcagaa aacgctgtcg gaaattatac ggagtgtata
4501 actgcggcta agttggcaat tgaaaagtgt ggtgcgaaaa gtgtattgct gtcctttgaa
4561 ccaatgaatg atttgatgag ccaaaaaaat gtaatcaata ttgttgatga taatattaag
4621 aagtaccaca tggaatatac ctaatagatt tcgagttgca gcgaggcggc aagtgaacga
4681 atccccagga gcatagataa ctatgtgact ggggtgagtg aaagcagcca acaaagcagc
4741 agcttgaaag atgaagggta taaaagagta tgacagcagt gctgccatac tttctaatat
4801 tatcttgagg agtaaaacag gtatgacttc atatgttgat aaacaagaaa ttacagcaag
4861 ctcagaaatt gatgatttga ttttttcgag cgatccatta gtgtggtctt acgacgagca
4921 ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt aatcattata aacattgtcg
4981 agaatatcgt cactactgtc aggcacacaa agtagatgac aatattacgg aaattgatga
5041 catacctgta ttcccaacat cggtttttaa gtttactcgc ttattaactt ctcaggaaaa
5101 cgagattgaa agttggttta ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg
5161 tgacagatta agtattgaga gactcttagg ctctgtgagt tatggcatga aatatgttgg
5221 tagttggttt gatcatcaaa tagaattagt caatttggga ccagatagat ttaatgctca
5281 taatatttgg tttaaatatg ttatgagttt ggtggaattg ttatatccta cgacatttac
5341 cgtaacagaa gaacgaatag attttgttaa aacattgaat agtcttgaac gaataaaaaa
5401 tcaagggaaa gatctttgtc ttattggttc gccatacttt atttatttac tctgccatta
5461 tatgaaagat aaaaaaatct cattttctgg agataaaagc ctttatatca taaccggagg
5521 cggctggaaa agttacgaaa aagaatctct gaaacgtgat gatttcaatc atcttttatt
5581 tgatactttc aatctcagtg atattagtca gatccgagat atatttaatc aagttgaact
5641 caacacttgt ttctttgagg atgaaatgca gcgtaaacat gttccgccgt gggtatatgc
5701 gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag
5761 ttatatggat gcgtcagcaa ccagttatcc agcatttatt gttaccgatg atgtcgggat
5821 aattagcaga gaatatggta agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa
5881 tacgaggacg cagaaagggt gtgctttaag cttaaccgaa gcgtttgata gttgatatcc
5941 tttgcctgcg gccgcaacta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
6001 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
6061 ccctagacct agccttaatt aaccatggca tcacagtatc gtgatgacag aggcagggag
6121 tgggacaaaa ttgaaatcaa ataatgattt tattttgact gatagtgacc tgttcgttgc
6181 aacaaattga taagcaatgc ttttttataa tgccaactta gtataaaaaa gctgaacgag
6241 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
6301 cataatactg taaaacacaa catatgcagt cactatgaat caactactta gatggtatta
6361 gtgacctgta acagagcatt agcgcaaggt gtttttgtct tcttgcgcta attttttgtc
6421 atcaaacctg tcgcactcca gagaagcaca aagcctcgca atccagtgca aagctgaatt
6481 ggggatcatt tattctgcct cccagagcct gataaaaacg gtgaatccgt tagcgaggtg
6541 ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgattat tgaagcattt
6601 atcagggtta ttgtctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
6661 catgtcagcc gttaagtgtt cctgtgtcac tgaaaattgc tttgagaggc tctaagggct
6721 tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa aagctagctt
6781 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag
6841 ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga
6901 gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac
6961 atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
7021 tactatcaac aggttgaact gctgatcgat ctcacatgtt ctttcctgcg ttatcccctg
7081 attctgttaa ttaaggtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc
7141 tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt
7201 gttttttttg tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag
7261 ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat
7321 tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa
7381 ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt tgtctccgac
7441 catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc tcacggctct
7501 ggcagtgaat gggggtaaat ggcactacag gccgaatttc tgccattcat ccgcttatta
7561 tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt
7621 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
7681 ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
7741 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
7801 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
7861 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
7921 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
7981 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
8041 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa
8101 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
8161 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
8221 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
8281 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
8341 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa
8401 agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt
8461 gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac
8521 tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt
8581 ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag
8641 cgctag
//