LOCUS HM623915 4833 bp DNA circular SYN 10-JUN-2011
DEFINITION Marker vector pA-hyg-OSCAR, complete sequence.
ACCESSION HM623915
VERSION HM623915.1
KEYWORDS .
SOURCE Marker vector pA-hyg-OSCAR
ORGANISM Marker vector pA-hyg-OSCAR
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4833)
AUTHORS Paz,Z., Garcia-Pedrajas,M.D., Andrews,D.L., Klosterman,S.J.,
Baeza-Montanez,L. and Gold,S.E.
TITLE One Step Construction of Agrobacterium-Recombination-ready-plasmids
(OSCAR), an efficient and robust tool for ATMT based gene deletion
construction in fungi
JOURNAL Fungal Genet. Biol. 48 (7), 677-684 (2011)
PUBMED 21362493
REFERENCE 2 (bases 1 to 4833)
AUTHORS Paz,Z., Garcia-Pedrajas,M.D., Andrews,D.L., Klosterman,S.J.,
Baeza-Montanez,L. and Gold,S.E.
TITLE Direct Submission
JOURNAL Submitted (01-JUL-2010) Plant Pathology, The University of Georgia,
2105 Miller Plant Sci Bldg, Athens, GA 30602-7274, USA
FEATURES Location/Qualifiers
source 1..4833
/organism="Marker vector pA-hyg-OSCAR"
/mol_type="other DNA"
/db_xref="taxon:932666"
misc_recomb 678..909
/note="attp4"
CDS complement(959..1984)
/codon_start=1
/transl_table=11
/product="hph"
/protein_id="ADR73033.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory complement(1991..2338)
/regulatory_class="promoter"
/note="trpC"
misc_recomb 2361..2592
/note="attP1r"
BASE COUNT 1224 a 1209 c 1188 g 1212 t
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggtgcc ggaattctac aggtcactaa taccatctaa gtagttgatt catagtgact
721 gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
781 tattgatatt tatatcattt tacgtttctc gttcaacttt tctatacaaa gttggcatta
841 taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
901 tcattatttg gccgctctag aactagtgga tccaactggt tcccggtcgg catctactct
961 attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
1021 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
1081 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
1141 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
1201 ggcgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
1261 tacaagccaa ccacggcctc cagaagagga tgttggcgac ctcgtattgg gaatccccga
1321 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
1381 tggagccgaa atccgcatgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
1441 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
1501 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
1561 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
1621 tcgcatccat ggcctccgcg accggctgga gaacagcggg cagttcggtt tcaggcaggt
1681 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaact
1741 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
1801 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
1861 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
1921 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
1981 tcatttggat gcttgggtag aataggtaag tcagattgaa tctgaaataa agggaggaag
2041 ggcgaactta agaaggtatg accgggtcgt ccacttacct tgcttgacaa acgcaccaag
2101 ttatcgtgca ccaagcagca gatgataata atgtcctcgt tcctgtctgc taataagagt
2161 cacacttcga gcgccgccgc tactgctaca agtggggctg atctgaccag ttgcctaaat
2221 gaaccatctt gtcaaacgac acaaattttg tgctcaccgc ctggacgact aaaccaaaat
2281 aggcattcat tgttgacctc cactagctcc agccaagccc aaaaaatgct ccttcaatat
2341 cagttgggct gcaggaattc tacaggtcac taataccatc taagtagttg attcatagtg
2401 actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
2461 atatattgat atttatatca ttttacgttt ctcgttcaac ttttttgtac aaagttggca
2521 ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
2581 aaatcattat ttggagctct agagcgtcga cctcgagggg gggcccggta cccagctttt
2641 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
2701 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
2761 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
2821 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
2881 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
2941 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
3001 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
3061 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
3121 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
3181 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
3241 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
3301 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
3361 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
3421 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
3481 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
3541 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
3601 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
3661 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
3721 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
3781 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
3841 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
3901 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
3961 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
4021 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
4081 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
4141 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
4201 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
4261 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
4321 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
4381 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
4441 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
4501 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
4561 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
4621 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
4681 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
4741 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
4801 gggttccgcg cacatttccc cgaaaagtgc cac
//