LOCUS       HM588138                9651 bp    DNA     linear   SYN 08-NOV-2010
DEFINITION  Targeting vector R26-Stop-HA3-ICA512-CCF, partial sequence.
ACCESSION   HM588138
VERSION     HM588138.1
KEYWORDS    .
SOURCE      Targeting vector R26-Stop-HA3-ICA512-CCF
  ORGANISM  Targeting vector R26-Stop-HA3-ICA512-CCF
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Liu,Y., Suckale,J., Masjkur,J., Magro,M.G., Steffen,A.,
            Anastassiadis,K. and Solimena,M.
  TITLE     Tamoxifen-Independent Recombination in the RIP-CreER Mouse
  JOURNAL   PLoS ONE 5 (10), E13533 (2010)
   PUBMED   21063464
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  AUTHORS   Suckale,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2010) Paul Langerhans Institute, Dresden
            University of Technology, Fiedlerstr. 42, Dresden, Saxony 01307,
            Germany
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Targeting vector R26-Stop-HA3-ICA512-CCF"
                     /mol_type="other DNA"
                     /db_xref="taxon:885264"
                     /note="targeting vector for homologous recombination into
                     endogenous locus (mouse and foreign sequences);
                     Cre-activated expression of Ptprn (ICA512) cleaved
                     cytosolic fragment from the ROSA26 locus"
     gene            <1..>1090
                     /gene="Rosa26"
     misc_feature    <1..>1090
                     /gene="Rosa26"
                     /note="5' homology arm"
     regulatory      1091..1208
                     /regulatory_class="other"
                     /note="splice acceptor"
     misc_recomb     1215..1248
                     /note="loxP"
     CDS             1791..2594
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin resistance"
                     /protein_id="ADN52072.1"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      3152..3343
                     /regulatory_class="polyA_signal_sequence"
     regulatory      3406..3597
                     /regulatory_class="polyA_signal_sequence"
     regulatory      3660..3851
                     /regulatory_class="polyA_signal_sequence"
     misc_recomb     3884..3917
                     /note="loxP"
     CDS             3943..5019
                     /note="3xHA-ICA512 cleaved cytosolic fragment"
                     /codon_start=1
                     /transl_table=11
                     /product="PTPRN-CCF"
                     /protein_id="ADN52073.1"
                     /translation="MARYPYDVPDYAGYPYDVPDYAGSYPYDVPDYASRDLSQFSDAA
                     QASPSSHSSTPSWCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEP
                     NTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYI
                     ATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNL
                     VSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKC
                     YRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRS
                     KDQFEFALTAVAEEVNAILKALPQ"
     gene            <5308..>9651
                     /gene="Rosa26"
     misc_feature    5308..9651
                     /gene="Rosa26"
                     /note="3' homology arm"
BASE COUNT         2305 a         2288 c         2365 g         2693 t
ORIGIN      
        1 gccagcggca ggccctccga gcgtggtgga gccgttctgt gagacagccg ggtacgagtc
       61 gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc ggtccgccct gcagcaaccg
      121 gagggggagg gagaagggag cggaaaagtc tccaccggac gcggccatgg ctcggggggg
      181 ggggggcagc ggaggagcgc ttccggccga cgtctcgtcg ctgattggct tcttttcctc
      241 ccgccgtgtg tgaaaacaca aatggcgtgt tttggttggc gtaaggcgcc tgtcagttaa
      301 cggcagccgg agtgcgcagc cgccggcagc ctcgctctgc ccactgggtg gggcgggagg
      361 taggtggggt gaggcgagct ggacgtgcgg gcgcggtcgg cctctggcgg ggcgggggag
      421 gggagggagg gtcagcgaaa gtagctcgcg cgcgagcggc cgcccaccct ccccttcctc
      481 tgggggagtc gttttacccg ccgccggccg ggcctcgtcg tctgattggc tctcggggcc
      541 cagaaaactg gcccttgcca ttggctcgtg ttcgtgcaag ttgagtccat ccgccggcca
      601 gcgggggcgg cgaggaggcg ctcccaggtt ccggccctcc cctcggcccc gcgccgcaga
      661 gtctggccgc gcgcccctgc gcaacgtggc aggaagcgcg cgctgggggc ggggacgggc
      721 agtagggctg agcggctgcg gggcgggtgc aagcacgttt ccgacttgag ttgcctcaag
      781 aggggcgtgc tgagccagac ctccatcgcg cactccgggg agtggaggga aggagcgagg
      841 gctcagttgg gctgttttgg aggcaggaag cacttgctct cccaaagtcg ctctgagttg
      901 ttatcagtaa gggagctgca gtggagtagg cggggagaag gccgcaccct tctccggagg
      961 ggggagggga gtgttgcaat acctttctgg gagttctctg ctgcctcctg gcttctgagg
     1021 accgccctgg gcctgggaga atcccttgcc ccctcttccc ctcgtgatct gcaactccag
     1081 tctttctagc gtgacctgca cgtctagggc gcagtagtcc agggtttcct tgatgatgtc
     1141 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc
     1201 tttccagtcc gcggataact tcgtatagca tacattatac gaagttatta ggtccctcga
     1261 cctgcaggaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     1321 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     1381 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     1441 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     1501 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     1561 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     1621 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     1681 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     1741 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagccaat atgggatcgg
     1801 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     1861 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     1921 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     1981 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     2041 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     2101 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     2161 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     2221 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     2281 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     2341 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     2401 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     2461 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     2521 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     2581 acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct attaaacaat
     2641 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca
     2701 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta
     2761 gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag
     2821 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc
     2881 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt
     2941 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca
     3001 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat
     3061 cagaagcttg cagatctgcg actctagagg atctgcgact ctagaggatc ataatcagcc
     3121 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     3181 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     3241 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3301 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc gactctagag
     3361 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3421 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     3481 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3541 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     3601 ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     3661 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     3721 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3781 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3841 tatcatgtct ggatccccat caagctgatc cggaaccctt aatataactt cgtatagcat
     3901 acattatacg aagttatagc tagattcgaa cccggtgcca ccatggcgag atacccatac
     3961 gatgtccctg actatgcggg ctatccctat gacgtcccgg actatgcagg atcctaccca
     4021 tatgacgttc cagattacgc ttcaagagat ctctcccagt tcagcgacgc agcccaggcc
     4081 agccccagct cccacagcag caccccgtcc tggtgcgagg agccggccca agccaacatg
     4141 gacatctcca cgggacacat gattctggca tacatggagg atcacctgcg gaaccgggac
     4201 cgccttgcca aggagtggca ggccctctgt gcctaccaag cagagccaaa cacctgtgcc
     4261 accgcgcagg gggagggcaa catcaaaaag aaccggcatc ctgacttcct gccctatgac
     4321 catgcccgca taaaactgaa ggtggagagc agcccttctc ggagcgatta catcaacgcc
     4381 agccccatta ttgagcatga ccctcggatg ccagcctaca tagccacgca gggcccgctg
     4441 tcccatacca tcgcagactt ctggcagatg gtgtgggaga gcggctgcac cgtcatcgtc
     4501 atgctgaccc cgctggtgga ggatggtgtc aagcagtgtg accgctactg gccagatgag
     4561 ggtgcctccc tctaccacgt atatgaggtg aacctggtgt cggagcacat ctggtgcgag
     4621 gactttctgg tgcggagctt ctacctgaag aacgtgcaga cccaggagac gcgcacgctc
     4681 acgcagttcc acttcctcag ctggccggca gagggcacac cggcctccac gcggcccctg
     4741 ctggacttcc gcaggaaggt gaacaagtgc taccggggcc gctcctgccc catcatcgtg
     4801 cactgcagtg atggtgcggg gaggaccggc acctacatcc tcatcgacat ggtcctgaac
     4861 cgcatggcaa aaggagtgaa ggagattgac atcgctgcca ccctggagca tgtccgtgac
     4921 cagcggcctg gccttgtccg ctctaaggac cagtttgaat ttgccctgac agccgtggcg
     4981 gaggaagtga atgccatcct caaggccctg ccccagtgac gaattggggg gaggctaact
     5041 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     5101 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     5161 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     5221 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     5281 gcaggccctg ccatagacgc gtgctagaag atgggcggga gtcttctggg caggcttaaa
     5341 ggctaacctg gtgtgtgggc gttgtcctgc aggggaattg aacaggtgta aaattggagg
     5401 gacaagactt cccacagatt ttcggttttg tcgggaagtt ttttaatagg ggcaaataag
     5461 gaaaatggga ggataggtag tcatctgggg ttttatgcag caaaactaca ggttattatt
     5521 gcttgtgatc cgcctcggag tattttccat cgaggtagat taaagacatg ctcacccgag
     5581 ttttatactc tcctgcttga gatccttact acagtatgaa attacagtgt cgcgagttag
     5641 actatgtaag cagaatttta atcattttta aagagcccag tacttcatat ccatttctcc
     5701 cgctccttct gcagccttat caaaaggtat tttagaacac tcattttagc cccattttca
     5761 tttattatac tggcttatcc aacccctaga cagagcattg gcattttccc tttcctgatc
     5821 ttagaagtct gatgactcat gaaaccagac agattagtta catacaccac aaatcgaggc
     5881 tgtagctggg gcctcaacac tgcagttctt ttataactcc ttagtacact ttttgttgat
     5941 cctttgcctt gatccttaat tttcagtgtc tatcacctct cccgtcaggt ggtgttccac
     6001 atttgggcct attctcagtc cagggagttt tacaacaata gatgtattga gaatccaacc
     6061 taaagcttaa ctttccactc ccatgaatgc ctctctcctt tttctccatt tataaactga
     6121 gctattaacc attaatggtt tccaggtgga tgtctcctcc cccaatatta cctgatgtat
     6181 cttacatatt gccaggctga tattttaaga cattaaaagg tatatttcat tattgagcca
     6241 catggtattg attactgctt actaaaattt tgtcattgta cacatctgta aaaggtggtt
     6301 ccttttggaa tgcaaagttc aggtgtttgt tgtctttcct gacctaaggt cttgtgagct
     6361 tgtatttttt ctatttaagc agtgctttct cttggactgg cttgactcat ggcattctac
     6421 acgttattgc tggtctaaat gtgattttgc caagcttctt caggacctat aattttgctt
     6481 gacttgtagc caaacacaag taaaatgatt aagcaacaaa tgtatttgtg aagcttggtt
     6541 tttaggttgt tgtgttgtgt gtgcttgtgc tctataataa tactatccag gggctggaga
     6601 ggtggctcgg agttcaagag cacagactgc tcttccagaa gtcctgagtt caattcccag
     6661 caaccacatg gtggctcaca accatctgta atgggatctg atgccctctt ctggtgtgtc
     6721 tgaagaccac aagtgtattc acattaaata aataaatcct ccttcttctt cttttttttt
     6781 tttttaaaga gaatactgtc tccagtagaa tttactgaag taatgaaata ctttgtgttt
     6841 gttccaatat ggtagccaat aatcaaatta ctctttaagc actggaaatg ttaccaagga
     6901 actaattttt atttgaagtg taactgtgga cagaggagcc ataactgcag acttgtggga
     6961 tacagaagac caatgcagac tttaatgtct tttctcttac actaagcaat aaagaaataa
     7021 aaattgaact tctagtatcc tatttgttaa actgctagct ttacttaact tttgtgcttc
     7081 atctatacaa agctgaaagc taagtctgca gccattacta aacatgaaag caagtaatga
     7141 taattttgga tttcaaaaat gtagggccag agtttagcca gccagtggtg gtgcttgcct
     7201 ttatgccttt aatcccagca ctctggaggc agagacaggc agatctctga gtttgagccc
     7261 agcctggtct acacatcaag ttctatctag gatagccagg aatacacaca gaaaccctgt
     7321 tggggagggg ggctctgaga tttcataaaa ttataattga agcattccct aatgagccac
     7381 tatggatgtg gctaaatccg tctacctttc tgatgagatt tgggtattat tttttctgtc
     7441 tctgctgttg gttgggtctt ttgacactgt gggctttctt taaagcctcc ttccctgcca
     7501 tgtggtctct tgtttgctac taacttccca tggcttaaat ggcatggctt tttgccttct
     7561 aagggcagct gctgagattt gcagcctgat ttccagggtg gggttgggaa atctttcaaa
     7621 cactaaaatt gtcctttaat ttttttttta aaaaatgggt tatataataa acctcataaa
     7681 atagttatga ggagtgaggt ggactaatat taaatgagtc cctcccctat aaaagagcta
     7741 ttaaggcttt ttgtcttata cttaactttt tttttaaatg tggtatcttt agaaccaagg
     7801 gtcttagagt tttagtatac agaaactgtt gcatcgctta atcagatttt ctagtttcaa
     7861 atccagagaa tccaaattct tcacagccaa agtcaaatta agaatttctg acttttaatg
     7921 ttaatttgct tactgtgaat ataaaaatga tagcttttcc tgaggcaggg tctcactatg
     7981 tatctctgcc tgatctgcaa caagatatgt agactaaagt tctgcctgct tttgtctcct
     8041 gaatactaag gttaaaatgt agtaatactt ttggaacttg caggtcagat tcttttatag
     8101 gggacacact aagggagctt gggtgatagt tggtaaaatg tgtttcaagt gatgaaaact
     8161 tgaattatta tcaccgcaac ctacttttta aaaaaaaaag ccaggcctgt tagagcatgc
     8221 ttaagggatc cctaggactt gctgagcaca caagagtagt tacttggcag gctcctggtg
     8281 agagcatatt tcaaaaaaca aggcagacaa ccaagaaact acagttaagg ttacctgtct
     8341 ttaaaccatc tgcatataca cagggatatt aaaatattcc aaataatatt tcattcaagt
     8401 tttcccccat caaattggga catggatttc tccggtgaat aggcagagtt ggaaactaaa
     8461 caaatgttgg ttttgtgatt tgtgaaattg ttttcaagtg atagttaaag cccatgagat
     8521 acagaacaaa gctgctattt cgaggtctct tggtttatac tcagaagcac ttctttgggt
     8581 ttccctgcac tatcctgatc atgtgctagg cctaccttag gctgattgtt gttcaaataa
     8641 acttaagttt cctgtcaggt gatgtcatat gatttcatat atcaaggcaa aacatgttat
     8701 atatgttaaa catttgtact taatgtgaaa gttaggtctt tgtgggtttt gatttttaat
     8761 tttcaaaacc tgagctaaat aagtcatttt tacatgtctt acatttggtg gaattgtata
     8821 attgtggttt gcaggcaaga ctctctgacc tagtaaccct acctatagag cactttgctg
     8881 ggtcacaagt ctaggagtca agcatttcac cttgaagttg agacgttttg ttagtgtata
     8941 ctagtttata tgttggagga catgtttatc cagaagatat tcaggactat ttttgactgg
     9001 gctaaggaat tgattctgat tagcactgtt agtgagcatt gagtggcctt taggcttgaa
     9061 ttggagtcac ttgtatatct caaataatgc tggccttttt taaaaagccc ttgttcttta
     9121 tcaccctgtt ttctacataa tttttgttca aagaaatact tgtttggatc tccttttgac
     9181 aacaatagca tgttttcaag ccatattttt tttccttttt tttttttttt ttggtttttc
     9241 gagacagggt ttctctgtat agccctggct gtcctggaac tcactttgta gaccaggctg
     9301 gcctcgaact cagaaatccg cctgcctctg cctcctgagt gccgggatta aaggcgtgca
     9361 ccaccacgcc tggctaagtt ggatattttg tatataacta taaccaatac taactccact
     9421 gggtggattt ttaattcagt cagtagtctt aagtggtctt tattggccct tcattaaaat
     9481 ctactgttca ctctaacaga ggctgttggt actagtggca cttaagcaac ttcctacgga
     9541 tatactagca gattaagggt cagggataga aactagtcta gcgttttgta tacctaccag
     9601 ctttatacta ccttgttctg atagaaatat ttcaggacat ctagcttatc g
//