LOCUS HM588138 9651 bp DNA linear SYN 08-NOV-2010 DEFINITION Targeting vector R26-Stop-HA3-ICA512-CCF, partial sequence. ACCESSION HM588138 VERSION HM588138.1 KEYWORDS . SOURCE Targeting vector R26-Stop-HA3-ICA512-CCF ORGANISM Targeting vector R26-Stop-HA3-ICA512-CCF other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9651) AUTHORS Liu,Y., Suckale,J., Masjkur,J., Magro,M.G., Steffen,A., Anastassiadis,K. and Solimena,M. TITLE Tamoxifen-Independent Recombination in the RIP-CreER Mouse JOURNAL PLoS ONE 5 (10), E13533 (2010) PUBMED 21063464 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 9651) AUTHORS Suckale,J. TITLE Direct Submission JOURNAL Submitted (23-JUN-2010) Paul Langerhans Institute, Dresden University of Technology, Fiedlerstr. 42, Dresden, Saxony 01307, Germany FEATURES Location/Qualifiers source 1..9651 /organism="Targeting vector R26-Stop-HA3-ICA512-CCF" /mol_type="other DNA" /db_xref="taxon:885264" /note="targeting vector for homologous recombination into endogenous locus (mouse and foreign sequences); Cre-activated expression of Ptprn (ICA512) cleaved cytosolic fragment from the ROSA26 locus" gene <1..>1090 /gene="Rosa26" misc_feature <1..>1090 /gene="Rosa26" /note="5' homology arm" regulatory 1091..1208 /regulatory_class="other" /note="splice acceptor" misc_recomb 1215..1248 /note="loxP" CDS 1791..2594 /codon_start=1 /transl_table=11 /product="neomycin resistance" /protein_id="ADN52072.1" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD LDEEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 3152..3343 /regulatory_class="polyA_signal_sequence" regulatory 3406..3597 /regulatory_class="polyA_signal_sequence" regulatory 3660..3851 /regulatory_class="polyA_signal_sequence" misc_recomb 3884..3917 /note="loxP" CDS 3943..5019 /note="3xHA-ICA512 cleaved cytosolic fragment" /codon_start=1 /transl_table=11 /product="PTPRN-CCF" /protein_id="ADN52073.1" /translation="MARYPYDVPDYAGYPYDVPDYAGSYPYDVPDYASRDLSQFSDAA QASPSSHSSTPSWCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEP NTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYI ATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNL VSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKC YRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRS KDQFEFALTAVAEEVNAILKALPQ" gene <5308..>9651 /gene="Rosa26" misc_feature 5308..9651 /gene="Rosa26" /note="3' homology arm" BASE COUNT 2305 a 2288 c 2365 g 2693 t ORIGIN 1 gccagcggca ggccctccga gcgtggtgga gccgttctgt gagacagccg ggtacgagtc 61 gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc ggtccgccct gcagcaaccg 121 gagggggagg gagaagggag cggaaaagtc tccaccggac gcggccatgg ctcggggggg 181 ggggggcagc ggaggagcgc ttccggccga cgtctcgtcg ctgattggct tcttttcctc 241 ccgccgtgtg tgaaaacaca aatggcgtgt tttggttggc gtaaggcgcc tgtcagttaa 301 cggcagccgg agtgcgcagc cgccggcagc ctcgctctgc ccactgggtg gggcgggagg 361 taggtggggt gaggcgagct ggacgtgcgg gcgcggtcgg cctctggcgg ggcgggggag 421 gggagggagg gtcagcgaaa gtagctcgcg cgcgagcggc cgcccaccct ccccttcctc 481 tgggggagtc gttttacccg ccgccggccg ggcctcgtcg tctgattggc tctcggggcc 541 cagaaaactg gcccttgcca ttggctcgtg ttcgtgcaag ttgagtccat ccgccggcca 601 gcgggggcgg cgaggaggcg ctcccaggtt ccggccctcc cctcggcccc gcgccgcaga 661 gtctggccgc gcgcccctgc gcaacgtggc aggaagcgcg cgctgggggc ggggacgggc 721 agtagggctg agcggctgcg gggcgggtgc aagcacgttt ccgacttgag ttgcctcaag 781 aggggcgtgc tgagccagac ctccatcgcg cactccgggg agtggaggga aggagcgagg 841 gctcagttgg gctgttttgg aggcaggaag cacttgctct cccaaagtcg ctctgagttg 901 ttatcagtaa gggagctgca gtggagtagg cggggagaag gccgcaccct tctccggagg 961 ggggagggga gtgttgcaat acctttctgg gagttctctg ctgcctcctg gcttctgagg 1021 accgccctgg gcctgggaga atcccttgcc ccctcttccc ctcgtgatct gcaactccag 1081 tctttctagc gtgacctgca cgtctagggc gcagtagtcc agggtttcct tgatgatgtc 1141 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 1201 tttccagtcc gcggataact tcgtatagca tacattatac gaagttatta ggtccctcga 1261 cctgcaggaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc 1321 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc 1381 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc 1441 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg 1501 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg 1561 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc 1621 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc 1681 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc 1741 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagccaat atgggatcgg 1801 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 1861 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 1921 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 1981 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 2041 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 2101 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 2161 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 2221 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 2281 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 2341 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 2401 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 2461 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 2521 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 2581 acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct attaaacaat 2641 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca 2701 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta 2761 gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag 2821 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc 2881 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt 2941 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca 3001 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat 3061 cagaagcttg cagatctgcg actctagagg atctgcgact ctagaggatc ataatcagcc 3121 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 3181 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 3241 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 3301 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc gactctagag 3361 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 3421 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 3481 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 3541 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 3601 ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta 3661 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 3721 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 3781 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 3841 tatcatgtct ggatccccat caagctgatc cggaaccctt aatataactt cgtatagcat 3901 acattatacg aagttatagc tagattcgaa cccggtgcca ccatggcgag atacccatac 3961 gatgtccctg actatgcggg ctatccctat gacgtcccgg actatgcagg atcctaccca 4021 tatgacgttc cagattacgc ttcaagagat ctctcccagt tcagcgacgc agcccaggcc 4081 agccccagct cccacagcag caccccgtcc tggtgcgagg agccggccca agccaacatg 4141 gacatctcca cgggacacat gattctggca tacatggagg atcacctgcg gaaccgggac 4201 cgccttgcca aggagtggca ggccctctgt gcctaccaag cagagccaaa cacctgtgcc 4261 accgcgcagg gggagggcaa catcaaaaag aaccggcatc ctgacttcct gccctatgac 4321 catgcccgca taaaactgaa ggtggagagc agcccttctc ggagcgatta catcaacgcc 4381 agccccatta ttgagcatga ccctcggatg ccagcctaca tagccacgca gggcccgctg 4441 tcccatacca tcgcagactt ctggcagatg gtgtgggaga gcggctgcac cgtcatcgtc 4501 atgctgaccc cgctggtgga ggatggtgtc aagcagtgtg accgctactg gccagatgag 4561 ggtgcctccc tctaccacgt atatgaggtg aacctggtgt cggagcacat ctggtgcgag 4621 gactttctgg tgcggagctt ctacctgaag aacgtgcaga cccaggagac gcgcacgctc 4681 acgcagttcc acttcctcag ctggccggca gagggcacac cggcctccac gcggcccctg 4741 ctggacttcc gcaggaaggt gaacaagtgc taccggggcc gctcctgccc catcatcgtg 4801 cactgcagtg atggtgcggg gaggaccggc acctacatcc tcatcgacat ggtcctgaac 4861 cgcatggcaa aaggagtgaa ggagattgac atcgctgcca ccctggagca tgtccgtgac 4921 cagcggcctg gccttgtccg ctctaaggac cagtttgaat ttgccctgac agccgtggcg 4981 gaggaagtga atgccatcct caaggccctg ccccagtgac gaattggggg gaggctaact 5041 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag 5101 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg 5161 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt 5221 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg 5281 gcaggccctg ccatagacgc gtgctagaag atgggcggga gtcttctggg caggcttaaa 5341 ggctaacctg gtgtgtgggc gttgtcctgc aggggaattg aacaggtgta aaattggagg 5401 gacaagactt cccacagatt ttcggttttg tcgggaagtt ttttaatagg ggcaaataag 5461 gaaaatggga ggataggtag tcatctgggg ttttatgcag caaaactaca ggttattatt 5521 gcttgtgatc cgcctcggag tattttccat cgaggtagat taaagacatg ctcacccgag 5581 ttttatactc tcctgcttga gatccttact acagtatgaa attacagtgt cgcgagttag 5641 actatgtaag cagaatttta atcattttta aagagcccag tacttcatat ccatttctcc 5701 cgctccttct gcagccttat caaaaggtat tttagaacac tcattttagc cccattttca 5761 tttattatac tggcttatcc aacccctaga cagagcattg gcattttccc tttcctgatc 5821 ttagaagtct gatgactcat gaaaccagac agattagtta catacaccac aaatcgaggc 5881 tgtagctggg gcctcaacac tgcagttctt ttataactcc ttagtacact ttttgttgat 5941 cctttgcctt gatccttaat tttcagtgtc tatcacctct cccgtcaggt ggtgttccac 6001 atttgggcct attctcagtc cagggagttt tacaacaata gatgtattga gaatccaacc 6061 taaagcttaa ctttccactc ccatgaatgc ctctctcctt tttctccatt tataaactga 6121 gctattaacc attaatggtt tccaggtgga tgtctcctcc cccaatatta cctgatgtat 6181 cttacatatt gccaggctga tattttaaga cattaaaagg tatatttcat tattgagcca 6241 catggtattg attactgctt actaaaattt tgtcattgta cacatctgta aaaggtggtt 6301 ccttttggaa tgcaaagttc aggtgtttgt tgtctttcct gacctaaggt cttgtgagct 6361 tgtatttttt ctatttaagc agtgctttct cttggactgg cttgactcat ggcattctac 6421 acgttattgc tggtctaaat gtgattttgc caagcttctt caggacctat aattttgctt 6481 gacttgtagc caaacacaag taaaatgatt aagcaacaaa tgtatttgtg aagcttggtt 6541 tttaggttgt tgtgttgtgt gtgcttgtgc tctataataa tactatccag gggctggaga 6601 ggtggctcgg agttcaagag cacagactgc tcttccagaa gtcctgagtt caattcccag 6661 caaccacatg gtggctcaca accatctgta atgggatctg atgccctctt ctggtgtgtc 6721 tgaagaccac aagtgtattc acattaaata aataaatcct ccttcttctt cttttttttt 6781 tttttaaaga gaatactgtc tccagtagaa tttactgaag taatgaaata ctttgtgttt 6841 gttccaatat ggtagccaat aatcaaatta ctctttaagc actggaaatg ttaccaagga 6901 actaattttt atttgaagtg taactgtgga cagaggagcc ataactgcag acttgtggga 6961 tacagaagac caatgcagac tttaatgtct tttctcttac actaagcaat aaagaaataa 7021 aaattgaact tctagtatcc tatttgttaa actgctagct ttacttaact tttgtgcttc 7081 atctatacaa agctgaaagc taagtctgca gccattacta aacatgaaag caagtaatga 7141 taattttgga tttcaaaaat gtagggccag agtttagcca gccagtggtg gtgcttgcct 7201 ttatgccttt aatcccagca ctctggaggc agagacaggc agatctctga gtttgagccc 7261 agcctggtct acacatcaag ttctatctag gatagccagg aatacacaca gaaaccctgt 7321 tggggagggg ggctctgaga tttcataaaa ttataattga agcattccct aatgagccac 7381 tatggatgtg gctaaatccg tctacctttc tgatgagatt tgggtattat tttttctgtc 7441 tctgctgttg gttgggtctt ttgacactgt gggctttctt taaagcctcc ttccctgcca 7501 tgtggtctct tgtttgctac taacttccca tggcttaaat ggcatggctt tttgccttct 7561 aagggcagct gctgagattt gcagcctgat ttccagggtg gggttgggaa atctttcaaa 7621 cactaaaatt gtcctttaat ttttttttta aaaaatgggt tatataataa acctcataaa 7681 atagttatga ggagtgaggt ggactaatat taaatgagtc cctcccctat aaaagagcta 7741 ttaaggcttt ttgtcttata cttaactttt tttttaaatg tggtatcttt agaaccaagg 7801 gtcttagagt tttagtatac agaaactgtt gcatcgctta atcagatttt ctagtttcaa 7861 atccagagaa tccaaattct tcacagccaa agtcaaatta agaatttctg acttttaatg 7921 ttaatttgct tactgtgaat ataaaaatga tagcttttcc tgaggcaggg tctcactatg 7981 tatctctgcc tgatctgcaa caagatatgt agactaaagt tctgcctgct tttgtctcct 8041 gaatactaag gttaaaatgt agtaatactt ttggaacttg caggtcagat tcttttatag 8101 gggacacact aagggagctt gggtgatagt tggtaaaatg tgtttcaagt gatgaaaact 8161 tgaattatta tcaccgcaac ctacttttta aaaaaaaaag ccaggcctgt tagagcatgc 8221 ttaagggatc cctaggactt gctgagcaca caagagtagt tacttggcag gctcctggtg 8281 agagcatatt tcaaaaaaca aggcagacaa ccaagaaact acagttaagg ttacctgtct 8341 ttaaaccatc tgcatataca cagggatatt aaaatattcc aaataatatt tcattcaagt 8401 tttcccccat caaattggga catggatttc tccggtgaat aggcagagtt ggaaactaaa 8461 caaatgttgg ttttgtgatt tgtgaaattg ttttcaagtg atagttaaag cccatgagat 8521 acagaacaaa gctgctattt cgaggtctct tggtttatac tcagaagcac ttctttgggt 8581 ttccctgcac tatcctgatc atgtgctagg cctaccttag gctgattgtt gttcaaataa 8641 acttaagttt cctgtcaggt gatgtcatat gatttcatat atcaaggcaa aacatgttat 8701 atatgttaaa catttgtact taatgtgaaa gttaggtctt tgtgggtttt gatttttaat 8761 tttcaaaacc tgagctaaat aagtcatttt tacatgtctt acatttggtg gaattgtata 8821 attgtggttt gcaggcaaga ctctctgacc tagtaaccct acctatagag cactttgctg 8881 ggtcacaagt ctaggagtca agcatttcac cttgaagttg agacgttttg ttagtgtata 8941 ctagtttata tgttggagga catgtttatc cagaagatat tcaggactat ttttgactgg 9001 gctaaggaat tgattctgat tagcactgtt agtgagcatt gagtggcctt taggcttgaa 9061 ttggagtcac ttgtatatct caaataatgc tggccttttt taaaaagccc ttgttcttta 9121 tcaccctgtt ttctacataa tttttgttca aagaaatact tgtttggatc tccttttgac 9181 aacaatagca tgttttcaag ccatattttt tttccttttt tttttttttt ttggtttttc 9241 gagacagggt ttctctgtat agccctggct gtcctggaac tcactttgta gaccaggctg 9301 gcctcgaact cagaaatccg cctgcctctg cctcctgagt gccgggatta aaggcgtgca 9361 ccaccacgcc tggctaagtt ggatattttg tatataacta taaccaatac taactccact 9421 gggtggattt ttaattcagt cagtagtctt aagtggtctt tattggccct tcattaaaat 9481 ctactgttca ctctaacaga ggctgttggt actagtggca cttaagcaac ttcctacgga 9541 tatactagca gattaagggt cagggataga aactagtcta gcgttttgta tacctaccag 9601 ctttatacta ccttgttctg atagaaatat ttcaggacat ctagcttatc g //