LOCUS FR872379 8194 bp DNA circular SYN 06-MAR-2012
DEFINITION Shuttle vector pAZILcos.
ACCESSION FR872379
VERSION FR872379.1
KEYWORDS .
SOURCE Shuttle vector pAZILcos
ORGANISM Shuttle vector pAZILcos
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8194)
AUTHORS Kojic M.
JOURNAL Submitted (27-MAY-2011) to the INSDC. Kojic M., Imgge, Lmgim,
Vojvode Stepe 444a, Belgrade, Serbia, 11010, SERBIA AND MONTENEGRO.
REFERENCE 2
AUTHORS Kojic M., Jovcic B., Strahinic I., Begovic J., Lozo J.,
Veljovic K., Topisirovic L.
TITLE Cloning and expression of a novel lactococcal aggregation factor
from Lactococcus lactis subsp. lactis BGKP1
JOURNAL BMC Microbiol. 11, 265-265(2011).
PUBMED 22182285
FEATURES Location/Qualifiers
source 1..8194
/organism="Shuttle vector pAZILcos"
/lab_host="Lactococcus lactis"
/mol_type="other DNA"
/db_xref="taxon:1041405"
CDS 890..1396
/transl_table=11
/gene="lacZ"
/product="beta-galactosidase"
/protein_id="CCB84606.1"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADT
VVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRI
IFDGVPIG"
CDS complement(2555..4045)
/transl_table=11
/gene="repE"
/product="replication protein"
/function="replication"
/protein_id="CCB84607.1"
/translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
CDS complement(4088..4387)
/transl_table=11
/gene="repD"
/product="replication protein"
/function="replication"
/protein_id="CCB84608.1"
/translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
CDS complement(4754..4987)
/transl_table=11
/gene="copS like"
/product="copy number repressor"
/protein_id="CCB84609.1"
/translation="MLAVPQELEMSRSNYSRIESGKSDPTIKTLEQIAKLTNSTLVVD
LIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
CDS complement(5629..6366)
/transl_table=11
/gene="ermB"
/product="erythromicin ribosomal methylase B"
/protein_id="CCB84610.1"
/translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT 2231 a 1791 c 1532 g 2640 t
ORIGIN
1 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
61 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt
121 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
181 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
241 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
301 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
361 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
421 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
481 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
541 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt
601 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
661 ttgacagctt atcatcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
721 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
781 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
841 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
901 tacgaattcg agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt
961 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
1021 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
1081 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
1141 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
1201 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta
1261 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
1321 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
1381 cgttcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaatgcgaa ttttaacaaa
1441 ataacgccat gagcggcctc atttcttatt ctgagttaca acagtccgca ccgctgccgg
1501 tagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
1561 tatcatgcaa ctcgtaggac aggtgccggc agcgcccaac agtcccccgg ccacggggcc
1621 tgccaccata cccacgccga aacaagcgcc ctgcaccatt atgttccgga tctgcatcgc
1681 agatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctaaccgttt
1741 ttatcagctc tgggaggcag aataaatgat catatcgtca attattacct ccacggggag
1801 agcctgagca aactggcctc aggcatttga gaagcacacg gtcacactgc ttccggtagt
1861 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
1921 gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt
1981 caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
2041 ccctgccact catcgcagta attctattta atcactttga ctagcaaata ctaacaacaa
2101 gacacacaca ccaaaaatca aaaattcact acttttagtt aaaaaccacg taaccacaag
2161 aactaatcca atccatgtaa tcgggttctt caaatatttc tccaagattt tcctcctcta
2221 atatgctcaa cttaaatgac ctattcaata aatctattat gctgctaaat agtttatagg
2281 acaaataagt atactctaat gacctataaa agatagaaaa ttaaaaaatc aagtgttcgc
2341 ttcgctctca ctgcccctcg acgttttagt agcctttccc tcacttcgtt cagtccaagc
2401 caactaaaag ttttcgggct actctctcct tctcccccta ataattaatt aaaatcttac
2461 tctgtatatt tctgctaatc attcgctaaa cagcaaagaa aaaacaaaca cgtatcatag
2521 atataaatgt aatggcatag tgcgggtttt attttcagcc tgtatcatag ctaaacaaat
2581 cgagttgtgt gtccgtttta gggcgttctg ctagcttgtt taaagtctct tgaatgaatg
2641 tatgctctaa gtcaaaagaa tttgtcagcg cctttatata gctttctttt tcttcttttt
2701 ttactttaat gatcgatagc aacaatgatt taacactagc aagttgaatg ccaccatttc
2761 ttcctggttt aatcttaaag aaaatttcct gattcgcctt cagtaccttc agcaatttat
2821 ctaatgtccg ttcaggaatg cctagcactt ctctaatctc ttttttggtc gtcactaaat
2881 aaggcttgta tacatcgctt ttttcgctaa tataagccat taaatcttct ttccattctg
2941 acaaatgaac acgttgacgt tcgcttcttt ttttcttgaa tttaaaccac ccttgacgga
3001 caaataaatc tttactggtt aaatcacttg atacccaagc tttgcaaaga atggtaatgt
3061 attccctatt agccccttga tagttttctg aataggcact tctaacaatt ttgattactt
3121 ctttttcttc taagggttga tctaatcgat tattaaactc aaacatatta tattcgcacg
3181 tttcgattga atagcctgaa ctaaagtagg ctaaagagag ggtaaacatg acgttattac
3241 gccctattaa acccttttct cctgaaaatt tcgtttcgtg caataagaga ttaaaccagg
3301 gttcatctac ttgttttttg ccttctgtac cgcttaaaac cgttagactt gaacgagtaa
3361 agcccttatt atctgtttgt ttgaaagacc aatcttgcca ttctttgaaa gaataacggt
3421 aattaggatc aaaaaattct acattgtccg ttcttggtat gcgagcaata ccaaaatgat
3481 tacacgttag atcaactggc aaagactttc caaaatattc tcggatattt tgcgaaatta
3541 ttttggctgc tttgacagat ttaaattctg attttgaagt cacatagact ggcgtttcta
3601 aaacaaaata tgcttgataa cctttatcag atttgataat catagtaggc ataaaaccta
3661 aatcaatagc ggttgttaaa atatcgcttg ctgaaatagt ttcttttgcc gtgtgaatat
3721 caaaatcaat aaagaaggta ttgatttgtc ttaaattgtt ttcagaatgt cctttcgtgt
3781 atgaacggtt ttcgtctgca tacgttccat aacgataaac gttgggtgtc caatgtgtaa
3841 atgtatcttg attttcttga atcgcttcct cggaagtcag aacaacacca cgaccgccaa
3901 tcatgcttga ttttgagcga tacgcaaaaa tagccccttt gcttttacct ggcttggtag
3961 tgattgagcg aattttacta tttttaaatt tgtactttaa caagccgtca tgaagcacag
4021 tttctacaac aaaagggata ttcattcagc tgttctcctt tcctataaat cctataaaat
4081 aggttgttta attaacttgg tttgcttttt cattcaactg tttcaatatt gcatgttttg
4141 aaaaagattt ttttccttta taagtcaatt tttttccact aatcgaataa attattttgt
4201 tattttctat taacttatat atataatctt ccccctccga agaaaaatac ttatctgatt
4261 ttgtttctaa gtagatattt ctcttttcta actctttctt aaacgtttct agtgtataga
4321 tatttgctaa ttttcttatc tccaataaac tattttttat ataagtttta cattcatcat
4381 gattcataca aactccacct tctataaatg aatacaaaaa aagcaatcaa acgatttccg
4441 attgattgct taacaattct taaattcagt agcttagata cttgaaaact ctctgatttc
4501 cctatataat gatagtacgg ttatataccg tcttcaaaca aagttaatta aataacttct
4561 tacgagggaa gagttcatct gactaactga taagcgttgg tttggcaatc ttatcgggct
4621 atgcatttat aaaatgtcgt caaacatttt ataaatgtgt catggctctt ttttcgtttc
4681 tattcagttc gttgtttcgt tatatctagt ataccgcttt taaaaaaaaa taagcaacga
4741 tttcgtgcat tattcacacg aagtcattgc ttttttcttc ttccatttct aaatccaatg
4801 ttacttgttc tgattctgtt tctggctctg gttctgttgg ctcatttggg attaaatcca
4861 ctactagcgt tgagttagtt aactttgcaa tttgttctag tgtttttatg gttggatctg
4921 attttcctga ttctattcgt gaataatttg atctactcat ttctaattct tggggtaccg
4981 ccagcatttc ggaaaaaaac cacgctaagg attttttcta taaaaagagc cgttatatta
5041 agaataaaac ggctctttta tacgtaaagg acgtaaattc atttgcccag tgtcatgtaa
5101 tccttcaaat ttgtattctc caagaaaatt gatatgttcc catcctaacg gccacgcata
5161 tggcattaaa tcttctctaa attctcctct tgcttttaat tcttctacgg ctttttccat
5221 atatacagtg ttccacacac ttatagcgtt aataattatg tttagtgcac tagctctttg
5281 taactggtct tggagagcac gttctctaaa ttctccacgt tgtccaaaaa atatagttct
5341 agctaatgca ttgattgctt ctcctttatt taaacctttt tgaacccgtc tccttacggc
5401 tttattagat atgtaatcca gcgtaaagag ggttttctcg attcgtccca tttctccaag
5461 tgctgttgcg agtttatttt gtcttgcata tgatccgagc ttccccatga taagagcgct
5521 agggacctct ttagctcctt ggaagctgtc agtagtatac ctaataattt atctacattc
5581 cctttagtaa cgtgtaactt tccaaattta caaaagcgac tcatagaatt atttcctccc
5641 gttaaataat agataactat taaaaataga caatacttgc tcataagtaa cggtacttaa
5701 attgtttact ttggcgtgtt tcattgcttg atgaaactga tttttagtaa acagttgacg
5761 atattctcga ttgacccatt ttgaaacaaa gtacgtatat agcttccaat atttatctgg
5821 aacatctgtg gtatggcggg taagttttat taagacactg tttacttttg gtttaggatg
5881 aaagcattcc gctggcagct taagcaattg ctgaatcgag acttgagtgt gcaagagcaa
5941 ccctagtgtt cggtgaatat ccaaggtacg cttgtagaat ccttcttcaa caatcagata
6001 gatgtcagac gcatggcttt caaaaaccac ttttttaata atttgtgtgc ttaaatggta
6061 aggaatactc ccaacaattt tatacctctg tttgttaggg aattgaaact gtagaatatc
6121 ttggtgaatt aaagtgacac gagtattcag ttttaatttt tctgacgata agttgaatag
6181 atgactgtct aattcaatag acgttacctg tttacttatt ttagccagtt tcgtcgttaa
6241 atgcccttta cctgttccaa tttcgtaaac ggtatcggtt tcttttaaat tcaattgttt
6301 tattatttgg ttgagtactt tttcactcgt taaaaagttt tgagaatatt ttatattttt
6361 gttcatgtaa tcactccttc ttaattacaa atttttagca tctaatttaa cttcaattcc
6421 tattatacaa aattttaaga tactgcacta tcaacacact cttaagtttg cttctaagtc
6481 ttatttccat aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat
6541 ggtgcaagtc agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga
6601 ttggtgtatc atttcgtttt tcttttgcgc ttcttgataa aagggatagt aattcattcc
6661 tggttgcaaa ttttgaaaac cgctacggat cacatctttt tctaaactat tgatccatag
6721 tcttttatac gttttatctt tagaaaaggc atttgcttta tgaatgatcg accaggcaat
6781 gttttcgcct tctctgtcgc tatctgttgc gacaatgatt gtatttgctt gttttaaaag
6841 ttcagcaaca attttaaact gctttttttt atctgttgcc acttcaaaat cgtatcgatt
6901 ctaggcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata
6961 caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
7021 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
7081 cagggaagtg agagggccgc ggaagctagc ttcgaggaac tttcggcggc tttgctgtgc
7141 gacaggctca cgtctaaaag gaaataaatc atgggtcata aaattatcac gttgtccggc
7201 gcggcgacgg atgttctgta tgcgctgttt ttccgtggcg cgttgctgtc tggtgatctg
7261 ccttctaaat ctggcacagc cgaattgcgc gagcttggtt ttgctgaaac cagacacaca
7321 gcaactgaat accagaaaga aaatcacttt acctttctga catcagaagg gcagaaattt
7381 gccgttgaac acctggtcaa tacgcgtttt ggtgagcagc aatattgcgc ttcgatgacg
7441 cttggcgttg agattgatac ctctgctgca caaaaggcaa tcgacgagct ggaccagcgc
7501 attcgtgaca ccgtctcctt cgaacttatt cgcaatggag tgtcattcat caaggacgcc
7561 gctatcgcaa atggtgctat ccacgcagcg gcaatcgaaa cacctcagcc ggtgaccaat
7621 atctacaaca tcagccttgg tatccagcgt gatgagccag cgcagaacaa ggtaaccgtc
7681 agtgccgata agttcaaagt taaacctggt gttgatacca acattgaaac gttgatcgaa
7741 aacgcgctga aaaacgctgc tgaatgtgcg gcgctggatg tcacaaagca aatggcagca
7801 gacaagaaag cgatggatga actggcttcc tatgtccgca cggccatcat gatggaatgt
7861 ttccccggtg gtgttatctg gcagcagtgc cgtcgatagt atgcaattga taattattat
7921 catttgcggg tcctttccgg cgatccgcct tgttacgggg cggcgacctc gcgggttttc
7981 gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca taacttaatg
8041 tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa gcgagctttt
8101 tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg aagtcaacaa
8161 aaagcagagc ttatcgatga tccgcggcaa agcc
//