LOCUS EU310372 891 bp DNA linear BCT 26-JAN-2011
DEFINITION Pseudomonas fluorescens strain JCM5963 lipase gene, complete cds.
ACCESSION EU310372
VERSION EU310372.1
KEYWORDS .
SOURCE Pseudomonas fluorescens
ORGANISM Pseudomonas fluorescens
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 891)
AUTHORS Zhang,A., Cao,R., Diao,N., Xie,G., Gao,G. and Gao,S.
TITLE Cloning, expression and characterization of an organic solvent
tolerant lipase from Pseudomonas fluorescens JCM5963
JOURNAL J. Mol. Catal., B Enzym. 56 (2-3), 78-84 (2009)
REFERENCE 2 (bases 1 to 891)
AUTHORS Zhang,A., Cao,S., Gao,R. and Diao,N.
TITLE Direct Submission
JOURNAL Submitted (29-NOV-2007) Key Laboratory for Molecular Enzymology and
Engineering of Ministry of Education, Jilin University of China,
2519 Jiefang Road, Changchun, Jilin 130021, China
FEATURES Location/Qualifiers
source 1..891
/organism="Pseudomonas fluorescens"
/mol_type="genomic DNA"
/strain="JCM5963"
/isolation_source="pre-filter tanks"
/type_material="type strain of Pseudomonas fluorescens"
/db_xref="taxon:294"
/country="United Kingdom: England"
/note="type strain of Pseudomonas fluorescens"
CDS 1..891
/EC_number="3.1.1.3"
/note="alpha,beta hydrolyase fold; high lipidlysis
activity"
/codon_start=1
/transl_table=11
/product="lipase"
/protein_id="ABY26520.1"
/translation="MSQELATRYPLVLVPGMLGFVRLLLYPYWYGIIPALRRGGAQVI
AVQVSPLNSSEVRGEQLLAQIQRIMAETGAARVNLIGHSQGALTARYAAARRPDWVAS
VTSVAGPNHGSELADYLQRHSPAHSLRGRVLSLLLRGISSLMRLLETGYRGPKQPVDI
HASHQSLTTEGVALFNRQYPQGLPQEWGGQGPAQVDGVHYYSWSGILQPGKTNRGRNL
FDGTNRSCRLFARTFVREAGQCDGMVGRYSSHLGQVIGDDYPLDHFDIVNQSLGLVGK
GAEPIRLFVEHARRLKAAGL"
BASE COUNT 136 a 299 c 302 g 154 t
ORIGIN
1 atgtcccaag agcttgccac ccgttacccc ctggtcctgg tgcccggcat gctcggcttt
61 gtccgcctgc tgctgtaccc ctactggtac gggatcattc cggccttgcg ccggggcggg
121 gcgcaggtga tcgcggtgca ggtctcgccg ctcaattcca gcgaggtgcg cggtgagcag
181 ttgctggcgc agatccagcg gatcatggcc gagaccggcg ccgcccgagt caacctgatc
241 ggccacagcc agggcgcgct caccgcgcgc tacgccgccg ccaggcgccc ggactgggtg
301 gcctcggtaa cgtcggtggc ggggcccaac catggctccg agctggcgga ttatctgcag
361 cggcattcac ccgcccatag cctgcggggg cgagtgctga gcctgctgct gagggggatt
421 tcaagcctga tgcggttgct ggaaaccggc tatcgcggac cgaagcagcc ggtggatatc
481 catgcttcgc accagtccct gaccaccgag ggcgtggccc tgttcaaccg ccagtacccc
541 cagggattgc cgcaggaatg gggcgggcaa gggccggcgc aggtcgacgg tgtgcactac
601 tactcctggt ccggcatttt gcagccgggc aagaccaacc gcgggcgtaa cctgttcgac
661 ggcaccaacc gcagttgccg gctgttcgcc cgcaccttcg tccgtgaagc cggccagtgc
721 gacggcatgg tggggcgcta cagctcgcac ctggggcagg tcattggcga tgattacccg
781 ctggaccatt tcgatatcgt caaccagtcc ctggggctgg tgggcaaggg cgcggagccg
841 atccgcctgt tcgtcgaaca tgcccggcgc ctcaaggccg ccggcctctg a
//