LOCUS EU048866 2649 bp DNA linear SYN 19-AUG-2007
DEFINITION Cloning vector pGreenII 0179, T-DNA region.
ACCESSION EU048866
VERSION EU048866.1
KEYWORDS .
SOURCE Cloning vector pGreenII 0179
ORGANISM Cloning vector pGreenII 0179
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 2649)
AUTHORS Hellens,R.P.
TITLE An update on the pGreen vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2649)
AUTHORS Hellens,R.P.
TITLE Direct Submission
JOURNAL Submitted (18-JUL-2007) Horticulture and Food Research Institute of
New Zealand (HortResearch), 120 Mt. Albert Road, Auckland, NZ 1003,
New Zealand
FEATURES Location/Qualifiers
source 1..2649
/organism="Cloning vector pGreenII 0179"
/mol_type="other DNA"
/db_xref="taxon:460115"
misc_feature 6..2618
/note="T-DNA region"
repeat_region 6..30
/note="left border repeat"
/rpt_type=direct
regulatory complement(36..258)
/regulatory_class="terminator"
/note="CaMV terminator"
gene complement(423..1448)
/gene="aphIII"
/note="AphIII"
CDS complement(423..1448)
/gene="aphIII"
/codon_start=1
/transl_table=11
/product="hygromycin resistance protein"
/protein_id="ABU39930.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory complement(1466..1807)
/regulatory_class="promoter"
/note="CaMV 35S promoter"
gene complement(1808..2245)
/gene="lacZ"
/note="LacZ"
CDS complement(1808..2245)
/gene="lacZ"
/note="blue/white selection"
/codon_start=1
/transl_table=11
/product="beta-galactosidase"
/protein_id="ABU39931.1"
/translation="MTMITPSSELTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
IPSTSRGGPVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
RTDRPSQQLRSLNGEWREIVNVNVIVPLLQKCQRYSLRRPKGY"
misc_feature 2082..2189
/note="multiple cloning site"
repeat_region 2594..2618
/note="right border repeat"
/rpt_type=direct
BASE COUNT 602 a 675 c 712 g 659 t
ORIGIN
1 agatcttggc aggatatatt gtggtgtaac gttatcgatc tggattttag tactggattt
61 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
121 agggtttctt atatgctcaa cacatgagcg aaaccctata agaaccctaa tttcccttat
181 cgggaaacta ctcacacatt atttatggag aaaatagaga gagatagatt tgtagagaga
241 gactggtgat ttcagcgtac cgaattaatt ctcccgcgac cagccgagcg agcttagcga
301 actgtggacg agaactgtgc caccaagcgt aaggccgttc tctcgcattt gccttgctag
361 gctcgcgcga gttgctggct gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta
421 ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
481 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
541 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
601 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
661 cggaggcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
721 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
781 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
841 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
901 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
961 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
1021 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
1081 gcgatcgcat ccatagcctc cgcgaccggc tgaagaacag cgggcagttc ggtttcaggc
1141 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
1201 aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
1261 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
1321 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
1381 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
1441 tttttcatag ggatcagctt gggctgtcct ctccaaatga aatgaacttc cttatataga
1501 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatgtc
1561 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
1621 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa tgatagcctt
1681 tcctttatcg caatgatggc atttgtagga gccaccttcc ttttctactg tcctttcgat
1741 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgaaatta ccctttgttg
1801 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgacatttt tggagtaggg
1861 gtacgataac attaacgttt acaatttcgc gccattcgcc attcaggctg cgcaactgtt
1921 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
1981 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
2041 cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg
2101 aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt
2161 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
2221 ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
2281 attccacaca acatacgagc cggaaghcat aaagtgtaaa gcctggggtg cctaatgagt
2341 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
2401 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
2461 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
2521 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
2581 gaacatgaag gccttgacag gatatattgg cgggtaaact aagtcgctgt atgtgtttgt
2641 ttgagatct
//