LOCUS       DQ893787                1030 bp    DNA     linear   SYN 14-JUL-2016
DEFINITION  Synthetic construct Homo sapiens clone IMAGE:100008247;
            FLH164390.01L; RZPDo839F10161D CD86 molecule (CD86) gene, encodes
            complete protein.
ACCESSION   DQ893787
VERSION     DQ893787.2
KEYWORDS    Human ORF Project; ORFeome collaboration; Gateway cloning system;
            full-length ORF without stop codon; FLEXGene.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Rolfs,A., Kelley,F., McCarron,S., Jepson,D., Shen,B., Shi,Z.,
            Hu,Y., Taycher,E., Zuo,D., Ebert,L., Hoerlein,A., Ernst,U., Korn,B.
            and LaBaer,J.
  TITLE     Cloning of human full-length CDS FLEXGene in Gateway(TM)
            recombinational vector system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Rolfs,A., Kelley,F., McCarron,S., Jepson,D., Shen,B., Shi,Z.,
            Hu,Y., Taycher,E., Zuo,D., Ebert,L., Hoerlein,A., Ernst,U., Korn,B.
            and LaBaer,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2006) Biological Chemistry and Molecular
            Pharmacology, Harvard Institute of Proteomics, 320 Charles Street,
            Cambridge, MA 02141, USA
REFERENCE   3  (bases 1 to 1030)
  AUTHORS   Rolfs,A., Kelley,F., McCarron,S., Jepson,D., Shen,B., Shi,Z.,
            Hu,Y., Taycher,E., Zuo,D., Ebert,L., Hoerlein,A., Ernst,U., Korn,B.
            and LaBaer,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2007) Biological Chemistry and Molecular
            Pharmacology, Harvard Institute of Proteomics, 320 Charles Street,
            Cambridge, MA 02141, USA
  REMARK    Sequence update by submitter
COMMENT     On Jan 22, 2007 this sequence version replaced DQ893787.1.
            This CDS clone is a part of a collection of human full-length ORF
            clones generated and verified by Harvard Institute of Proteomics
            (HIP) and Deutsches Ressourcenzentrum fuer Genomforschung GmbH
            (RZPD, Heubnerweg 6, D-14059 Berlin, Germany).  This ORF clone has
            been cloned without stop-codon (to allow fusion with C-terminal
            tag). AttB recombination sites have been added to either end of the
            ORF and directionally cloned using the Gateway cloning system into
            pDONR221. Additional sequences in the clone: 'ACC' before the 'ATG'
            (corresponding to Kozak consensus sequences).  Each clone is
            clonally isolated and full-length sequence-verified.  This clone is
            available for distribution at HIP (http://plasmid.hms.harvard.edu/)
            and RZPD (http://www.rzpd.de/products/orfclones/rzpdexpression/).
            This clone is part of the international ORFeome Collaboration
            (http://www.orfeomecollaboration.org/).
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
                     /clone="IMAGE:100008247; FLH164390.01L; RZPDo839F10161D"
                     /clone_lib="HIP_Gateway221_fusion"
                     /focus
                     /note="Vector: pDONR221; derived from parent clone
                     IMAGE:5173789 (accession BC040261.1)"
     source          23..1009
                     /organism="Homo sapiens"
                     /mol_type="other DNA"
                     /db_xref="taxon:9606"
     misc_feature    1..17
                     /note="attL1 site"
     misc_feature    18..22
                     /note="linker at the 5' end of the ORF that includes ACC
                     for minimal Kozak consensus"
     gene            23..>1009
                     /gene="CD86"
                     /db_xref="GeneID:942"
     CDS             23..>1009
                     /gene="CD86"
                     /note="missing stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="CD86 molecule"
                     /protein_id="ABM84713.1"
                     /db_xref="GeneID:942"
                     /translation="MDPQCTMGLSNILFVMAFLLSGAAPLKIQAYFNETADLPCQFAN
                     SQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQI
                     KDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSI
                     HGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFC
                     ILETDKTRLLSSPFSIELEDPQPPPDHIPWITAVLPTVIICVMVFCLILWKWKKKKRP
                     RNSYKCGTNTMEREESEQTKKREKIHIPERSDETQRVFKSSKTSSCDKSDTCF"
     misc_feature    1010..1013
                     /note="linker at the 3' end of the ORF"
     misc_feature    1014..1030
                     /note="attL2 site"
BASE COUNT          319 a          223 c          205 g          283 t
ORIGIN      
        1 gtacaaaaaa gcaggctcca ccatggatcc ccagtgcact atgggactga gtaacattct
       61 ctttgtgatg gccttcctgc tctctggtgc tgctcctctg aagattcaag cttatttcaa
      121 tgagactgca gacctgccat gccaatttgc aaactctcaa aaccaaagcc tgagtgagct
      181 agtagtattt tggcaggacc aggaaaactt ggttctgaat gaggtatact taggcaaaga
      241 gaaatttgac agtgttcatt ccaagtatat gggccgcaca agttttgatt cggacagttg
      301 gaccctgaga cttcacaatc ttcagatcaa ggacaagggc ttgtatcaat gtatcatcca
      361 tcacaaaaag cccacaggaa tgattcgcat ccaccagatg aattctgaac tgtcagtgct
      421 tgctaacttc agtcaacctg aaatagtacc aatttctaat ataacagaaa atgtgtacat
      481 aaatttgacc tgctcatcta tacacggtta cccagaacct aagaagatga gtgttttgct
      541 aagaaccaag aattcaacta tcgagtatga tggtattatg cagaaatctc aagataatgt
      601 cacagaactg tacgacgttt ccatcagctt gtctgtttca ttccctgatg ttacgagcaa
      661 tatgaccatc ttctgtattc tggaaactga caagacgcgg cttttatctt cacctttctc
      721 tatagagctt gaggaccctc agcctccccc agaccacatt ccttggatta cagctgtact
      781 tccaacagtt attatatgtg tgatggtttt ctgtctaatt ctatggaaat ggaagaagaa
      841 gaagcggcct cgcaactctt ataaatgtgg aaccaacaca atggagaggg aagagagtga
      901 acagaccaag aaaagagaaa aaatccatat acctgaaaga tctgatgaaa cccagcgtgt
      961 ttttaaaagt tcgaagacat cttcatgcga caaaagtgat acatgttttt tggacccagc
     1021 tttcttgtac
//