LOCUS DQ893787 1030 bp DNA linear SYN 14-JUL-2016 DEFINITION Synthetic construct Homo sapiens clone IMAGE:100008247; FLH164390.01L; RZPDo839F10161D CD86 molecule (CD86) gene, encodes complete protein. ACCESSION DQ893787 VERSION DQ893787.2 KEYWORDS Human ORF Project; ORFeome collaboration; Gateway cloning system; full-length ORF without stop codon; FLEXGene. SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. REFERENCE 1 (bases 1 to 1030) AUTHORS Rolfs,A., Kelley,F., McCarron,S., Jepson,D., Shen,B., Shi,Z., Hu,Y., Taycher,E., Zuo,D., Ebert,L., Hoerlein,A., Ernst,U., Korn,B. and LaBaer,J. TITLE Cloning of human full-length CDS FLEXGene in Gateway(TM) recombinational vector system JOURNAL Unpublished REFERENCE 2 (bases 1 to 1030) AUTHORS Rolfs,A., Kelley,F., McCarron,S., Jepson,D., Shen,B., Shi,Z., Hu,Y., Taycher,E., Zuo,D., Ebert,L., Hoerlein,A., Ernst,U., Korn,B. and LaBaer,J. TITLE Direct Submission JOURNAL Submitted (11-AUG-2006) Biological Chemistry and Molecular Pharmacology, Harvard Institute of Proteomics, 320 Charles Street, Cambridge, MA 02141, USA REFERENCE 3 (bases 1 to 1030) AUTHORS Rolfs,A., Kelley,F., McCarron,S., Jepson,D., Shen,B., Shi,Z., Hu,Y., Taycher,E., Zuo,D., Ebert,L., Hoerlein,A., Ernst,U., Korn,B. and LaBaer,J. TITLE Direct Submission JOURNAL Submitted (22-JAN-2007) Biological Chemistry and Molecular Pharmacology, Harvard Institute of Proteomics, 320 Charles Street, Cambridge, MA 02141, USA REMARK Sequence update by submitter COMMENT On Jan 22, 2007 this sequence version replaced DQ893787.1. This CDS clone is a part of a collection of human full-length ORF clones generated and verified by Harvard Institute of Proteomics (HIP) and Deutsches Ressourcenzentrum fuer Genomforschung GmbH (RZPD, Heubnerweg 6, D-14059 Berlin, Germany). This ORF clone has been cloned without stop-codon (to allow fusion with C-terminal tag). AttB recombination sites have been added to either end of the ORF and directionally cloned using the Gateway cloning system into pDONR221. Additional sequences in the clone: 'ACC' before the 'ATG' (corresponding to Kozak consensus sequences). Each clone is clonally isolated and full-length sequence-verified. This clone is available for distribution at HIP (http://plasmid.hms.harvard.edu/) and RZPD (http://www.rzpd.de/products/orfclones/rzpdexpression/). This clone is part of the international ORFeome Collaboration (http://www.orfeomecollaboration.org/). FEATURES Location/Qualifiers source 1..1030 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="IMAGE:100008247; FLH164390.01L; RZPDo839F10161D" /clone_lib="HIP_Gateway221_fusion" /focus /note="Vector: pDONR221; derived from parent clone IMAGE:5173789 (accession BC040261.1)" source 23..1009 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" misc_feature 1..17 /note="attL1 site" misc_feature 18..22 /note="linker at the 5' end of the ORF that includes ACC for minimal Kozak consensus" gene 23..>1009 /gene="CD86" /db_xref="GeneID:942" CDS 23..>1009 /gene="CD86" /note="missing stop codon" /codon_start=1 /transl_table=11 /product="CD86 molecule" /protein_id="ABM84713.1" /db_xref="GeneID:942" /translation="MDPQCTMGLSNILFVMAFLLSGAAPLKIQAYFNETADLPCQFAN SQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQI KDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSI HGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFC ILETDKTRLLSSPFSIELEDPQPPPDHIPWITAVLPTVIICVMVFCLILWKWKKKKRP RNSYKCGTNTMEREESEQTKKREKIHIPERSDETQRVFKSSKTSSCDKSDTCF" misc_feature 1010..1013 /note="linker at the 3' end of the ORF" misc_feature 1014..1030 /note="attL2 site" BASE COUNT 319 a 223 c 205 g 283 t ORIGIN 1 gtacaaaaaa gcaggctcca ccatggatcc ccagtgcact atgggactga gtaacattct 61 ctttgtgatg gccttcctgc tctctggtgc tgctcctctg aagattcaag cttatttcaa 121 tgagactgca gacctgccat gccaatttgc aaactctcaa aaccaaagcc tgagtgagct 181 agtagtattt tggcaggacc aggaaaactt ggttctgaat gaggtatact taggcaaaga 241 gaaatttgac agtgttcatt ccaagtatat gggccgcaca agttttgatt cggacagttg 301 gaccctgaga cttcacaatc ttcagatcaa ggacaagggc ttgtatcaat gtatcatcca 361 tcacaaaaag cccacaggaa tgattcgcat ccaccagatg aattctgaac tgtcagtgct 421 tgctaacttc agtcaacctg aaatagtacc aatttctaat ataacagaaa atgtgtacat 481 aaatttgacc tgctcatcta tacacggtta cccagaacct aagaagatga gtgttttgct 541 aagaaccaag aattcaacta tcgagtatga tggtattatg cagaaatctc aagataatgt 601 cacagaactg tacgacgttt ccatcagctt gtctgtttca ttccctgatg ttacgagcaa 661 tatgaccatc ttctgtattc tggaaactga caagacgcgg cttttatctt cacctttctc 721 tatagagctt gaggaccctc agcctccccc agaccacatt ccttggatta cagctgtact 781 tccaacagtt attatatgtg tgatggtttt ctgtctaatt ctatggaaat ggaagaagaa 841 gaagcggcct cgcaactctt ataaatgtgg aaccaacaca atggagaggg aagagagtga 901 acagaccaag aaaagagaaa aaatccatat acctgaaaga tctgatgaaa cccagcgtgt 961 ttttaaaagt tcgaagacat cttcatgcga caaaagtgat acatgttttt tggacccagc 1021 tttcttgtac //