LOCUS DQ119298 6372 bp DNA linear BCT 30-JUL-2005
DEFINITION Pseudomonas fluorescens feruloyl-CoA hydratase (ech), vanillin
hydratase (vdh), and feruloyl-CoA synthetase (fcs) genes, complete
cds.
ACCESSION DQ119298
VERSION DQ119298.1
KEYWORDS .
SOURCE Pseudomonas fluorescens
ORGANISM Pseudomonas fluorescens
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 6372)
AUTHORS Zhang,G. and Zhao,J.
TITLE Ferulate catabolism genes from Pseudomonas fluorescens JCM5963
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6372)
AUTHORS Zhang,G. and Zhao,J.
TITLE Direct Submission
JOURNAL Submitted (08-JUL-2005) Shenzhen Graduate School, Tsinghua
University, L401, Tsinghua Campus, University Town, Shenzhen
518055, P.R.China
FEATURES Location/Qualifiers
source 1..6372
/organism="Pseudomonas fluorescens"
/mol_type="genomic DNA"
/strain="JCM5963"
/type_material="type strain of Pseudomonas fluorescens"
/db_xref="taxon:294"
misc_feature 1..6372
/note="ferulate catabolism genes"
gene 1466..2296
/gene="ech"
CDS 1466..2296
/gene="ech"
/codon_start=1
/transl_table=11
/product="feruloyl-CoA hydratase"
/protein_id="AAZ23790.1"
/translation="MSKYEGRWTTVKVEIEEGIAWVILNRPEKRNAMSPTLNREMIDV
LETLEQDPAAGVLVLTGAGEAWTAGMDLKEYFREVDAGPEILQEKIRREASQWQWKLL
RMYAKPTIAMVNGWCFGGGFSPLVACDLAICADEATFGLSEINWGIPPGNLVSKAMAD
TVGHRQSLYYIMTGKTFGGQKAAEMGLVNESVPLAQLREVTIELARNLLEKNPVVLRA
AKHGFKRCRELTWEQNEDYLYAKLDQSRLLDTEGGREQGMKQFLDDKSIKPGLQAYKR
"
gene 2373..3821
/gene="vdh"
CDS 2373..3821
/gene="vdh"
/codon_start=1
/transl_table=11
/product="vanillin hydratase"
/protein_id="AAZ23791.1"
/translation="MLDVPLLIGGQSCPARDGRTFERRNPVTGEVVSRVAAATLEDAD
AAVAAAQAAFPAWVALAPNERRTRLLKAAEQLQARSSEFIAAAGETGAMANWYGFNVR
LAANMLREAASMTTQITGEVIPSDVPGSFAMALRQPCGVVLGIAPWNAPVILATRAIA
MPLACGNTVVLKASEISPAVHRLIGQVLQDAGLGDGVVNVISNAPADAAAIVERLIAN
PAVRRVNFTGSTHVGRIVGELSARHLKPALLELGGKAPLLVLDDADLDAAVAAAAFGA
YFNQGQICMSTERLIVDAKVADAFTAKLAAKVATLHAGDPAAADSVLGSLVDASAGTR
IKALIDDALAKGARLVAGGQLAGSILQPTLIEGVTEQMRVYREESFGPVAVLLRGEGD
EALLRLANDSEFGLSAAIFSRDTGRALALAQRVESGICHINGPTVHDEAQMPFGGVKS
SGYGSFGGKASIEHFTQLRWVTLQNGPRHYPI"
gene 3989..5758
/gene="fcs"
CDS 3989..5758
/gene="fcs"
/codon_start=1
/transl_table=11
/product="feruloyl-CoA synthetase"
/protein_id="AAZ23792.1"
/translation="MRSLEPLAELPARLLDRLVHWARVRPEQTFIAARQAGGDWRRVS
YREMLDGVRAIAQGLLSYGLSADKPLALLSGNDIEHLQVALGAMYAGIPYCPVSPAYS
LLSQDFAKLRHVCDLLQPGLVFVSDAPAYQRAIDAVLPPETPLISVRGQVPGRTQISF
ASLLHTSAGTEADAAFNATGPDSIAKFLFTSGSTKLPKAVITTQRMLCANQQMLLQTF
PVFGEEPPVLVDWLPWNHTFGGSHNVGIVLYNGGTFYLDDGKPTAQGFAQTLRNLKEI
SPTAYLTVPKGWEELVNALEQDAALRERFFARMKLFFFAAAGLSQSVWDRLDRVAEQH
CGERIRMMAGLGMTEAAPSCTFTTGPLSMAGYIGLPAPGCEVRLVPVDGKFEGRFRGP
HIMPGYWRAPEQTAEVFDEQGYYCSGDAIKLADPHQPHWGLMFDGRIAEDFKLSSGVF
VSVGPLRNRAVLEGSPYVQDLVIAAPDRECLGALVFPRAYECRRLSGLNAEASDAEVL
NSAPVRQWFGDWLQRLNREATGNASRLEWIAVLDEPASIDRGEITDKGSINQRAVLQW
RAAKVEALYRGEDPTILRAGPKP"
BASE COUNT 1192 a 1991 c 2047 g 1142 t
ORIGIN
1 gaattcacaa ctggcagcga tagcgccgac gctgctgccg gcgtcgagag tcaggacgat
61 gctcaatgaa cgcctctgta gaccttcagc gcaccgagca gtggcaccag gaccgcagcg
121 ggcgactcac cgccagccga ttcaaggatg ttattgcatg ggccgaccgt gacaaacacg
181 gcaaacgcaa gccgcttgcg gcccgtacca cttacatgcg cgagctggct tttgagcgcc
241 tggccaaccg atcgaaacat tcggtaagca gcaagtcgat ggcctgggga accgaggttg
301 agcagtcgag ccacgacttc tacgaaatcc tgactggtaa tagtgtcatc aagtcgggct
361 tcgtagttca tccaaaatac gactggctgg gctgttcgcc ggacggcttg attggcgagg
421 acgggggtat tgagtcgaaa tgcccattca atgaggccgt tcacgtccgc acctggctcg
481 aaggcatgcc cgatgaacat aagccgcagg ttcagggctg catgttcgtc acgggccggg
541 ggtggtggga tttcctatca ttcgatccac gtcaggatga agactgccga ctgtatatcg
601 aaaccattga gcgcgatgag gagtacatcg cgatgcttca tcaagagctg gtccagttca
661 atctggaact tggcaggatg gttgatgaag tcgcggacaa agcccgggcg caagcccatc
721 gcctaggagc ctgatcatga tcagcaacca cctcagcatg atcgaggaac tgcgaccggt
781 ctcggatgag ctggccgccc agatcgccga gttcgtagca gctggcggcg aaatcgaagt
841 ggccgaacct ccaccgccac cgaagccagt ggtgtatgtg ccccaggagc ccccggcgcc
901 gaagccattt gttcgtcgtc gggttgaagc acctcccctg cccctcgaca agaatgacat
961 ccgcacccgg gcacgcctca agcttgttga gcagatacgc cagctcggcg tcacgcacac
1021 ccagaccgaa gtcgccgctg cacttggtgt aagccgacgg ctaatctata accacgctgc
1081 ccgctatgac atcaccttcc agcctccaac ccggggcggc gcacgcaacc tggtgcacaa
1141 caccgtcgac aaggagcgcg acgcgaagta tgtcgagcgg atcagggcat tcatggagct
1201 gggcatcagc cggcgccagt gctgcgggaa gctcgccatc agcaataagg ccttcgagcg
1261 gatcatcgcg gcccacggca tcgactatcc caaagcgcgc caaggcagaa cctcatgcgc
1321 cgcatagccc gcatccagca acgcaaacgc caaacctggc tcgcactgcc ggccagagga
1381 atagcggatt aatagttaat tgacataact atatttgcgg tttaatttcg aatcatcttc
1441 acacccagaa caagagcgta ccgccatgag caaatacgaa ggtcgctgga ccacagtcaa
1501 ggttgagatc gaagaaggca tcgcctgggt cattctcaat cgtccggaaa aacgcaacgc
1561 catgagcccg accctgaacc gggaaatgat cgacgtcctg gaaaccctcg agcaggatcc
1621 tgccgccggc gtgctggtcc tcaccggtgc gggcgaagcc tggacggcgg gcatggacct
1681 caaggaatac tttcgcgaag tggatgccgg cccggaaatc ctccaggaga aaatccgccg
1741 cgaagcctcg caatggcaat ggaaactgct gcgcatgtac gccaagccga ccatcgccat
1801 ggtcaatggc tggtgcttcg gcggtggctt cagcccgctg gtggcgtgcg acctggcgat
1861 ctgcgccgac gaagcgacct tcggcctttc ggaaatcaac tggggcatcc cgccgggtaa
1921 cctggtgagc aaggccatgg ccgacaccgt gggccatcgt caatcgctgt actacatcat
1981 gaccggcaag acctttggcg ggcagaaagc cgccgaaatg ggccttgtca acgaaagcgt
2041 gccgctggcg caactgcgcg aagtgaccat cgaactggcg cgtaacctgc tcgagaaaaa
2101 cccggtggtg ctgcgtgccg ccaagcatgg cttcaagcgc tgccgcgaac tgacctggga
2161 gcagaacgag gattacctct acgccaagct cgatcagtcg cgtctgctcg acaccgaagg
2221 cggtcgcgag cagggcatga aacagttcct cgacgacaag agcatcaagc ctggcctgca
2281 ggcctataaa cgctgaaggg cgcggccgcc tggccgcgcc gttgtaccct gcttgaacgt
2341 gttcccgata aagacaataa agaggaatca ccatgctgga cgtgcccctg ttgatcggcg
2401 gccagtcgtg ccccgcccgt gacggtcgaa ccttcgaacg tcgcaacccg gtgaccggtg
2461 aagtggtctc gcgggtggcc gctgcgaccc tggaagatgc cgacgccgcc gtcgccgccg
2521 cccaagccgc gttccctgcc tgggtggcgc tggcgcccaa cgaacgccgc acccgcttgc
2581 tcaaggctgc cgagcaattg caggcgcgca gcagcgaatt catcgccgcc gccggcgaga
2641 ccggcgccat ggccaactgg tatggcttca atgtgcgcct ggcggccaat atgctgcgcg
2701 aagcggcgtc gatgaccacc cagatcacag gcgaggtgat tccctcggat gtccccggca
2761 gtttcgccat ggccttgcgt caaccgtgcg gcgtggtgct gggcatcgcg ccgtggaacg
2821 cgccggtcat cctcgccact cgcgccatcg ccatgccgct ggcctgcggg aataccgtgg
2881 tgcttaaggc ctcggaaata agcccggcgg tgcaccgcct gattggccag gtattgcagg
2941 atgcaggctt gggcgatggc gtggtcaacg tcatcagcaa tgccccggcg gatgccgccg
3001 cgattgtcga gcggctgatc gccaacccgg cggtgcggcg ggtcaacttc accggttcga
3061 cccatgtcgg gcggattgtc ggcgagctct cggcgcgtca cctcaagccg gcgttgctgg
3121 aactgggtgg caaggcgccg ttgctggtgc tcgacgatgc cgatcttgac gctgctgtag
3181 cggcggcggc gtttggcgcc tatttcaacc agggccagat ttgcatgtcc accgaacgcc
3241 tgatcgtcga tgccaaggtc gccgatgcgt tcaccgccaa gctggcggcc aaggtcgcga
3301 ccctgcatgc gggtgatccc gcggcggcag attcggtatt ggggtcgctg gtggacgcca
3361 gcgccggcac acgcatcaag gccctgatcg acgatgccct ggccaagggc gcgcggctgg
3421 tggcaggcgg ccaactggcg ggcagcatcc tgcagccgac cctgatcgag ggtgtcaccg
3481 aacagatgcg cgtgtaccgc gaagagtcct ttggcccggt cgccgtactg ctgcgtggtg
3541 agggtgatga agccttgttg cggctggcca acgattccga gttcggcctg tcggcggcga
3601 tcttcagtcg agacaccggg cgcgcgctgg cgctggcgca gcgggtcgag tcgggtattt
3661 gccatatcaa tggcccgacg gtgcatgacg aggcgcagat gccgtttggt ggggtcaagt
3721 ccagtgggta cggcagcttt ggtggcaagg cgtccattga gcacttcacg caattgcgct
3781 gggtgacctt gcagaacggg ccacggcatt acccgatctg agcagttgct gcgacctaac
3841 aataacaagg cggctgtgat ccgccttgct ggaggacacc cttgtgagtt ccgaattccg
3901 accgcaaccc cagcccgcac cgcgataccg cgaggtctcc atcggccacg caccggtggc
3961 ggtcactgaa gaacagggcg tgttgcacat gcgctccctg gaacccctgg ccgagttgcc
4021 ggcgcgcttg ctcgatcgcc tggtgcattg ggcgcgggtg cggcccgagc agacgtttat
4081 cgcggcacgc caggccggcg gtgattggcg ccgtgtcagc taccgcgaga tgctcgacgg
4141 cgtacgcgcg attgcccagg gcctgctgag ttacgggctg tcggcggaca agccgctggc
4201 attgctgtcg ggcaacgaca tcgaacattt gcaggtcgcc ctcggcgcga tgtacgccgg
4261 tattccttac tgcccggtat cgccggcgta ttcgttgttg tcccaggatt tcgccaagct
4321 gcggcatgtc tgtgacctgc tgcaacccgg gctggtgttc gtcagcgatg cgccggccta
4381 ccagcgggcg atcgatgccg tattgccgcc cgaaacgccg ctgatcagcg tgcgcggcca
4441 ggtgcccggg cgcactcaga tcagcttcgc cagcctgttg cacacgtcgg ccggtactga
4501 ggccgacgcg gctttcaacg ccaccggccc cgacagcatt gccaaattcc tgtttacctc
4561 gggctcgacc aaactgccca aggcagtgat caccactcag cgcatgctgt gcgccaacca
4621 gcaaatgctc ttgcagacat ttccggtgtt cggcgaggag ccgccggtgc tggtggactg
4681 gctgccgtgg aatcacacct ttggcggcag ccacaacgtc ggcatcgtgc tctacaacgg
4741 cggcaccttt tacctcgacg acggcaaacc cacagcccag ggttttgccc agaccctgcg
4801 taacctcaag gaaatttcgc ccaccgccta cctgacagtg cccaagggct gggaagaatt
4861 ggtcaacgcc ctggaacagg acgccgcgtt gcgcgagcgg ttctttgcgc ggatgaaact
4921 gttcttcttc gctgctgccg gcctgtcgca aagtgtctgg gaccgcctcg accgcgtggc
4981 cgaacagcac tgcggcgagc gtatccgcat gatggccggg ctgggcatga ccgaagccgc
5041 gccgtcctgc accttcacca ccgggccgtt gtccatggcc ggttatatcg gcttgcccgc
5101 gccgggctgc gaggtgcgcc tggtgccggt ggacggcaag ttcgaagggc gttttcgcgg
5161 cccgcacatc atgccggggt attggcgcgc gcccgagcaa accgccgagg tgttcgatga
5221 gcaggggtat tactgttccg gtgatgcgat caagctcgcc gatccccatc agccacactg
5281 ggggctgatg ttcgacgggc gtattgccga agacttcaag ctgtcctccg gggtgtttgt
5341 cagcgtcggt ccgttgcgca atcgtgcggt gctcgaaggc tcaccctatg tgcaggacct
5401 ggtgatcgcc gcgccggacc gcgaatgcct gggagcgctg gtgttcccca gggcctatga
5461 atgccgccgg ctgtcgggct tgaacgccga ggccagcgac gccgaggtgc tcaacagcgc
5521 gccggtgcgc caatggttcg gcgactggct gcaacggctc aatcgcgagg ccaccggcaa
5581 tgccagtcgc ctggagtgga ttgccgtgct cgacgagccg gcgtccatcg accgtgggga
5641 aatcaccgac aagggctcga tcaaccagcg cgcggtattg caatggcgtg cggccaaggt
5701 tgaagcgctg tatcgcggcg aagacccgac aatcctgcgt gccgggccca aaccgtgagc
5761 ggcgccgggc agtattcggc gtttgccgat gcggcgatgt tcgaggccgt gcgcacgccg
5821 tgggttgacc tcggcggggc gctgtcccag gtgtcgccca tcgacctggg catcaaggtt
5881 ggccgtgaag tgctggctcg cgccggcctc caggggcagg cggttgacag tgtgctggcc
5941 ggttccatgg cccaggccag cttcgatgcc tatttgctgt cacggcatat cggcttgtac
6001 agcggtgtgg cgcaagcggt cccggcgctg ggggtgcagc gcatctgcgc taccggcttc
6061 gagctgttgc gccaggccgc tgaacaactg cgcgacgagg tgcaactggc cttgtgcgtg
6121 gcggctgaat cgatgtcacg caacccgatt gcggcctaca cccaccgcgg tgggttccgc
6181 ctggggggcg aagtgcaatt caaggatttc ctctgggaag cgctgtacga cccggcccca
6241 ggtctggaca tgatcgccac tgccgataac ctggcgcggc gttatggcct gagtcgcgag
6301 gccgtggacc gttatgcctg cgccagccac cagcgcgcgt tgcaggcgca acaggagggt
6361 gcctgggtcg ac
//