LOCUS CR457012 732 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G095D for gene CD48, CD48 antigen (B-cell membrane protein); complete cds, incl. stopcodon. ACCESSION CR457012 VERSION CR457012.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 732) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 732) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834G095D, ORFNo 1554 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G095D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_001778 we found amino acid exchange(s) at position (first base of changed triplet): 514(phe->leu) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..732 /db_xref="H-InvDB:HIT000267862" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834G095D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..732 /codon_start=1 /gene="CD48" /db_xref="GOA:Q6IAZ2" /db_xref="H-InvDB:HIT000267862.12" /db_xref="InterPro:IPR003599" /db_xref="InterPro:IPR007110" /db_xref="InterPro:IPR013106" /db_xref="InterPro:IPR013783" /db_xref="InterPro:IPR033548" /db_xref="InterPro:IPR036179" /db_xref="UniProtKB/TrEMBL:Q6IAZ2" /protein_id="CAG33293.1" /translation="MCSRGWDSCLALELLLLPLSLLVTSIQGHLVHMTVVSGSNVTLN ISESLPENYKQLTWFYTFDQKIVEWDSRKSKYFESKFKGRVRLDPQSGALYISKVQKE DNSTYIMRVLKKTGNEQEWKIKLQVLDPVPKPVIKIEKIEDMDDNCYLKLSCVIPGES VNYTWYGDKRPLPKELQNSVLETTLMPHNYSRCYTCQVSNSVSSKNGTVCLSPPCTLA RSFGVEWIASWLVVTVPTILGLLLT" BASE COUNT 202 a 173 c 176 g 181 t ORIGIN 1 atgtgctcca gaggttggga ttcgtgtctg gctctggaat tgctactgct gcctctgtca 61 ctcctggtga ccagcattca aggtcacttg gtacatatga ccgtggtctc cggcagcaac 121 gtgactctga acatctctga gagcctgcct gagaactaca aacaactaac ctggttttat 181 actttcgacc agaagattgt agaatgggat tccagaaaat ctaagtactt tgaatccaaa 241 tttaaaggca gggtcagact tgatcctcag agtggcgcac tgtacatctc taaggtccag 301 aaagaggaca acagcaccta catcatgagg gtgttgaaaa agactgggaa tgagcaagaa 361 tggaagatca agctgcaagt gcttgaccct gtacccaagc ctgtcatcaa aattgagaag 421 atagaagaca tggatgacaa ctgttatctg aaactgtcat gtgtgatacc tggcgagtct 481 gtaaactaca cctggtatgg ggacaaaagg cccctcccaa aggagctcca gaacagtgtg 541 cttgaaacca cccttatgcc acataattac tccaggtgtt atacttgcca agtcagcaat 601 tctgtgagca gcaagaatgg cacggtctgc ctcagtccac cctgtaccct ggcccggtcc 661 tttggagtag aatggattgc aagttggcta gtggtcacgg tgcccaccat tcttggcctg 721 ttacttactt aa //