LOCUS BT025950 4312 bp mRNA linear INV 14-JUL-2016
DEFINITION Drosophila melanogaster IP15231 full insert cDNA.
ACCESSION BT025950
VERSION BT025950.1
KEYWORDS FLI_CDNA.
SOURCE Drosophila melanogaster (fruit fly)
ORGANISM Drosophila melanogaster
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
Pterygota; Neoptera; Endopterygota; Diptera; Brachycera;
Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
REFERENCE 1 (bases 1 to 4312)
AUTHORS Stapleton,M., Carlson,J., Chavez,C., Frise,E., George,R.,
Pacleb,J., Park,S., Wan,K., Yu,C. and Celniker,S.
TITLE Direct Submission
JOURNAL Submitted (08-JUN-2006) Berkeley Drosophila Genome Project,
Lawrence Berkeley National Laboratory, One Cyclotron Road,
Berkeley, CA 94720, USA
COMMENT Sequence submitted by:
Berkeley Drosophila Genome Project
Lawrence Berkeley National Laboratory
Berkeley, CA 94720
This clone was sequenced as part of a high-throughput process to
sequence clones from the Drosophila Gene Collection The sequence
has been subjected to integrity checks for sequence accuracy,
presence of a polyA tail and contiguity within 100 kb in the
genome. Thus we believe the sequence to reflect accurately this
particular cDNA clone. However, there are artifacts associated with
the generation of cDNA clones that may have not been detected in
our initial analyses such as internal priming, priming from
contaminating genomic DNA, retained introns due to reverse
transcription of unspliced precursor RNAs, and reverse
transcriptase errors that result in single base changes. For
further information about this sequence, including its location and
relationship to other sequences, please visit our Web site
(http://www.fruitfly.org) or send email to cdna@fruitfly.org.
FEATURES Location/Qualifiers
source 1..4312
/organism="Drosophila melanogaster"
/mol_type="mRNA"
/db_xref="taxon:7227"
gene 1..>4312
/gene="Dscam"
/note="blast alignment with CG17800-RC"
/map="43B1-43B1"
/db_xref="FLYBASE:FBgn0033159"
misc_feature 1..4311
/note="sim4 alignment with 2R_rel3:2391350-2442770"
misc_feature 1..4311
/note="this clone was identified by inverse PCR using
templates from pooled GH, LD, LP, and SD cDNA libraries"
CDS 520..>4312
/gene="Dscam"
/note="Longest ORF"
/codon_start=1
/product="IP15231p"
/protein_id="ABG02194.1"
/db_xref="FLYBASE:FBgn0033159"
/translation="MNMPNERLKWLMLFAAVALIACGSQTLAANPPDADQKGPVFLKE
PTNRIDFSNSTGAEIECKASGNPMPEIIWIRSDGTAVGDVPGLRQISSDGKLVFPPFR
AEDYRQEVHAQVYACLARNQFGSIISRDVHVRAVVSQHYEEDIHKAFVIRGNSAILKC
DIPSFVADFVNVISWHSDEKENFYPGTEYDGKYLVLPSGELHIREVGPEDGYKSYQCR
TKHRLTGETRLSATKGRLVITEPVGSVGPKLTSGDDSRTVRIRQEDSVTLLCPAQAYP
VPVYRWYKFIEGTTRKQAVVLNDRVKQVSGTLIIKDAVVEDSGKYLCVVNNSVGGESV
ETVLTVTAPLSAKIDPPTQTVDFGRPAVFTCQYTGNPIKTVSWMKDGKAIGHSEPVLR
IESVKKEDKGMYQCFVRNDQESAEASAELKLGGRFDPPVIRQAFQEETMEPGPSVFLK
CVAGGNPTPEISWELDGKKIANNDRYQVGQYVTVNGDVVSYLNITSVHANDGGLYKCI
AKSKVGVAEHSAKLNVYGLPYIRQMEKKAIVAGETLIVTCPVAGYPIDSIVWERDNRA
LPINRKQKVFPNGTLIIENVERNSDQATYTCVAKNQEGYSARGSLEVQVMVLPRIIPF
AFEEGPAQVGQYLTLHCSVPGGDLPLNIDWTLDGQAISEDLGITTSRVGRRGSVLTIE
AVEASHAGNFTCHARNLAGHQQFTTPLNVYVPPRWILEPTDKAFAQGSDAKVECKADG
FPKPQVTWKKAVGDTPGEYKDLKKSDNIRVEEGTLHVDNIQKTNEGYYLCEAINGIGS
GLSAVIMISVQAPPEFTEKLRNQTARRGEPAVLQCEAKGEKPIGILWNMNNMRLDPKN
DNRYTIREEILSTGVMSSLSIKRTERSDSALFTCVATNAFGSDDASINMIVQEVPEMP
YALKVLDKSGRSVQLSWAQPYDGNSPLDRYIIEFKRSRASWSEIDRVIVPGHTTEAQV
QKLSPATTYNIRIVAENAIGTSQSSEAVTIITAEEAPSGKPQNIKVEPVNQTTMRVTW
KPPPRTEWNGEILGYYVGYKLSNTNSSYVFETINFITEEGKEHNLELQNLRVYTQYSV
VIQAFNKIGAGPLSEEEKQFTAEGTPSQPPSDTACTTLTSQTIRVGWVSPPLESANGV
IKTYKVVYAPSDEWYDETKRHYKKTASSDTVLHGLKKYTNYTMQVLATTAGGDGVRSV
PIHCQTEPDVPEAPTDVKALVMGNAAILVSWRPPAQPNGIITQYTVYSKAEGAETETK
TQ"
BASE COUNT 1114 a 1238 c 1123 g 837 t
ORIGIN
1 agaaccggat ttcagcgcta gtcggcgaat cgcgagagcg tgcgagtatt tgtgtgtgcg
61 tgtgagcgtt tgttttggga caccgatacg gatacgggta cggatagacg gatatacggc
121 agcggtacgc gattagcaat ccgccaaccg gttgcagcgg tgctagtgca ccaccctaca
181 aaaaagtgaa taatagtatg caaagtatct gaaagatagt caagagcaga gcaaagtatc
241 tgtgtttgtg tgtgccgtgc ttttgtgcgg agtcgccacc cccgtggatt agtggcaccc
301 tgctgcaaat agcccaccac tctcacaagt gtgtgtgtgt gagtgttaac aattaatcgc
361 atttaaaaaa caatttggcc agccgcagta acaataacga taattcggac gtgccaaggg
421 tgcttttcag tggatttttg cgtaaaatcg atagtcagat agacccgctc tctactgcca
481 acttcacaca acccgctcag cggaaacacg gcatacgaaa tgaatatgcc caacgaacgc
541 ctcaaatggc taatgctgtt cgcagctgtt gccctcattg cttgtggtag tcagacccta
601 gctgccaatc ccccagatgc cgaccaaaaa ggacccgtct tcctcaagga acccaccaac
661 cgcattgact tctccaactc cacgggcgca gagatcgagt gcaaggccag cggcaatccc
721 atgcccgaga ttatttggat caggagcgac ggtaccgccg tgggtgatgt gcccggattg
781 cgtcagatct catccgacgg caagctggtc ttccctccat tccgcgccga ggactaccgc
841 caggaggtcc atgcccaggt gtacgcctgc ctggcccgca accagttcgg atccattatc
901 tcccgggacg tccatgtgcg agccgtcgtt tcccaacact acgaagaaga tatacacaag
961 gcatttgtca tccgcggcaa ttccgccata ttgaaatgcg atataccgtc gtttgtagcc
1021 gatttcgtga atgttatatc ctggcatagt gacgagaaag agaatttcta tccaggcact
1081 gaatatgatg gaaagtacct ggtattgccc tctggagagc tgcacatccg tgaggttgga
1141 cccgaggacg gatacaagag ctaccagtgc cgaaccaaac atcgtctgac cggagaaacc
1201 cgattaagtg ccacaaaagg acgattggtc atcacagaac ccgttggcag cgttgggcca
1261 aaactcacat caggcgacga ttcgcggacg gtgaggatta ggcaggagga tagtgttacg
1321 ctcctttgtc cagctcaagc ttatcccgtg ccggtgtata gatggtacaa gttcatcgaa
1381 ggaacaaccc gtaaacaagc tgtggtgcta aatgaccgtg tgaagcaggt ctctggtact
1441 ctcattatta aggacgcagt agtcgaggac tccggaaaat acctgtgcgt ggtgaacaat
1501 tccgtgggag gcgaaagcgt tgagactgtg cttaccgtca ctgcccccct atccgctaag
1561 atcgatcctc ccacacagac agttgacttc ggacgccccg ccgtattcac ttgccagtac
1621 actggaaatc ccatcaagac cgttagctgg atgaaggacg gcaaggctat cggacacagc
1681 gaacccgtgc tgcgcatcga atccgtgaag aaggaggaca agggcatgta ccagtgcttc
1741 gtaagaaacg accaggaatc ggcggaggcg agtgctgagc tgaagctcgg aggccgtttc
1801 gatccccccg tcatccgaca ggccttccag gaagagacca tggaacccgg accaagtgta
1861 ttcctcaagt gcgtcgccgg cggaaacccc acgcccgaaa tttcctggga actcgacggc
1921 aagaaaatcg ccaacaacga tcgctatcag gtcggacagt atgtgacggt caacggagat
1981 gtggtttcct atctgaacat aacctcggtc cacgccaacg atggcggtct ctacaagtgc
2041 atagccaaga gcaaagtggg cgtggccgaa cactcggcca agttgaatgt atatggactg
2101 ccctacatcc ggcaaatgga gaagaaggca attgttgctg gagaaaccct gattgtcaca
2161 tgtcctgtag ctggataccc catcgattcg attgtctggg agcgagataa ccgtgccctg
2221 cccatcaacc gcaaacagaa ggtcttcccc aacggaacgc tgattatcga gaatgtggaa
2281 cggaactcag accaagcgac ctacacttgc gttgccaaga atcaggaagg atactctgct
2341 cgaggatctc tggaagtgca agtcatggtt cttccgcgca tcataccctt cgccttcgag
2401 gagggtcctg cccaggtggg tcagtacctg acactgcact gctccgttcc cggcggagat
2461 ctgcccctga acatcgactg gacgctggat ggtcaggcaa tctcggagga tctgggaata
2521 accacgtccc gcgtgggtcg tcgtggaagt gtgctaacca tcgaggccgt ggaggcctca
2581 catgcgggca acttcacctg ccacgcccgc aatttggcgg gccatcagca gttcaccacg
2641 ccgctgaacg tctacgtacc tcccagatgg atcctcgagc ccaccgataa ggccttcgcc
2701 cagggatccg atgccaaggt tgaatgcaag gctgatggct tccccaaacc ccaagttaca
2761 tggaagaaag cagttggcga cacccccgga gagtacaagg atctaaagaa gagcgacaac
2821 atccgcgtgg aagagggcac tctgcatgtc gacaacatcc aaaagaccaa cgagggctac
2881 tatctgtgcg aggctatcaa tggaataggc tccggcctct cggctgtgat tatgatcagc
2941 gttcaggccc ctccagagtt cacggagaag ctgcgcaacc agaccgcccg acgaggagaa
3001 cccgccgtac tccagtgcga ggcgaagggc gagaagccca ttggcatctt gtggaacatg
3061 aacaacatgc gactggaccc aaagaacgac aatcggtaca ccattcgtga ggagatcctt
3121 tccaccggag ttatgtccag tctgtcgatc aagcgcaccg agagatccga ctccgcccta
3181 ttcacctgtg tcgccactaa tgcctttgga tccgatgacg ccagcataaa tatgattgtc
3241 caggaagttc cggagatgcc atatgctttg aaggtactcg acaaatccgg acgttccgtg
3301 cagctgagct gggcgcaacc ctacgatggc aactcccctc tggacaggta catcattgag
3361 tttaagcgtt ctcgtgcctc ctggagcgaa attgatcgcg tcatcgtgcc cggacacacc
3421 acagaagccc aagtgcagaa gctcagcccc gccaccacct acaacattcg catcgtggcc
3481 gaaaacgcca tcggcacatc ccaatcctct gaagcggtca ctatcatcac agccgaagaa
3541 gcaccctccg gcaagcccca gaacatcaag gtagaacccg tgaaccagac caccatgcgc
3601 gtcacctgga agccaccacc acgcaccgag tggaacggag agatcctggg ctactacgtc
3661 ggctacaagc tatccaacac taactcgtcc tacgttttcg agaccatcaa cttcatcacc
3721 gaggagggca aggagcacaa cctggaactg cagaacctgc gcgtgtacac ccagtactcc
3781 gtggtgatcc aggccttcaa caagatcgga gctggtccac tgagcgaaga ggagaaacaa
3841 ttcacggccg agggaacgcc cagccagcca cccagtgaca ccgcctgcac caccttaact
3901 tcgcagacca ttcgcgtcgg atgggtgagc ccacccctgg aatccgccaa cggagtgatc
3961 aagacctata aggttgtcta tgcccccagt gacgagtggt atgacgagac caagcgacac
4021 tataagaaga cggcctcctc cgacacagtc ctccatggct taaagaagta caccaactac
4081 acaatgcagg tcctggccac aacagccgga ggcgatggcg tccgcagcgt tcccatccac
4141 tgccaaaccg aacccgatgt tcccgaagca cccactgatg taaaggctct tgttatgggt
4201 aacgccgcta tcctggtatc ctggcgccca ccagcacagc caaacggcat tatcacccag
4261 tacaccgtgt actccaaggc cgaaggcgct gagactgaga caaagaccca ga
//