LOCUS BC156682 2254 bp DNA linear SYN 23-JAN-2008
DEFINITION Synthetic construct Mus musculus clone IMAGE:100062120, MGC:190368
guanylate cyclase 1, soluble, alpha 2 (Gucy1a2) mRNA, encodes
complete protein.
ACCESSION BC156682
VERSION BC156682.1
KEYWORDS MGC; ORFeome collaboration; Gateway cloning system; full-length ORF
with stop codon; de novo synthesis.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 2254)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2254)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-DEC-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT This clone is a part of a collection of full-length cDNA clones
generated by Mammalian Gene Collection project. DNA synthesis was
used to prepare the protein coding sequence (ORF) and flanking
sequences, permitting directional cloning into the Gateway Entry
vector, pENTR223.1 (Details are described in
http://mgc.nci.nih.gov/Vectors/prot_pENTR223.1).
Clone structure of the ORF and flanking sequences is as follows:
5'- gtac aaa aaa gca gaa gGG CCG TCA AGG CCC ACC 5'ORF3' TAA GGC
CTC ATG Ggc cca gct ttc ttg-3'.
This clone is also a part of ORFeome collaboration clones
(http://www.orfeomecollaboration.org/).
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Geneart
cDNA Library Preparation: GeneArt
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Geneart - the gene of your choice:
www.geneart.com
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCW Plate: 12 Row: e Column: 12.
FEATURES Location/Qualifiers
source 1..2254
/organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
/clone="MGC:190368 IMAGE:100062120"
/clone_lib="NIH_MGC_426"
/lab_host="DH10B"
/focus
/note="Vector: pENTR223.1 with stop codon"
source 35..2227
/organism="Mus musculus"
/mol_type="other DNA"
/db_xref="taxon:10090"
misc_feature 1..17
/note="attL1 site"
misc_feature 18..34
/note="linker at the 5' end of the ORF that includes Sfi I
site and ACC for minimal Kozak consensus"
gene 35..2227
/gene="Gucy1a2"
/gene_synonym="9430053E15"
/db_xref="GeneID:234889"
/db_xref="MGI:MGI:2660877"
CDS 35..2227
/gene="Gucy1a2"
/gene_synonym="9430053E15"
/codon_start=1
/product="guanylate cyclase 1, soluble, alpha 2"
/protein_id="AAI56683.1"
/db_xref="GeneID:234889"
/db_xref="MGI:MGI:2660877"
/translation="MSRRKISSESFSSLSSDYLETSPEEEGECPLSRLCWNGSRSPPG
PPGSREAAKAATSAPAASAAAASAAVAAEYKRAQRRGRVNLDSLGESISLLTAPLPQT
IHMTLKRTLQYYEHQVIGYRDAEKNFHNISKRCSYADHSNKEEIEDVSGILQCTANVL
GLQFQEIQERFGEEFFKICFDENERVLRAVGSTLQDFFNGFDALLEHIRTSFGKQATL
ESSSFLCKELPEGTLMLHYFHPHHTVGFAMLGMIKAAGKRIYHLNVEVEQIENEKLCF
DGSNPSNCSCLTFLIKECETTQITKDNPQGTSQVPADLRISINTFCRAFPFHLMFDSN
MVVLQLGEGLRKQLRYDTHRALKFEDCFEIVSPMINATFDRVLLRLSTPFVIRTKPEA
SGTENEDKVMEIKGQMIHVPESSAILFLGSPCVDKLDELMGRGLHLSDIPIHDATRDV
ILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKKKTVDLLYSIFPGDVAQQLWQGQ
QVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGLLDIYKVETI
GDAYCVASGLHRKSLCHAKPIALMALKMMELSEEVLTPDGKAIQMRIGIHSGSVLAGV
VGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLLKREDSFTFIPRSREELPD
NFPKEIPGVCYFLELRTDPKPPKPSLSSSRIKKVSYNIGTMFLRETSL"
misc_feature 2225..2237
/note="linker at the 3' end of the ORF that includes stop
codon TAA and Sfi I linker"
misc_feature 2238..2254
/note="attL2 site"
BASE COUNT 635 a 531 c 529 g 559 t
ORIGIN
1 gtacaaaaaa gcagaagggc cgtcaaggcc caccatgtct cgaagaaaga tttcatccga
61 gtctttcagc tctttgagct ctgactacct ggagaccagc ccggaggagg aaggggagtg
121 cccattgtcc aggctctgct ggaatggcag ccggagcccg ccagggccac ctggctctag
181 agaggccgct aaggctgcca cttcggcccc cgctgcctct gccgccgcgg cctctgctgc
241 agtcgccgcg gagtacaaga gagcccaacg ccgcggaagg gtcaacctgg actcactagg
301 cgaaagcatc agcctcctga cggcgccctt gcctcagacg atacacatga ctctcaagag
361 gacattgcag tattatgaac accaagttat tggttatagg gatgcagaaa agaatttcca
421 caatatctct aaaagatgct cctatgcaga ccattccaac aaagaggaaa ttgaagatgt
481 ctcaggaatt cttcagtgca ctgctaatgt actcggactg cagttccaag aaattcaaga
541 aagatttggt gaggaattct ttaagatatg ctttgatgag aatgaaagag tccttcgagc
601 tgtgggcagc acattgcagg acttcttcaa tggctttgat gcattgttag aacacattag
661 gacttctttt gggaaacagg ccactctaga gtcatcatct ttcctatgca aagagcttcc
721 tgaaggtact ctcatgctcc attacttcca ccctcaccat acggtgggct ttgcaatgct
781 gggaatgatt aaggctgcag gaaagagaat ctatcactta aatgtggaag ttgaacaaat
841 tgaaaatgag aaactctgct ttgatggttc aaacccaagc aattgcagct gtcttacttt
901 ccttatcaaa gaatgtgaaa ctactcagat cacaaaggac aatccacaag gtacttccca
961 agttcctgca gacctcagaa tcagcatcaa caccttctgc agagccttcc ccttccattt
1021 gatgtttgac tctaacatgg tggtccttca gcttggggag ggcctacgga aacaacttcg
1081 atatgacact cacagagcac tgaagtttga ggactgtttt gagatcgtct ctccaatgat
1141 taatgccacc tttgatagag tcttgctacg attgtccact ccatttgtta taaggaccaa
1201 gcccgaagct tctggtactg agaatgaaga taaggtgatg gaaatcaaag gacaaatgat
1261 ccatgtccca gaatcaagtg ccattttgtt tttgggatct ccatgtgtgg acaagttgga
1321 tgaactgatg ggccgaggac tgcatctctc agacatccct attcatgatg ccacccgaga
1381 tgtaatttta gttggagagc aagccaaggc ccaggatggc ttgaagaaga ggatggataa
1441 attaaaggcc acattagaaa gaactcacca ggccctggaa gaagaaaaaa agaagacagt
1501 ggatctgctg tattccattt tccctggtga tgtagcccag cagttgtggc aagggcagca
1561 agtacaggcc agaaagtttg atgatgtcac catgctcttc tctgacattg tgggctttac
1621 agccatatgt gctcagtgta ctcccatgca ggtgatcagc atgctcaatg aactctacac
1681 aagatttgat caccagtgtg gccttttgga tatttacaag gtggaaacaa taggggacgc
1741 atactgtgtg gcatcaggac tgcacaggaa aagcctatgc catgcaaagc ccattgctct
1801 gatggcctta aagatgatgg agctttcaga agaagttctg actcccgatg gaaaagcaat
1861 tcagatgcgg ataggcattc actcaggctc tgtgctggct ggtgtggtcg gagtacgaat
1921 gccacgatat tgtctttttg gaaacaatgt cactctggca agcaaatttg aatctggaag
1981 tcatcctcgg cgtatcaatg tcagccctac tacttaccaa ttactaaaac gagaagacag
2041 tttcacattt attcctcgtt ctcgagaaga acttccagac aactttccga aggaaattcc
2101 tggggtctgc tatttcctgg agttaaggac tgacccaaag ccaccaaagc catccctatc
2161 ttcatcgaga ataaaaaagg tttcctacaa tattggcaca atgttcctcc gggaaacaag
2221 cctctaaggc ctcatgggcc cagctttctt gtac
//