LOCUS BC112105 1692 bp mRNA linear HUM 21-JAN-2006
DEFINITION Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2,
transcript variant 1, mRNA (cDNA clone MGC:138310 IMAGE:8327573),
complete cds.
ACCESSION BC112105
VERSION BC112105.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1692)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1692)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (19-JAN-2006) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 4 Row: h Column: 3.
FEATURES Location/Qualifiers
source 1..1692
/db_xref="H-InvDB:HIT000340234"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:138310 IMAGE:8327573"
/tissue_type="Brain, cerebral cortex, PCR rescued clones"
/clone_lib="NIH_MGC_287"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: CTGATGTT"
gene 1..1692
/gene="PFKFB2"
/gene_synonym="PFK-2/FBPase-2"
/db_xref="GeneID:5208"
/db_xref="HGNC:HGNC:8873"
/db_xref="IMGT/GENE-DB:8873"
/db_xref="MIM:171835"
CDS 53..1570
/gene="PFKFB2"
/gene_synonym="PFK-2/FBPase-2"
/codon_start=1
/product="6-phosphofructo-2-kinase/fructose-2,
6-biphosphatase 2, isoform a"
/protein_id="AAI12106.1"
/db_xref="GeneID:5208"
/db_xref="HGNC:HGNC:8873"
/db_xref="IMGT/GENE-DB:8873"
/db_xref="MIM:171835"
/translation="MSGASSSEQNNNSYETKTPNLRMSEKKCSWASYMTNSPTLIVMI
GLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYKSYDFFRHDNEEAMKIR
KQCALVALEDVKAYLTEENGQIAVFDATNTTRERRDMILNFAEQNSFKVFFVESVCDD
PDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYRPLDPDNYDKDLSFIKV
INVGQRFLVNRVQDYIQSKIVYYLMNIHVQPRTIYLCRHGESEFNLLGKIGGDSGLSV
RGKQFAQALRKFLEEQEITDLKVWTSQLKRTIQTAESLGVPYEQWKILNEIDAGVCEE
MTYAEIEKRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNVLVISHQ
AVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGCKVETIKLNVEAVNTHRDKPT
NNFPKNQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRPLKPLSPLRAQDMQEGAD"
BASE COUNT 459 a 418 c 425 g 390 t
ORIGIN
1 ccaaggcagg gagggatctt aggagagatc gtaggacatc tgaagagctg ccatgtctgg
61 ggcatcttcc tcagaacaga acaacaacag ctatgaaacc aaaaccccaa atcttcgaat
121 gtcagagaag aaatgttcat gggcctccta catgaccaac tccccgactc tgatcgttat
181 gattggtttg ccagcccggg gtaaaaccta cgtgtccaag aaactaacac gctacctcaa
241 ctggattgga gtccccacca aagtgtttaa tcttggggtg tatcggcgtg aagcagtcaa
301 gtcctataag tcctacgact tctttcggca tgacaatgag gaggccatga agatccgcaa
361 acagtgtgct ctggtggcgc tggaagatgt taaggcgtat ctcactgagg agaatggtca
421 gattgcggtg tttgatgcca ccaatacaac ccgggagagg agggacatga ttttgaactt
481 tgctgaacag aattccttca aggtattctt tgtggaatcc gtctgtgatg atcctgatgt
541 cattgctgcc aatattctgg aggttaaggt atcaagccct gactatcctg aaaggaatag
601 agagaacgtg atggaggact tcctgaagag aattgaatgc tacaaagtta cctaccgacc
661 tcttgaccca gacaactatg acaaggatct ttctttcatc aaggtgataa acgtgggcca
721 gcgattttta gtcaacagag tccaggacta catccagagc aagatagtct actacctcat
781 gaatatccac gtccagcctc gcaccattta cctttgccgg catggagaaa gcgagttcaa
841 tctcttgggg aagattgggg gtgactctgg cctctcggtg cggggaaagc agtttgccca
901 agctctaagg aaatttctgg aggaacagga aataacagac ctcaaagtgt ggacaagcca
961 gttgaagagg accatacaga ctgctgaatc tctcggggtg ccctatgagc agtggaagat
1021 tctgaatgag attgatgctg gtgtgtgtga agagatgacc tatgcagaga ttgagaaacg
1081 gtacccagaa gagtttgcac ttcgagatca agagaagtat ctgtatcgat atcctggtgg
1141 ggagtcatac caggacctgg tgcagcggct ggagcctgtc atcatggagc tggaacgtca
1201 gggcaatgtc ctcgtcatct cccaccaggc tgtcatgcgc tgcctcctgg cctacttctt
1261 ggataagggc gcagatgagc taccatactt gagatgccct ctccatacca tcttcaaact
1321 tactcctgtg gcctatgggt gcaaagtgga aacaattaaa cttaacgtgg aggctgtgaa
1381 cacgcaccgt gacaagccaa ctaacaactt ccccaagaac caaacccctg taaggatgag
1441 aaggaacagc tttacgcctc tgtccagttc gaatacaata aggcgtccaa gaaattacag
1501 tgttgggagc cggcccctca agcccctcag ccctctccgt gcccaggaca tgcaagaagg
1561 ggccgactag ccgaagaccc aagtcagcat tccggtggtg taactgtgtg tttccctcca
1621 gccctggcct cctgcccttg tcactaatca ccaaggagtc attaacttcc tccctctatg
1681 cccacccctg ac
//