LOCUS BC075076 1586 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2,
mRNA (cDNA clone MGC:104138 IMAGE:30915594), complete cds.
ACCESSION BC075076
VERSION BC075076.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1586)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1586)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-JUN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 4, 2004 this sequence version replaced BC075076.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Genome Sequence Centre, British Columbia Cancer
Center
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRBU Plate: 5 Row: D Column: 7.
FEATURES Location/Qualifiers
source 1..1586
/db_xref="H-InvDB:HIT000265583"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:104138 IMAGE:30915594"
/tissue_type="Brain, PCR rescued clones"
/clone_lib="NIH_MGC_272"
/lab_host="DH10B"
/note="Vector: pCR4 Topo TA with reversed insert"
gene 1..1586
/gene="PFKFB2"
/gene_synonym="PFK-2/FBPase-2"
/db_xref="GeneID:5208"
/db_xref="HGNC:HGNC:8873"
/db_xref="MIM:171835"
CDS 48..1565
/gene="PFKFB2"
/gene_synonym="PFK-2/FBPase-2"
/codon_start=1
/product="6-phosphofructo-2-kinase/fructose-2,
6-biphosphatase 2"
/protein_id="AAH75076.1"
/db_xref="GeneID:5208"
/db_xref="HGNC:HGNC:8873"
/db_xref="MIM:171835"
/translation="MSGASSSEQNNNSYETKTPNLRMSEKKCSWASYMTNSPTLIVMI
GLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYKSYDFFRHDNEEAMKIR
KQCALVALEDVKAYLTEENGQIAVFDATNTTRERRDMILNFAEQNSFKVFFVESVCDD
PDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYRPLDPDNYDKDLSFIKV
INVGQRFLVNRVQDYIQSKIVYYLMNIHVQPRTIYLCRHGESEFNLLGKIGGDSGLSV
RGKQFAQALRKFLEEQEITDLKVWTSQLKRTIQTAESLGVPYEQWKILNEIDAGVCEE
MTYAEIEKRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNVLVISHQ
AVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGCKVETIKLNVEAVNTHRDKPT
NNFPKNQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRPLKPLSPLRAQDMQEGAD"
BASE COUNT 441 a 378 c 406 g 361 t
ORIGIN
1 gcagggaggg atcttaggag agatcgtagg acatctgaag agctgccatg tctggggcat
61 cttcctcaga acagaacaac aacagctatg aaaccaaaac cccaaatctt cgaatgtcag
121 agaagaaatg ttcatgggcc tcctacatga ccaactcccc gactctgatc gttatgattg
181 gtttgccagc ccggggtaaa acctacgtgt ccaagaaact aacacgctac ctcaactgga
241 ttggagtccc caccaaagtg tttaatcttg gggtgtatcg gcgtgaagca gtcaagtcct
301 ataagtccta cgacttcttt cggcatgaca atgaggaggc catgaagatc cgcaaacagt
361 gtgctctggt ggcgctggaa gatgttaagg cgtatctcac tgaggagaat ggtcagattg
421 cggtgtttga tgccaccaat acaacccggg agaggaggga catgattttg aactttgctg
481 aacagaattc cttcaaggta ttctttgtgg aatccgtctg tgatgatcct gatgtcattg
541 ctgccaatat tctggaggtt aaggtatcaa gccctgacta tcctgaaagg aacagagaga
601 acgtgatgga ggacttcctg aagagaattg aatgctacaa agttacctac cgacctcttg
661 acccagacaa ctatgacaag gatctttctt tcatcaaggt gataaacgtg ggccagcgat
721 ttttagtcaa cagagtccag gactacatcc agagcaagat agtctactac ctcatgaata
781 tccacgtcca gcctcgcacc atttaccttt gccggcatgg agaaagcgag ttcaatctct
841 tggggaagat tgggggtgac tctggcctct cggtgcgggg aaagcagttt gcccaagctc
901 taaggaaatt tctggaggaa caggaaataa cagacctcaa agtgtggaca agccagttga
961 agaggaccat acagactgct gaatctctcg gggtgcccta tgagcagtgg aagattctga
1021 atgagattga tgctggtgtg tgtgaagaga tgacctatgc agagattgag aaacggtacc
1081 cagaagagtt tgcacttcga gatcaagaga agtatctgta tcgatatcct ggtggggagt
1141 cataccagga cctggtgcag cggctggagc ctgtcatcat ggagctggaa cgtcagggca
1201 atgtcctcgt catctcccac caggctgtca tgcgctgcct cctggcctac ttcttggata
1261 agggcgcaga tgagctacca tacttgagat gccctctcca taccatcttc aaacttactc
1321 ctgtggccta tgggtgcaaa gtggaaacaa ttaaacttaa cgtggaggct gtgaacacgc
1381 accgtgacaa gccaactaac aacttcccca agaaccaaac ccctgtaagg atgagaagga
1441 acagctttac gcctctgtcc agttcgaata caataaggcg tccaagaaat tacagtgttg
1501 ggagccggcc cctcaagccc ctcagccctc tccgtgccca ggacatgcaa gaaggggccg
1561 actagccgaa gacccaagtc agcatt
//