LOCUS BC030679 1452 bp mRNA linear ROD 07-OCT-2003
DEFINITION Mus musculus CD8 antigen, alpha chain, mRNA (cDNA clone MGC:41580
IMAGE:1247019), complete cds.
ACCESSION BC030679
VERSION BC030679.1
KEYWORDS MGC.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 1452)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1452)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (20-MAY-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Marcello Bento Soares, Ph.D.
cDNA Library Preparation: M. Bento Soares, University of Iowa
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 66 Row: h Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1452
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="taxon:10090"
/clone="MGC:41580 IMAGE:1247019"
/tissue_type="Mammary gland"
/clone_lib="Soares_mammary_gland_NbMMG"
/lab_host="DH10B"
/note="Vector: pT7T3-Pac"
gene 1..1452
/gene="Cd8a"
/gene_synonym="Ly-B"
/db_xref="GeneID:12525"
/db_xref="MGI:MGI:88346"
CDS 111..734
/gene="Cd8a"
/gene_synonym="Ly-B"
/codon_start=1
/product="Cd8a protein"
/protein_id="AAH30679.1"
/db_xref="GeneID:12525"
/db_xref="MGI:MGI:88346"
/translation="MDAELGQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMA
SSHNKITWDEKLNSSKLFSAMRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSV
VPVLQKVNSTTTKPVLRTPSPVHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAP
LAGICVALLLSLIITLICYHRSRKRVCKCPRPLVRQEGKPRPSEKIV"
misc_feature 117..416
/gene="Cd8a"
/gene_synonym="Ly-B"
/note="IG; Region: Immunoglobulin"
/db_xref="CDD:smart00409"
BASE COUNT 381 a 368 c 346 g 357 t
ORIGIN
1 cgttgacccg ctttctgtcg ctgaacctgc tgctgctggg tgagtcgatt atcctgggga
61 gtggagaagc taagccacag gcacccgaac tccgaatctt tccaaagaaa atggacgccg
121 aacttggtca gaaggtggac ctggtatgtg aagtgttggg gtccgtttcg caaggatgct
181 cttggctctt ccagaactcc agctccaaac tcccccagcc caccttcgtt gtctatatgg
241 cttcatccca caacaagata acgtgggacg agaagctgaa ttcgtcgaaa ctgttttctg
301 ccatgaggga cacgaataat aagtacgttc tcaccctgaa caagttcagc aaggaaaacg
361 aaggctacta tttctgctca gtcatcagca actcggtgat gtacttcagt tctgtcgtgc
421 cagtccttca gaaagtgaac tctactacta ccaagccagt gctgcgaact ccctcacctg
481 tgcaccctac cgggacatct cagccccaga gaccagaaga ttgtcggccc cgtggctcag
541 tgaaggggac cggattggac ttcgcctgtg atatttacat ctgggcaccc ttggccggaa
601 tctgcgtggc ccttctgctg tccttgatca tcactctcat ctgctaccac aggagccgaa
661 agcgtgtttg caaatgtccc aggccgctag tcagacagga aggcaagccc agaccttcag
721 agaaaattgt gtaaaatggc accgccagga agctacaact actacatgac ttcagatctc
781 ttcttgcaag aggccaggcc ctcctttttc aagtttcctg ctgtcttatg tattgccctc
841 tgtattgttt tagtaggggt gtgatgggga cagttccttt ttctttatga attctctttg
901 acacaaagca tacttgtatg catacaatgg gagtaatgag cagactgtaa caccagagct
961 agttccagtt tcggggtcca tgtcgctggt ggcctcagca cccacttgat ataaatctcc
1021 tgtctgccca tcatatagaa gaagctgaag atcagaggtg gaaacagcag gatctgtaga
1081 cccggagaga acccaagcta gaggaaccct cactgactgg tgcagggatc tcacccccat
1141 cccctgagct ctctgtttag gtatgtgtct ttagtatagc atgcttgtgc ctcaaactgc
1201 aaggaagcaa gtggtatgaa tctgtgatct gctcagctct ggctgtgagc atcagatgta
1261 acaaatcaaa gtcaggagaa ctgcctttag cggtgcacct ggacatcaga gccccttgta
1321 agaactggga aattgctggc agtctagtgg agcggtacgg taaatctgga aaacactccc
1381 ctctgtcact gaaggctgta gtagaatcca attaaagcta ttcaaaccac aaaaaaaaaa
1441 aaaaaaaaaa aa
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