LOCUS AY568055 10459 bp DNA circular SYN 21-NOV-2011
DEFINITION Cloning vector pAGRIKOLA, complete sequence.
ACCESSION AY568055
VERSION AY568055.1
KEYWORDS .
SOURCE Cloning vector pAGRIKOLA
ORGANISM Cloning vector pAGRIKOLA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 10459)
AUTHORS Hilson,P., Allemeersch,J., Altmann,T., Aubourg,S., Avon,A.,
Beynon,J., Bhalerao,R.P., Bitton,F., Caboche,M., Cannoot,B.,
Chardakov,V., Cognet-Holliger,C., Colot,V., Crowe,M., Darimont,C.,
Durinck,S., Eickhoff,H., de Longevialle,A.F., Farmer,E.E.,
Grant,M., Kuiper,M.T., Lehrach,H., Leon,C., Leyva,A., Lundeberg,J.,
Lurin,C., Moreau,Y., Nietfeld,W., Paz-Ares,J., Reymond,P.,
Rouze,P., Sandberg,G., Segura,M.D., Serizet,C., Tabrett,A.,
Taconnat,L., Thareau,V., Van Hummelen,P., Vercruysse,S.,
Vuylsteke,M., Weingartner,M., Weisbeek,P.J., Wirta,V.,
Wittink,F.R., Zabeau,M. and Small,I.
TITLE Versatile gene-specific sequence tags for Arabidopsis functional
genomics: transcript profiling and reverse genetics applications
JOURNAL Genome Res. 14 (10B), 2176-2189 (2004)
PUBMED 15489341
REFERENCE 2 (bases 1 to 10459)
AUTHORS Small,I.D. and Hilson,P.
TITLE pAGRIKOLA sequence
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 10459)
AUTHORS Small,I.D. and Hilson,P.
TITLE Direct Submission
JOURNAL Submitted (07-MAR-2004) URGV, INRA/CNRS/UEVE, 2, rue Gaston
Cremieux, CP5708, Evry, Essonne 91057, France
FEATURES Location/Qualifiers
source 1..10459
/organism="Cloning vector pAGRIKOLA"
/mol_type="other DNA"
/db_xref="taxon:267887"
/lab_host="Escherichia coli"
/note="high-throughput plant RNAi cloning vector"
rep_origin 18..672
/note="colE1"
gene complement(820..1635)
/gene="nptI"
CDS complement(820..1635)
/gene="nptI"
/EC_number="2.7.1.95"
/note="aminoglycoside 3'-phosphotransferase; confers
kanamycin resistance in bacteria"
/codon_start=1
/transl_table=11
/product="APH(3')I"
/protein_id="AAS75425.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 1925..2472
/note="pSa"
repeat_region 2488..2512
/note="T-DNA left border"
regulatory complement(2532..2791)
/regulatory_class="polyA_signal_sequence"
/note="nos terminator"
gene complement(2806..3357)
/gene="bar"
CDS complement(2806..3357)
/gene="bar"
/note="phospinothricin N-acetyltransferase; confers Basta
resistance in plants"
/codon_start=1
/transl_table=11
/product="BAR"
/protein_id="AAS75426.1"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQ
EWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGL
GSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHD
VGFWQLDFSLPVPPRPVLPVTEI"
regulatory complement(3378..3661)
/regulatory_class="promoter"
/note="nos promoter"
regulatory 3980..5317
/regulatory_class="promoter"
/note="CaMV 35S promoter"
misc_feature 5322..5446
/note="attR1 recombination site"
gene complement(5875..6180)
/gene="ccdB"
CDS complement(5875..6180)
/gene="ccdB"
/note="cytotoxin; counterselection of Gateway cassette"
/codon_start=1
/transl_table=11
/product="CCDB"
/protein_id="AAS75427.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
misc_feature complement(6619..6738)
/note="attR2 recombination site"
intron 6766..7544
/note="intron from Flaveria trinerva PDK gene encoding
pyruvate orthophosphate dikinase"
intron complement(7581..7763)
/note="intron from Ricinus communis CAT1 gene encoding
catalase"
misc_feature 7791..7910
/note="attR2 recombination site"
gene complement(8239..8544)
/gene="ccdB"
CDS complement(8239..8544)
/gene="ccdB"
/note="cytotoxin; counterselection of Gateway cassette"
/codon_start=1
/transl_table=11
/product="CCDB"
/protein_id="AAS75428.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
misc_feature complement(9075..9199)
/note="attR1 recombination site"
regulatory 9208..9915
/regulatory_class="polyA_signal_sequence"
/note="ocs terminator"
repeat_region 10404..10429
/note="T-DNA right border"
BASE COUNT 2994 a 2394 c 2261 g 2810 t
ORIGIN
1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
1441 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
2221 aacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
2461 ggatttccga tccccggaat tagatcttgg caggatatat tgtggtgtaa cgttatcagc
2521 ttgcatgccg gtcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
2581 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
2641 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
2701 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
2761 ctttattgcc aaatgtttga acgatctgct tgactctagg ggtcatcaga tttcggtgac
2821 gggcaggacc ggacggggcg gcaccggcag gctgaagtcc agctgccaga aacccacgtc
2881 atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag
2941 cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt
3001 gaagccctgt gcctccaggg acttcagcag gtgggtgtag agcgtggagc ccagtcccgt
3061 ccgctggtgg cggggggaga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg
3121 tgccttccag ggacccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag
3181 ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg
3241 ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc
3301 cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatggt
3361 agatcccctc gatcgagttg agagtgaata tgagactcta attggatacc gaggggaatt
3421 tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag
3481 gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag
3541 aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg
3601 gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat
3661 cgataacatt aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg
3721 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
3781 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
3841 tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt
3901 cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
3961 agcggccgct cgacgaatta attccaatcc cacaaaaatc tgagcttaac agcacagttg
4021 ctcctctcag agcagaatcg ggtattcaac accctcatat caactactac gttgtgtata
4081 acggtccaca tgccggtata tacgatgact ggggttgtac aaaggcggca acaaacggcg
4141 ttcccggagt tgcacacaag aaatttgcca ctattacaga ggcaagagca gcagctgacg
4201 cgtacacaac aagtcagcaa acagacaggt tgaacttcat ccccaaagga gaagctcaac
4261 tcaagcccaa gagctttgct aaggccctaa caagcccacc aaagcaaaaa gcccactggc
4321 tcacgctagg aaccaaaagg cccagcagtg atccagcccc aaaagagatc tcctttgccc
4381 cggagattac aatggacgat ttcctctatc tttacgatct aggaaggaag ttcgaaggtg
4441 aaggtgacga cactatgttc accactgata atgagaaggt tagcctcttc aatttcagaa
4501 agaatgctga cccacagatg gttagagagg cctacgcagc aggtctcatc aagacgatct
4561 acccgagtaa caatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca
4621 aaagattcag gactaattgc atcaagaaca cagagaaaga catatttctc aagatcagaa
4681 gtactattcc agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga
4741 ttggagtctc taaaaaggta gttcctactg aatctaaggc catgcatgga gtctaagatt
4801 caaatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat acagagtctt
4861 ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga ccctctggtc
4921 taccccaaaa atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
4981 caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
5041 gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag
5101 gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
5161 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac
5221 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
5281 atataaggaa gttcatttca tttggagagg acacgctcga gacaagtttg tacaaaaaag
5341 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
5401 aacagactac ataatactgt aaaacacaac atatccagtc actatgaatc aactacttag
5461 atggtattag tgacctgtag tcgaccgaca gccttccaaa tgttcttcgg gtgatgctgc
5521 caacttagtc gaccgacagc cttccaaatg ttcttctcaa acggaatcgc cgtatccagc
5581 ctactcgcta ttgtcctcaa tgccgtatta aatcataaaa agaaataaga aaaagaggtg
5641 cgagcctctt ttttgtgtga caaaataaaa acatctacct attcatatac gctagtgtca
5701 tagtcctgaa aatcatctgc atcaagaaca atttcacaac tcttatactt ttctcttaca
5761 agtcgttcgg cttcatctgg attttcagcc tctatactta ctaaacgtga taaagtttct
5821 gtaatttcta ctgtatcgac ctgcagactg gctgtgtata agggagcctg acatttatat
5881 tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc
5941 cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca
6001 gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag
6061 acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg
6121 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat
6181 ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct
6241 cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat
6301 tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg
6361 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac
6421 atacttcggg tgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
6481 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
6541 tattcggcgc aaagtgcgtc gggtgatgct gccaacttag tcgactacag gtcactaata
6601 ccatctaagt agttgattca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
6661 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
6721 tcagctttct tgtacaaagt ggtctcgagg aattcggtac cccagcttgg taaggaaata
6781 attattttct tttttccttt tagtataaaa tagttaagtg atgttaatta gtatgattat
6841 aataatatag ttgttataat tgtgaaaaaa taatttataa atatattgtt tacataaaca
6901 acatagtaat gtaaaaaaat atgacaagtg atgtgtaaga cgaagaagat aaaagttgag
6961 agtaagtata ttatttttaa tgaatttgat cgaacatgta agatgatata ctagcattaa
7021 tatttgtttt aatcataata gtaattctag ctggtttgat gaattaaata tcaatgataa
7081 aatactatag taaaaataag aataaataaa ttaaaataat atttttttat gattaatagt
7141 ttattatata attaaatatc tataccatta ctaaatattt tagtttaaaa gttaataaat
7201 attttgttag aaattccaat ctgcttgtaa tttatcaata aacaaaatat taaataacaa
7261 gctaaagtaa caaataatat caaactaata gaaacagtaa tctaatgtaa caaaacataa
7321 tctaatgcta atataacaaa gcgcaagatc tatcaatttt atatagtatt atttttcaat
7381 caacattctt attaatttct aaataatact tgtagtttta ttaacttcta aatggattga
7441 ctattaatta aatgaattag tcgaacatga ataaacaagg taacatgata gatcatgtca
7501 ttgtgttatc attgatctta catttggatt gattacagtt gggaagctgg gttcgaaatc
7561 gataagcttg cgctgcagtt atcatcatca tcatagacac acgaaataaa gtaatcagat
7621 tatcagttaa agctatgtaa tatttacacc ataaccaatc aattaaaaaa tagatcagtt
7681 taaagaaaga tcaaagctca aaaaaataaa aagagaaaag ggtcctaacc aagaaaatga
7741 aggagaaaaa ctagaaattt acctgcacaa gcttggatcc tctagaccac tttgtacaag
7801 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
7861 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg aatcaactac
7921 ttagatggta ttagtgacct gtagtcgact aagttggcag catcacccga cgcactttgc
7981 gccgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct
8041 gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggatttca
8101 caactcttat acttttctct tacaagtcgt tcggcttcat ctggattttc agcctctata
8161 cttactaaac gtgataaagt ttctgtaatt tctactgtat cgacctgcag actggctgtg
8221 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
8281 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
8341 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
8401 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
8461 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
8521 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt
8581 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt
8641 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga
8701 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg
8761 cttcatagca tacctctttt tgacatactt cgggtatgat gcagatgatt ttcaggacta
8821 tgacactagc gtatatgaat aggtagatgt ttttattttg tcacacaaaa aagaggctcg
8881 cacctctttt tcttatttct ttttatgatt taatacggca ttgaggacaa tagcgagtag
8941 gctggatacg acgattccgt ttgagaagaa catttggaag gctgtcggtc gactaagttg
9001 gcagcatcac ccgaagaaca tttggaaggc tgtcggtcga ctacaggtca ctaataccat
9061 ctaagtagtt gattcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
9121 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
9181 cttttttgta caaacttgtc tagagtcctg ctttaatgag atatgcgaga cgcctatgat
9241 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
9301 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
9361 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
9421 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
9481 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
9541 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
9601 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
9661 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
9721 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
9781 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
9841 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
9901 gtcgaggctc agcaggacct gcaggcatgc aagctagctt actagtgatg catattctat
9961 agtgtcacct aaatctgcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
10021 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
10081 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
10141 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
10201 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
10261 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
10321 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
10381 acgcaggaaa gaacatgaag gccttgacag gatatattgg cgggtaaact aagtcgctgt
10441 atgtgtttgt ttgagatct
//