LOCUS AY343322 2469 bp DNA linear BCT 13-AUG-2003
DEFINITION Cellulomonas uda cellobiose phosphorylase gene, complete cds.
ACCESSION AY343322
VERSION AY343322.1
KEYWORDS .
SOURCE Cellulomonas uda
ORGANISM Cellulomonas uda
Bacteria; Actinobacteria; Micrococcales; Cellulomonadaceae;
Cellulomonas.
REFERENCE 1 (bases 1 to 2469)
AUTHORS Griessler,R., Schwarz,A., Splechtna,B. and Nidetzky,B.
TITLE Cellobiose phosphorylase from Cellulomonas uda: Gene cloning and
expression in E. coli, and application of the recombinant enzyme in
a novel glycosynthase-type reaction
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2469)
AUTHORS Griessler,R., Schwarz,A., Splechtna,B. and Nidetzky,B.
TITLE Direct Submission
JOURNAL Submitted (16-JUL-2003) Bio/Technology, Institute of Biotechnology,
Graz University of Technology, Petersgasse 12, Graz A-8010, Austria
FEATURES Location/Qualifiers
source 1..2469
/organism="Cellulomonas uda"
/mol_type="genomic DNA"
/strain="DSM 20108"
/db_xref="taxon:1714"
CDS 1..2469
/EC_number="2.4.1.20"
/note="glycosyltransferase; cellobiose:orthophosphate
alpha-D-glucosyl transferase"
/codon_start=1
/transl_table=11
/product="cellobiose phosphorylase"
/protein_id="AAQ20920.1"
/translation="MRYGHFDDEAREYVITTPHTPYPWINYLGSEQFFSLLSHQAGGY
SFYRDAKMRRLTRYRYNNIPADAGGRYLYVNDGGDVWTPSWLPVKADLDHFEARHGLG
YSTITGERNGVRVETLFFVPVGENAEVQKVTVTNTSDSYKSLTLFSFVEFCLWNAQDD
QTNYQRNLSIGEVEVEQESPHGSAIYHRTEYRERRDHYAVFAVNTQAEGFDTDRDTFV
GAYNSLGEAAVPLKGESANSVASGWYPIGSHSVAVSLAPGESRELVYVLGYVENPDEE
KWADDAKQVVNKERAHALLSRFATSEQTDAAFAALKDYWTDLLSTYSVSSNDEKLDRM
VNIWNQYQCMVTFNMSRSASFFETGIGRGMGFRDSNQDLLGFVHLIPERARERIIDIA
STQFADGSAYHQYQPLTKRGNNDIGSGFNDDPLWLIAGTAAYIKETGDFSILDEPVPF
DNEPGSEVPLFEHLTRSFEFTVTHRGPHGLPLIGRADWNDCLNLNCFSTTPGESFQTT
ENQAGGVAESTFIAAQFVLYGEQYAELAARRGLADVADRARGHVAEMRDALLTDGWDG
SWFLRAYDYYGNPIGTDAHDEGKIWIEPQGFAVMAGVGVGEGPQDTDAPAIKALDSVN
EMLATDHGMVLQYPAYTTYQVHMGEVSTYPPGYKENGGIFCHNNPWVIIAETVVGRGG
RAFDYYKRITPAYREDISDVHRLEPYVYAQMIAGKEAVRHGEAKNSWLTGTAAWNFVT
VSQYLLGVRPEYDGLVVDPQIGPDVPSFTVTRVARGATYEITVTNSGTDGSRGRLVVD
GTPVEGNLVPYAPAGSTVRVDVTL"
BASE COUNT 419 a 882 c 809 g 359 t
ORIGIN
1 atgcgttacg ggcacttcga cgacgaggca cgcgagtacg tcatcacgac gccgcacacc
61 ccgtacccgt ggatcaacta cctgggctcc gagcagttct tctcgctgct gtcgcaccag
121 gccggcgggt actcgttcta ccgggacgcc aagatgcgtc gcctcacgcg ctaccggtac
181 aacaacatcc ccgccgacgc gggcggccgc tacctgtacg tcaacgacgg cggcgacgtg
241 tggaccccgt cgtggctgcc ggtcaaggcg gacctcgacc acttcgaggc gcgccacggc
301 ctgggctact cgaccatcac gggcgagcgg aacggcgtgc gcgtcgagac gctgttcttc
361 gtgccggtcg gcgagaacgc cgaggtgcag aaggtcaccg tcaccaacac gtccgactcc
421 tacaagtcgc tcacgctgtt ctcgttcgtc gagttctgcc tgtggaacgc gcaggacgac
481 cagacgaact accagcgcaa cctgtcgatc ggcgaggtcg aggtcgagca ggagagcccg
541 cacggctccg cgatctacca ccgcaccgag taccgcgagc gccgcgacca ctacgcggtg
601 ttcgccgtga acacccaggc cgagggcttc gacaccgacc gcgacacgtt cgtcggcgcg
661 tacaactcgc tcggcgaggc cgcggttccg ctcaagggcg agtccgcgaa ctcggtcgcg
721 tccggctggt acccgatcgg ctcgcactcc gtcgcggtgt ccctcgcccc cggcgagtcg
781 cgcgagctcg tctacgtcct cggctacgtc gagaaccccg acgaggagaa gtgggccgac
841 gacgccaagc aggtcgtcaa caaggagcgc gcgcacgcgc tgctgtcccg gttcgcgacc
901 tccgagcaga cggacgcggc gttcgcggcg ctcaaggact actggaccga cctgctgtcg
961 acgtactcgg tgagctcgaa cgacgagaag ctcgaccgga tggtcaacat ctggaaccag
1021 taccagtgca tggtcacgtt caacatgtcg cgctcggcgt cgttcttcga gaccgggatc
1081 ggccgcggca tgggcttccg cgactccaac caggacctgc tgggcttcgt ccacctgatc
1141 ccggagcggg cgcgcgagcg catcatcgac atcgcctcga cgcagttcgc ggacggctcg
1201 gcgtaccacc agtaccagcc gctgacgaag cgcgggaaca acgacatcgg ctcgggcttc
1261 aacgacgacc cgctgtggct gatcgcgggc accgcggcgt acatcaagga gaccggcgac
1321 ttctcgatcc tcgacgagcc ggtgccgttc gacaacgagc ccggctccga ggtcccgctg
1381 ttcgagcacc tgacgcgctc gttcgagttc acggtcacgc accgcggccc gcacggcctg
1441 ccgctgatcg gccgcgccga ctggaacgac tgcctcaacc tcaactgctt ctccacgacg
1501 cccggtgagt cgttccagac caccgagaac caggcgggcg gggtcgcgga gtccacgttc
1561 atcgccgcgc agttcgtcct gtacggcgag cagtacgccg agctcgcggc gcgccgcggc
1621 ctggccgacg tcgccgaccg ggcgcgcggc cacgtcgccg agatgcgcga cgcgctgctc
1681 accgacggct gggacggctc ctggttcctg cgcgcgtacg actactacgg caacccgatc
1741 gggaccgacg cgcacgacga gggcaagatc tggatcgagc cgcaaggctt cgccgtcatg
1801 gcgggcgtcg gcgtcggcga gggcccgcag gacacggacg cccccgcgat caaggcgctc
1861 gactcggtca acgagatgct cgcgaccgac cacggcatgg tgctccagta ccccgcgtac
1921 accacgtacc aggtgcacat gggcgaggtc tccacgtacc cgccggggta caaggagaac
1981 ggcggcatct tctgccacaa caacccgtgg gtgatcatcg ccgagacggt cgtgggccgc
2041 ggtgggcgcg cgttcgacta ctacaagcgc atcacgcccg cctaccgcga ggacatctcc
2101 gacgtccacc gcctcgagcc gtacgtgtac gcgcagatga tcgcgggcaa ggaggcggtc
2161 cgccacggcg aggccaagaa ctcctggctc acgggcaccg ctgcgtggaa cttcgtcacg
2221 gtctcgcagt acctgctggg tgtgcggccg gagtacgacg ggctcgtcgt cgacccgcag
2281 atcggcccgg acgtgccgtc gttcaccgtc acgcgcgtcg cgcgcggggc gacgtacgag
2341 atcacggtga cgaactcggg caccgacggc tcgcgcggcc ggctggtcgt ggacggcacg
2401 ccggtcgagg gcaacctcgt gccgtacgcc cccgccgggt cgacggtccg cgtcgacgtg
2461 accctctag
//