LOCUS AY072794 2884 bp DNA linear BCT 27-JAN-2002
DEFINITION Clostridium thermocellum YM4 cellobiose phosphorylase (cbp) gene,
complete cds.
ACCESSION AY072794
VERSION AY072794.1
KEYWORDS .
SOURCE Acetivibrio thermocellus
ORGANISM Acetivibrio thermocellus
Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
Acetivibrio.
REFERENCE 1 (bases 1 to 2884)
AUTHORS Kim,Y.-K., Kitaoka,M., Mori,Y. and Hayashi,K.
TITLE Cellobiose phosphorylase of Clostridium thermocellum YM4
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2884)
AUTHORS Kim,Y.-K., Kitaoka,M., Mori,Y. and Hayashi,K.
TITLE Direct Submission
JOURNAL Submitted (10-JAN-2002) Enzyme Lab., Natl. Food Res. Inst., 2-1-12
Kannondai, Tsukuba, Ibaraki 305-8642, Japan
FEATURES Location/Qualifiers
source 1..2884
/organism="Acetivibrio thermocellus"
/mol_type="genomic DNA"
/strain="YM4"
/db_xref="taxon:1515"
gene 291..2726
/gene="cbp"
CDS 291..2726
/gene="cbp"
/EC_number="2.4.1.20"
/codon_start=1
/transl_table=11
/product="cellobiose phosphorylase"
/protein_id="AAL67138.1"
/translation="MKFGFFDDANKEYVITVPRTPYPWINYLGTENFFSLISNTAGGY
CFYRDARLRRITRYRYNNVPIDMGGRYFYIYDNGDFWSPGWSPVKRELESYECRHGLG
YTKIAGKRNGIKAEVTFFVPLNYNGEVQKLILKNEGQDKKKITLFSFIEFCLWNAYDD
MTNFQRNFSTGEVEIEGSVIYHKTEYRERRNHYAFYSVNAKISGFDSDRDSFIGLYNG
FDAPQAVVNGKSNNSVADGWAPIASHSIEIELNPGEQKEYVFIIGYVENKDEEKWESK
GVINKKKAYEMIEQFNTVEKVDKAFEELKSYWNALLSKYFLESHDEKLNRMVNIWNQY
QCMVTFNMSRSASYFESGIGRGMGFRDSNQDLLGFVHQIPERARERLLDLAATQLEDG
SAYHQYQPLTKKGNNEIGSNFNDDPLWLILATAAYIKETGDYSILKEQVPFNNDPSKA
DTMFEHLTRSFYHVVNNLGPHGLPLIGRADWNDCLNLNCFSTVPDESFQTTTSKDGKV
AESVMIAGMFVFIGKDYVKLCEYMGLEEEARKAQQHIDAMKEAILKYGYDGEWFLRAY
DDFGRKVGSKENEEGKIFIESQGFCVMAEIGLEDGKALKALDSVKKYLDTPYGLVLQN
PAFTRYYIEYGEISTYPPGYKENAGIFCHNNAWIICAETVVGRGDMAFDYYRKIAPAY
IEDVSDIHKLEPYVYAQMVAGKDAKRHGEAKNSWLTGTAAWNFVAISQWILGVKPDYD
GLKIDPCIPKAWDGYKVTRYFRGSTYEITVKNPNHVSKGVAKITVDGNEISGNILPVF
NDGKTHKVEVIMG"
BASE COUNT 964 a 513 c 665 g 742 t
ORIGIN
1 atatcaaaat gcccacttga attaatatgg taaagtagat ataatatttg gtaaacatgc
61 cttcagcaag gttagattag ctgttccgta taaattaacc gtatggtaaa acggcagtca
121 gaaaaataag tcataagatt ccgttatgaa aatatacttc ggtagttaat aataagagat
181 atgaggtaag agatacaaga taagagatat aaggtacgaa tgtataagat ggtgctttta
241 ggcacactaa ataaaaaaca aataaacgaa aattttaagg aggacgaaag atgaagttcg
301 gtttttttga tgatgcaaac aaagagtacg ttattaccgt gcccaggaca ccgtatccgt
361 ggataaacta cctgggtaca gagaatttct tctcactcat ttcgaatacc gcaggcggat
421 attgctttta cagggatgca aggcttagac gtataacaag atacagatac aacaatgttc
481 ctattgacat gggaggacgt tatttctaca tatatgacaa cggtgatttc tggtcgccgg
541 gatggtctcc ggtaaaaagg gagcttgaaa gctatgaatg cagacatgga ctgggatata
601 caaaaattgc cggtaaaaga aacggaataa aagcggaggt cactttcttc gttccgttaa
661 actacaatgg tgaagtccaa aagcttatat tgaagaatga aggacaggac aaaaagaaaa
721 taactctctt ctcttttatt gagttctgct tgtggaatgc ttatgatgat atgaccaact
781 tccagagaaa cttcagcacc ggtgaagttg agattgaagg ctcggttatc tatcacaaga
841 cagagtacag agagcgcaga aaccattacg cattctattc tgtaaatgca aaaatcagcg
901 gatttgacag tgacagagac agcttcatag gactttacaa cggttttgac gctcctcagg
961 ctgtagtgaa cggcaagtca aacaattccg ttgcggacgg atgggcaccg attgcgtccc
1021 acagcattga aattgaattg aatcccgggg agcaaaagga atatgtattt attataggtt
1081 atgtggagaa caaagatgaa gaaaaatggg agtcaaaagg tgtcatcaac aagaaaaaag
1141 cttatgaaat gatagagcag ttcaacactg ttgaaaaggt tgacaaagca tttgaagaac
1201 tcaagagcta ttggaatgct cttctttcaa aatactttct tgaaagccac gatgaaaaac
1261 tcaaccgtat ggttaatata tggaatcagt accagtgtat ggttacattc aacatgtcaa
1321 gaagcgcttc atactttgaa tccggtatcg gaagaggtat gggtttcaga gattcaaacc
1381 aggacttgct gggatttgta caccagatac ccgaaagagc aagagaaagg cttcttgacc
1441 tggctgcaac tcagcttgaa gatggcagtg cgtaccatca gtatcagcct cttaccaaaa
1501 aaggtaacaa tgaaatcgga agcaacttca acgatgaccc gttgtggctg attcttgcaa
1561 ctgctgcata tattaaggaa accggtgatt attcaatact gaaggagcaa gttccgttca
1621 acaatgatcc gtccaaagcc gacaccatgt ttgaacattt gacccgttcc ttctaccatg
1681 tggtaaacaa ccttggacct cacggattgc cgcttatagg tagggcggac tggaatgact
1741 gccttaactt aaactgcttc tccaccgttc cggatgagtc gttccagacc acaacaagca
1801 aagacggaaa agtggcagag tcagttatga ttgccggaat gtttgtgttc atcggaaaag
1861 actatgtgaa gctttgcgaa tacatgggcc ttgaagagga agccaggaaa gctcagcagc
1921 atattgacgc aatgaaggaa gcaattctca aatacggtta tgacggtgag tggttcttaa
1981 gagcttacga cgactttgga agaaaagtcg gaagcaaaga aaacgaagag ggtaagattt
2041 tcattgagtc tcagggattc tgtgtaatgg ctgaaatcgg gcttgaagac ggcaaggctt
2101 tgaaggctct ggattctgtc aagaaatatc ttgacactcc atatggtctt gtacttcaaa
2161 atcccgcgtt tacaagatac tatattgagt acggagaaat ttcaacatat ccaccgggat
2221 acaaagaaaa tgccggtata ttctgccaca acaatgcatg gataatctgt gctgaaacgg
2281 ttgtcggaag aggagacatg gcgtttgatt actatagaaa aatagcacct gcttatattg
2341 aagatgtaag tgacatccac aagcttgagc cttatgttta tgcacagatg gttgccggaa
2401 aagacgcaaa acgccatgga gaagctaaga actcatggct gaccggtact gcggcgtgga
2461 actttgtggc gatttcacag tggatactgg gtgtaaaacc tgactatgac ggattgaaga
2521 ttgatccatg catacccaag gcatgggacg gatacaaagt taccagatat ttcagaggct
2581 caacttatga aatcactgtg aagaatccga accatgtatc aaaaggtgtg gctaaaatta
2641 ctgttgacgg caatgaaatc agcggaaata ttcttccggt gttcaatgac ggaaagactc
2701 acaaagttga agtaattatg ggataataaa gcaaggtatg acaaaaagaa gttatccggg
2761 tttttcggat aacttctttt ttaaaatagc ggcggttaaa actctattca atcctgcgtt
2821 ctttattgac aaaaatgata taccgcataa tggcaaatga ttgcatgttt actgcaattt
2881 acgc
//