LOCUS AY007258 11201 bp DNA linear BCT 25-JAN-2001
DEFINITION Pseudomonas fluorescens ATCC 13525 CztD (cztD) gene, partial cds;
CztC (cztC), CztB (cztB), CztA (cztA), CztS (cztS), CztR (cztR),
CztO (cztO), and ORF1 genes, complete cds; and methyl-accepting
chemoreceptor-like protein ORF2 gene, partial cds.
ACCESSION AY007258
VERSION AY007258.1
KEYWORDS .
SOURCE Pseudomonas fluorescens
ORGANISM Pseudomonas fluorescens
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 11201)
AUTHORS Feng,S.F. and Rossbach,S.
TITLE A locus involved in metal homeostasis in Pseudomonas fluorescens
encodes a proton/cation antiporter of the RND family and a
two-component system
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 11201)
AUTHORS Rossbach,S. and Feng,S.F.
TITLE Direct Submission
JOURNAL Submitted (18-AUG-2000) Biological Sciences, Western Michigan
University, 1903 West Michigan Avenue, Kalamazoo, MI 49008, USA
FEATURES Location/Qualifiers
source 1..11201
/organism="Pseudomonas fluorescens"
/mol_type="genomic DNA"
/strain="ATCC 13525"
/type_material="type strain of Pseudomonas fluorescens"
/db_xref="ATCC:13525"
/db_xref="taxon:294"
/note="metal tolerance, zinc, cadmium"
gene <1..258
/gene="cztD"
CDS <1..258
/gene="cztD"
/note="putative cation-diffusion-facilitator; similar to
CzcD of Ralstonia eutropha CH34"
/codon_start=1
/transl_table=11
/product="CztD"
/protein_id="AAG09626.1"
/translation="DIDQVEQAIRKVPGVKDIHDLHIWALTSGKNVLSTHLVADSTLG
TEQQILALVTEVLHEQFDISHVTLQVEGEGFHHDDHDDLHA"
gene 368..1588
/gene="cztC"
CDS 368..1588
/gene="cztC"
/note="putative outer membrane associated protein; similar
to CzcC of Ralstonia eutropha CH34"
/codon_start=1
/transl_table=11
/product="CztC"
/protein_id="AAG09627.1"
/translation="MAISVKTLLGATALWLAASAVQAQTLTLDRALQTAFANNPDLAA
AQWEIDIAQGGRQQAGLMPNPVASWDAEDTRRNTRTTTIKLSQTLELGGKRGARIDLA
TRAQDAAALTLEQRRNDLRADVIDSYYGALRAQERLDLAQRSVALAERGLVVANGRVR
AGKSSPVEATRAQVQVSQIRLEMDRAQIGLSDAYRRLAAITGSAAPDFQAVATQSQSA
PPLPAFTPLLARLEQTTQLRLAELHIQQNEASVGLEKAQRIPDLDVSIGSQYDAGVRE
RVNLVGVSMPIPLFNRNQGNVLAASRRADQARDLRNATELRLRTETRQALDLWQTANT
EVRAFNQQILPAAQSAVDSATRGFEMGKFNFLDVLDAQRTLIAARTQYLTATAQATDA
WVRIERIYGDLAQF"
gene 1607..2713
/gene="cztB"
CDS 1607..2713
/gene="cztB"
/note="putative membrane fusion protein; similar to CzcB
of Ralstonia eutropha CH34"
/codon_start=1
/transl_table=11
/product="CztB"
/protein_id="AAG09628.1"
/translation="MKHKHGVALAVALGLMLSGFANAEEEEGALELTEQQIQAAGIQL
AQAQPRAISSLLTLPGEVRFDEDRTSHIVPRAAGVVEAVQVNLGQSVKKGELLAVIAS
QQISDQRSELAASERRVELARTTFQRERQLWQDQISAEQDYLLARQALQEAEIALNNA
RQKMTALSGSAVLVGGNRYELRAPFAGMVVEKHLGVGEVVSETSNAFTLSDLSQVWVT
FGVFPKDLNKVRVGKPVKVSSTEMGTDVLGTVAYVGNLLGEQTRTATVRVNVPNPDAA
WRPGLFVTVQLATDTYQAKVTVPQTAIQTIEDKPSVFVRTPDGFITRHLELGVSENGY
VEVRQGLDAGAQVATAGSFILKSELGKGSAEHAH"
gene 2732..5887
/gene="cztA"
CDS 2732..5887
/gene="cztA"
/note="putative cation/proton antiporter; required for
tolerance to cadmium and zinc"
/codon_start=1
/transl_table=11
/product="CztA"
/protein_id="AAG09629.1"
/translation="MFERLIQFAIEQRIIVLLAMLLMAGVGIASYQKLPIDAVPDITN
VQVQINSAAAGFSPLETEQRITFPIETAMAGLPGLQQTRSLSRSGLSQVTVIFKDGTD
LFFARQLVNERLQVARGQLPTGIDTAMGPISTGLGEIFLWTVEAEDGALKEDGTPYTP
TDLRVIQDWIIKPQLRNVPGVAEINTIGGFAKEYQIAPDPKRLAAYNLTLSDLVTALE
RNNANVGAGYIERSGEQLLIRAPGQVASIDDIANIVITTSDGTPIRVRNVAQVDIGRE
LRTGAATENGREVVLGTVFMLIGENSRTVSQAVAKKLAEINRSLPAGVVAVTVYDRTN
LVEKAIATVKKNLFEGALLVVAVLFLFLGNIRAALITAMVIPLAMLFTFTGMFTNKVS
ANLMSLGALDFGIIVDGAVVIVENAIRRLAHAQQRHGRLLTRSERLHEVFAAAKEARR
ALIFGQLIIMVVYLPIFALTGVAGKMFHPMAFTVVIALLGAMILSVTFVPAAIALFVT
GKVKEEENLVMRTARRVYAPVLHWVMARRPWVFGLAVLTIVASGALASRMGSEFIPSL
SEGDFAQQALRVPGTSLTQSVQMQQQLEKTLLAQVPEIERVFARTGTAEIASDPMPPN
ISDSYVMLKPKAQWPDPNKSRETLIADIQRASAIVPGSAYELSQPIQLRFNELISGVR
SDVAVKVFGDDMAVLNKTAAEIAETLQKLEGASEVKVEQTSGLPVLTINIDREKAARF
GLNVGDVQDTIAVAVGGRQAGTLYEGDRRFDMVVRLSDALRTDIDGLSRLLIPVPALA
GSAAGQLGFIALSQVASLDLVLGPNQVSRENGKRLVIVSANVRGRDIGSFVEEAEAAI
NTQVKVPAGYWTTWGGQFEQLKEASERLRIVVPVALLLVFGLLFMMFNNLKDGLLVFT
GIPFALTGGIMALWLRDIPLSISAGVGFIALSGVAVLNGLVMIAFIRNLREEGRALSV
AVNEGALTRLRPVLMTALVASLGFIPMALATGTGAEVQRPLATVVIGGIISSTLLTLL
VLPALYQWAHRKEEETPGETR"
gene complement(5802..7238)
/gene="cztS"
CDS complement(5802..7238)
/gene="cztS"
/note="putative sensor kinase; required for tolerance to
cadmium and zinc"
/codon_start=1
/transl_table=11
/product="CztS"
/protein_id="AAG09630.1"
/translation="MKTGSLSMRLGLTVSVMGAGLVLLLATLAYLALTHELEKLARKG
LESKMEQIQHSVAQGMDLNTIRARPHSLMDLVIGHDNFYLTIVGTAPDESVLLSTGAK
PQTPLLTDFTPRETLGYLNWTDSYGNQVLSASSLMRLASGERVRVLLSLDRTDDQALL
SAYLRATVIALPMLLLLIGMGAWWLVQRGLAPLKQFSHVAAKVTTQDLTHRLSVDNLP
QELGELAQGFNVMLNRLDAGVQQLSQFSDDLAHELRAPLTNLMGKAQLTLSRQRPPDE
YKAVLESNTEELERLARIVSDMLFLAQVSHPAARASFAAVSLAAESERVMDLFALSAE
DKQLTLTLSGDAQVHGDRLMIQRALSNLLSNAIRHSPAGSHISLLVEAYGSTASVSVS
NPGPGIEARHLPHLFERFYRVDSSRARTEGGTGLGLAIVRSIMTLHQGQAEVRSLPGG
FTVFRLVFPLPCDAPTDKAPAAPARSAT"
gene complement(7235..7909)
/gene="cztR"
CDS complement(7235..7909)
/gene="cztR"
/note="putative response regulator; involved in cadmium
and zinc tolerance"
/codon_start=1
/transl_table=11
/product="CztR"
/protein_id="AAG09631.1"
/translation="MRILVVEDEQKTADYLQQGLSESGYVVDCASNGIDGLHLAGQHA
YELVILDVNLPGKDGWEVLEQLRRNGTQRVMMLTARGRLADKIKGLDMGADDYLVKPF
EFPELLARVRTLLRRSEHIPQPEVFSVQDLELDPRRHRAYRGSRRIDLTTKEFALLQV
LMRQTGEVLTRTQIISLVWDMNFDCDTNVVEVSISRLRAKVDDQSEVKLIHTIRGVGY
VLEARG"
gene complement(8254..9570)
/gene="cztO"
CDS complement(8254..9570)
/gene="cztO"
/note="putative outer membrane porin precursor; similar to
OprD3 of Pseudomonas aeruginosa"
/codon_start=1
/transl_table=11
/product="CztO"
/protein_id="AAG09632.1"
/translation="MNIAYYTFPFHCCRTALAAVAVEDKPEGFIEGSSVNVLARNFYF
NRDDRKGQSSPTGNGYSEAWAQGLIGKFESGFTQGTVGFGLDAFAMYGLKLDSGTGRS
GGKGSFGMLPVDSNNHPESGYSKFGGAAKVRVLDTVFKAGDVFPATPVVAAGDSRLLP
QSFRGVTAQNTSLEGLNLQGGRLSDMSQPSESGMNKGFATFYAGKVDAPWIGYVGGDY
TVNKHLSVSLYSSRLKDAWDQYYVGSAATYPLTDTVSLFGDVNYYKAVDEGKKLLGEF
DNNIWSARLGVRAGAHSVAVSHQRNNGDDDFDYLRQSDSIFLNNSIQYSDFNSPKERS
WMLRYDLDMQAFGIPGLSFMTRYGKGTGADYSHANAVYMRRDAAGNPLTDQRRWERDV
EARYVVQSGSLKDLSLRLRQATVRSSAFESDLEEVRLIAEYPLVAL"
CDS 9798..10130
/inference="non-experimental evidence, no additional
details recorded"
/note="hypothetical protein"
/codon_start=1
/transl_table=11
/product="ORF1"
/protein_id="AAG09633.1"
/translation="MNFYKIGLGVFTAILSFSAVAEGGGDRTFALMMERNEKAMAAYA
AKKGLPAPEVQAYRYGMTLDIANVVNVTPPIRSCNPVPSRMTYEDSSGKLNTLEYQVM
GICRNNGG"
CDS 10194..>11201
/inference="non-experimental evidence, no additional
details recorded"
/codon_start=1
/transl_table=11
/product="methyl-accepting chemoreceptor-like protein
ORF2"
/protein_id="AAG09634.1"
/translation="MQVRFISSNDHRCPMFNTRLKQELSALREELSSLQQVKESLESE
MLCLTLDAEGRVEWANANFLQELAYQATNILGRHIDDLVPAHVRQDEFQLRFKHALLR
GEHFAGTVRLMRGNGKEAWLRSILQPVRSSDGRIKHYSIYSSDLTRTIEASREHENLI
TALVRSTAVIEFDLGGHVLTANDRFLGGMGYSLAQIQGKHHRMFCEPEEYNSAEYQNF
WKRLNAGEFVAARFKRVDSHGRVVWLEATYNPVLDANDRLYKVVKFATVITDQVNQEQ
AVAEAANIAYSTSLQTDNSAQRGTTVVTQAVDVMRDLARHMQQAGDGIEALNAQSQVI
GT"
BASE COUNT 2204 a 3356 c 3486 g 2155 t
ORIGIN
1 gatatcgatc aggtcgagca ggccattcgc aaggtgcccg gggtcaagga catacacgac
61 ctgcatatct gggccctgac cagcggcaag aatgtgctga gcacgcactt ggtggcggac
121 tcgacgctcg gcaccgagca gcagatcctc gcgcttgtga cggaagtgct gcacgaacaa
181 ttcgatattt cccacgtcac cttgcaggtt gaaggcgaag ggtttcatca cgacgaccat
241 gacgacctgc acgcctgatc ctcgctaacg gatttgtaat gttctgccca ccgccctgat
301 aagtactgaa ggctacattg cccccgctgg gaaacctcag cgacttcatg ggcgtggaac
361 tttcgttatg gcaatttcgg ttaaaacact gttgggggcg actgcgttgt ggctggccgc
421 cagtgcggtc caggcgcaaa ccctcacgct tgatagagcc ctgcaaaccg cgtttgccaa
481 caaccctgac ctggccgccg cgcaatggga aatcgacatc gcccagggcg gtcgccaaca
541 ggcgggcttg atgcccaacc cggtggcctc ctgggatgct gaagacaccc gccgcaacac
601 gcgcaccacc acgatcaaac tcagccagac cctggagctg ggcggcaagc gcggtgcgcg
661 gattgacctg gcaacccgcg cccaggacgc tgccgcactg accctggagc agcgtcgcaa
721 cgacctgcgc gccgatgtga tcgacagcta ttatggcgcc ctacgggccc aggagcgcct
781 cgacctggcg caacgctctg tggccctggc cgagcgcggc ctggtggtcg ccaacggccg
841 tgtcagggcc ggcaagtcgt caccggtcga agcgacccgc gcgcaggtgc aggtctcgca
901 gattcgcctg gaaatggacc gtgcgcagat cggcctgagc gacgcctatc gccgcctcgc
961 agccattacc ggcagcgccg cgccggactt ccaggcggtt gcgacgcaaa gccaatctgc
1021 gccgccatta ccggccttca caccgttgct ggcgcgcctt gagcaaacca cgcagctgcg
1081 cctggctgag ctgcacattc agcaaaacga agccagcgta ggcctggaaa aagcccagcg
1141 gattcccgac ctcgacgtgt cgatcggcag ccagtacgac gccggcgtgc gcgagcgggt
1201 caacctggtg ggcgtgtcga tgccgatccc gctgttcaac cgcaaccagg gcaacgtgct
1261 cgccgccagc cgccgcgccg accaggcccg tgacttgcgc aacgccactg agttgcgcct
1321 gcgcaccgaa acccgccagg ccctggacct gtggcaaacc gcgaataccg aagtgcgcgc
1381 cttcaaccaa cagatcctgc ccgccgccca aagcgcggtg gacagcgcca cccggggttt
1441 cgagatgggc aagttcaact tcctcgacgt gctcgacgct cagcgcacct tgatcgccgc
1501 ccgcacccag tacctcacgg cgactgccca ggccaccgac gcctgggtac gcatcgaacg
1561 gatttacggc gacctcgccc agttctgatt ttttctggag ttttttatga agcataaaca
1621 cggtgtggcg ctggccgtcg ccctgggcct gatgctgtct gggtttgcaa acgccgaaga
1681 agaggagggc gcactcgaac tcactgagca gcagatccag gccgccggta ttcaactcgc
1741 ccaggcccag ccacgcgcca tcagcagcct gctgacactg ccgggagaag tgcgttttga
1801 tgaggatcgc acctcgcaca tcgtcccgcg cgcggccggt gtggtggagg cggtgcaggt
1861 caacctcggc cagtcggtta agaagggcga gctgttggcg gtgatcgcca gccagcaaat
1921 ttccgaccag cgcagtgagc tggcagccag tgagcggcgg gtcgaactgg cgcgcaccac
1981 cttccagcgc gagcgccaat tgtggcagga ccaaatctcc gccgagcagg attacctgct
2041 ggcgcgccag gccttgcagg aagctgagat cgccctgaac aacgcccgcc agaaaatgac
2101 cgccctcagt ggcagcgccg tcctggtcgg cggcaatcgc tatgaactgc gtgctccctt
2161 cgccggcatg gtggtggaaa agcacctggg agtcggcgag gtcgtcagcg aaaccagcaa
2221 cgcatttacc ctctcggacc tgtcgcaggt atgggtcacc ttcggtgtct ttcccaaaga
2281 cttgaacaag gtgcgcgtgg gtaaaccggt gaaggtcagc tctacggaaa tgggcaccga
2341 cgtgctcggc accgtggcct acgtcggcaa cttgctcggc gagcagacgc gtaccgccac
2401 cgtgcgcgtg aacgtgccca accccgacgc cgcctggcgc cccgggctgt ttgtcaccgt
2461 gcaattggcc actgacacct accaggccaa ggtcaccgtg cctcaaaccg cgattcagac
2521 catcgaggac aaaccctcgg tgttcgtgcg cacgccggac ggtttcatca cccgccacct
2581 tgagctgggc gtgagtgaaa acggctacgt cgaggtgcgc cagggcctcg acgccggggc
2641 acaggtggcg acggccggca gcttcatcct caagtccgaa ctgggtaaag gctcggccga
2701 gcacgcccat tgatcctgcg agtgattccc catgtttgag cgccttatcc aatttgccat
2761 cgagcagcgc atcatcgtgc tgctggccat gctgttgatg gccggtgtcg gcatcgccag
2821 ctatcagaaa ttgcccatcg acgcggtacc cgacatcacc aatgtgcagg tgcagatcaa
2881 ctcggcggcg gccgggtttt cgccgctgga aaccgaacag cgcattacct ttcccatcga
2941 aaccgccatg gccgggttgc cgggtctgca gcagacgcgg tcgctgtcgc gctccggttt
3001 gtcccaggtg acggtgattt tcaaagacgg taccgacctg ttcttcgccc gccaattggt
3061 caatgagcgc ctgcaggtcg cccgtggaca attgcctacc ggcatcgaca ccgccatggg
3121 gccgatttcc accggcctcg gggaaatttt cctgtggacc gtcgaggccg aggacggcgc
3181 actcaaagag gacggcacgc cctacacgcc gaccgacctg cgcgtgatcc aggactggat
3241 catcaagccg caactgcgca acgtgccggg tgtggccgag atcaacacca tcggcggctt
3301 tgccaaggaa tatcagattg ccccggaccc caagcgcctc gcggcctaca acctgacgtt
3361 gagtgatttg gtcacggccc ttgaacgcaa taacgccaac gtcggcgcgg gttacatcga
3421 gcgcagcggc gagcaactgc tgatccgcgc accggggcaa gtggcgtcga ttgatgacat
3481 cgccaatatc gtgatcacca cttccgatgg cacgccgatc cgcgtgcgca atgtggcgca
3541 ggtcgacatc ggccgtgagc tgcgcaccgg cgcggccacc gaaaacggcc gtgaagtggt
3601 gctgggcacg gtattcatgt tgatcggtga aaacagccgt accgtgtccc aggcggtggc
3661 gaaaaaactc gcagagatca accgttcgct accggcaggc gtggttgccg tgaccgtgta
3721 cgaccgcacc aacctggtgg aaaaagccat cgccaccgtg aagaaaaacc tcttcgaagg
3781 tgcgctgctg gtggttgcgg tgttgttcct gttcctgggc aacatccgcg cggcgttgat
3841 caccgcaatg gtgattcccc tggcgatgtt gttcaccttt accgggatgt ttaccaacaa
3901 agtcagcgcc aacctgatga gtcttggcgc actggacttc ggcattatcg tcgacggcgc
3961 ggtggtgatc gtcgagaacg ccatccgccg cctggcacat gcgcaacagc gccacggccg
4021 tttgttgacc cgcagcgagc ggttgcacga agtgttcgcg gcggccaaag aggcgcggcg
4081 ggcgctgatc ttcgggcaac tgatcatcat ggtggtttac ctgccgatct tcgccctcac
4141 gggtgtggcc gggaagatgt tccacccgat ggccttcacc gtggtgatcg cgctgctggg
4201 tgcgatgatt ctgtcggtga cgttcgtgcc ggccgcgatt gcgctgttcg tcaccggcaa
4261 ggtcaaggaa gaagaaaacc tggtgatgcg cacggcgcgg cgggtctatg cgccggtgct
4321 gcactgggta atggcacgcc gtccatgggt gtttggcttg gcggtgttga ccatcgtcgc
4381 ctctggggcg ctggccagcc gcatgggcag tgaattcatt cccagcctca gcgaaggcga
4441 cttcgcccaa caggccctgc gcgtgccggg caccagcctg acgcaatcgg tgcagatgca
4501 gcagcagctt gagaaaacat tgctggccca ggtgccggaa atcgagcggg tttttgcgcg
4561 caccggcacc gcagaaatcg cctcagaccc gatgccgccg aatatttccg acagctacgt
4621 aatgcttaaa ccgaaggcac agtggcccga tccaaacaaa tcccgcgaga cgctgattgc
4681 cgacatccag cgcgccagtg cgattgtgcc gggcagtgcg tatgaactgt cgcaaccgat
4741 ccagctgcgc ttcaacgagc tgatttccgg ggtgcgcagt gatgtggcag tgaaggtgtt
4801 tggtgacgac atggcggtgc tcaacaagac cgccgcggaa atcgccgaga ccctgcaaaa
4861 gctcgaaggc gcctcggaag tcaaggtcga gcagacctcc ggcttgccgg tgctgaccat
4921 caatatcgac cgcgagaagg ccgcgcgttt cggcctgaat gtgggcgatg tgcaggacac
4981 cattgcggtc gccgtgggcg ggcgtcaggc cggtacgttg tacgaaggtg atcgccgctt
5041 tgacatggtc gtgcgtttgt ccgacgcact acgcaccgat atcgacgggc tgtcacggct
5101 gctgattccg gtgccggcgc tggccggcag tgcggctggg cagttgggct ttattgcctt
5161 gtcccaggtc gccagcctgg acctggtgct cggtcccaac caggtcagcc gcgagaacgg
5221 caagcgcctg gtgatcgtca gcgccaacgt gcgcgggcgg gatatcggtt cgtttgtcga
5281 agaggccgag gcagccatca acacacaggt gaaagtcccc gccggttact ggaccacctg
5341 gggcggccag ttcgaacagt tgaaggaagc gtccgagcgt ttgcgcatcg tggtgccagt
5401 ggcgttgctg ttggtgttcg ggctgttgtt catgatgttc aacaacctca aggacggcct
5461 gttggtgttt accgggattc ctttcgcatt gacgggtggg atcatggcgc tgtggctgcg
5521 cgatattcca ttgtcgattt cggcgggggt gggctttatc gccttgtccg gtgtcgcggt
5581 gctcaacggg ctggtgatga ttgccttcat ccgcaacctg cgtgaggaag ggcgcgcgtt
5641 atcggtggcg gtcaacgagg gcgcgctgac ccgattgcgc ccggtattaa tgaccgcgct
5701 ggtggcgtcc ctggggttta tcccgatggc cctggccacc ggcaccggcg ccgaagtgca
5761 gcgcccgttg gcgacggtgg tgattggcgg gattatttct tctacgttgc tgaccttgct
5821 ggtgctgccg gcgctttatc agtgggcgca tcgcaaggaa gaggaaacac caggcgaaac
5881 acggtgaaac caccgggcag gctgcgcact tcagcctgcc cctggtgcag ggtcatgatc
5941 gagcgcacga tggccaggcc caggccggtg ccaccctcgg tgcgagcgcg gctgctgtcg
6001 acacggtaga agcgttcgaa caggtgcggc aggtggcgcg cttcgatgcc ggggccaggg
6061 ttgctgaccg acaccgacgc cgtgctcccg taggcttcca ccagcagcga aatatgcgag
6121 cccgccgggc tgtggcggat ggcattggac agcaggttcg acagggcgcg ctggatcatc
6181 aggcgatcgc cgtgcacctg agcatcgccg ctgagggtca gggtcagttg tttgtcttcg
6241 gcactgaggg cgaacaagtc catcacccgc tccgattcag ccgccagcga caccgccgcg
6301 aacgaagcgc gtgccgccgg gtggctgacc tgggccagaa acagcatgtc ggagacgata
6361 cgcgccaggc gctccagctc ttcggtgttg gattcgagca cggctttgta ttcgtcaggc
6421 gggcgctggc gcgacagcgt caactgggct ttgcccatca ggttggtcag gggcgcgcgc
6481 aattcgtggg caaggtcatc ggagaattgc gacagttgct gcaccccagc gtccaggcga
6541 ttgagcatca cattaaagcc ttgcgccaac tcgcccagct cttggggcag gttatcgacg
6601 gaaaggcgat gggtgaggtc ctgagtggtg actttggccg ccacatggct gaactgtttc
6661 aacggcgcca ggccgcgctg caccagccac caggcaccca tgccgatcag cagcagcagc
6721 atgggcaagg cgatcaccgt ggcgcgcaga taggcactga gcagcgcttg atcgtcggtg
6781 cggtccaggg acagcagcac gcgtacacgt tcgccgctgg ccaggcgcat caggctggag
6841 gcactgagca cctggttgcc ataactgtcg gtccagttga ggtaaccgag ggtttcccgt
6901 ggggtgaagt cagtgagcaa aggcgtctga ggcttggccc cggtgctcag cagcacgctt
6961 tcgtcggggg cggtgccgac gatggtcagg tagaagttgt catggccgat caccaggtcc
7021 atcagtgaat gcgggcgggc gcgaatggtg ttcaggtcca tgccctgggc cacgctgtgc
7081 tggatctgtt ccatcttgct ttccaggccc ttgcgcgcca gcttttccag ttcgtgggtc
7141 agggccaggt acgccagggt tgccagcaac agcaccaggc ccgcgcccat tacactgacc
7201 gtcagcccca ggcgcatcga caaactgccg gttttcatcc gcgcgcctcc agcacataac
7261 ccacgccgcg aatggtgtgg atcagcttga cctcgctctg gtcatcgacc ttggcccgca
7321 ggcggctgat ggagacttcc accacattgg tgtcgcagtc gaaattcatg tcccacacca
7381 gcgaaatgat ttgcgtgcgg gtcagcactt cgccggtttg gcgcatcaac acctgcagca
7441 gggcgaactc tttggtggtc aggtcgatcc gtcgggagcc gcgatacgcg cgatggcggc
7501 gcgggtccag ctccaggtcc tgcacgctga agacttccgg ttgcgggata tgctcactgc
7561 ggcgcagcag ggtgcggacc cgcgcgagca attcgggaaa ctcgaaaggc ttgaccaggt
7621 aatcatcggc gcccatgtcc aggcctttga tcttgtcggc cagccggcca cgcgcggtca
7681 gcatcatcac ccgttgcgtg ccgttgcggc gcaattgctc gagcacttcc cagccatcct
7741 tgcccggcag gttgacgtca agaatgacca actcataggc gtgctgcccg gccaggtgca
7801 ggccgtcgat gccattggac gcgcaatcga ccacatagcc actttcactc aggccttgct
7861 gcaaatagtc agcggttttt tgctcgtcct caaccacaag gatacgcata gaaaatttac
7921 cttttactga aggagaagag gtccatttcc ctatggaggc aggtgtcttg agagtaggga
7981 aattctgaag tctgaagtag cttcagggtc aactgcgacc attggtcgcg actcagccag
8041 cgggcaaaaa aaacgcccca gcggggcgtt aaaaattcat ctctttccaa aggagctaca
8101 caaaagcttc agagatgaat gtgctcggtg caacgaagtt gaagcgagta taggaagttc
8161 aacttaacga gaagctgaac gcaatgttac agttttgata gttatctatt tgtgagcaga
8221 tgttagttaa tggcgaacga atgtaaacgc aggtcaaagt gctactagcg ggtattcggc
8281 aatcaagcgc acttcctcca gatctgattc gaaagcgctg gagcgcacgg tggcctggcg
8341 taaccgcaat gacaaatcct tcaggctgcc actttgcacc acgtacctgg cctcgacgtc
8401 ccgctcccag cgacgctgat ccgtcagcgg attgcccgcc gcatcgcggc gcatgtacac
8461 cgcgttggca tggctgtaat cggcgccggt gcctttgccg tagcgggtca tgaacgacag
8521 gccgggaatg ccgaacgcct gcatgtcgag gtcgtagcgc agcatccacg agcgctcctt
8581 gggcgagttg aagtcgctgt actggatcga gttgttgagg aagatcgagt ccgattggcg
8641 caggtaatcg aagtcgtcgt cgccgttgtt gcgctggtgc gacacggcca cgctgtgggc
8701 gccggccctc accccgagcc tggcgctcca gatgttgtta tcgaattcgc ccagcagctt
8761 tttaccctcg tcgacggctt tgtaatagtt gacgtcgccg aacaaagaga cggtgtcggt
8821 cagcgggtaa gtggcggcac tgcccacgta atactgatcc caggcgtctt tcaggcggct
8881 gctgtagaga ctgacactga ggtgtttatt gacggtgtag tcgccgccga cgtaaccgat
8941 ccagggggca tcgaccttgc ccgcgtagaa cgtcgcaaag cctttgttca tgccactttc
9001 acttggctgg ctcatgtcgc tcaggcgccc gccctggagg ttcaggcctt caaggctggt
9061 gttttgcgcg gtgacaccgc gaaagctttg cggcaacagg cgtgagtcgc cggcggccac
9121 caccggcgtg gcggggaaca cgtcaccggc tttgaagacc gtgtcgagca cgcgcacttt
9181 cgcggcgccg ccgaatttgc tgtagccgct ttcggggtgg ttattgctgt ccaccggcag
9241 catgccgaac gaacccttgc cgccgctgcg gccggtgccg gagtcgagtt tcaggccgta
9301 catggcaaac gcatccaggc caaaccccac ggtgccttgg gtaaagccgg attcgaactt
9361 gccgatcaag ccctgcgccc aggcttcgga ataaccgttg ccggtggggc tggattggcc
9421 cttgcggtcg tcgcggttga agtaaaaatt gcgcgccagc acgttcacgc tgctgccttc
9481 gataaaacct tcaggcttgt cctccaccgc cacggctgcc agggcagtgc ggcagcaatg
9541 aaaagggaag gtgtagtaag caatattcat gtttcgctcc tcggtgttgg cagtgttcag
9601 cttcttatag gcagtgggcg ttcttattga tctggcccgg gctgatagtt gcaaacagcg
9661 gataacgact tcattgcgtg gacattacaa tcctggaaag tcacggctac ctaacaaata
9721 agtaatggtt tggtgaacat cgcgtcaggg ttgcttcatt acgcttgtca ctgaacttct
9781 tagacgagga acccgtaatg aacttttata aaattggcct gggtgtattc actgcaatcc
9841 tgtcgttcag tgcagtggcc gaaggcggtg gtgatcgcac ctttgccctg atgatggaac
9901 gcaatgagaa agccatggcg gcttacgccg caaaaaaggg gctgccggca cccgaggtac
9961 aggcctaccg ctatggtatg acgctggaca tcgccaacgt ggtcaacgtc accccaccga
10021 tccgttcgtg caatccggtg ccgtcgcgca tgacctatga agactccagc ggcaagctca
10081 atacccttga ataccaagtg atgggcatct gccggaataa tggtggttaa tcgcgccaga
10141 aaaacaggtg ggcgaagggc taaaaagcac ttcagaaaat cccggcagcg ccgatgcagg
10201 tcagattcat ctcctcaaat gatcatcggt gccccatgtt caacacccgt ttgaagcagg
10261 agctgtcagc tcttcgcgaa gagttgtcga gcttgcaaca ggtcaaggag agcctggaaa
10321 gtgaaatgct gtgcctgacg ctggatgccg aaggccgggt ggagtgggca aatgccaact
10381 tcctgcagga actggcgtac caggccacca acatcttggg gcgccacatt gatgatctgg
10441 tgccggcaca cgtgcgccag gacgaattcc agctgcgttt caagcatgcc ctgctgcgtg
10501 gcgaacactt cgctggcacc gtgcggctga tgcgcggcaa tggcaaggag gcgtggttgc
10561 gctcgatcct gcaacccgtg cgcagctcgg atgggcgtat caagcattat tcgatttact
10621 caagcgacct cacccgcacc atcgaggcct cgcgcgagca tgaaaacctg atcaccgcgc
10681 tggtgcgttc caccgcggtc atcgagttcg acctcggtgg gcatgtgctg acggccaatg
10741 accggttcct cggcggcatg ggttacagcc tggcgcagat tcagggcaag catcaccgga
10801 tgttctgcga accggaggaa tacaacagcg ccgagtacca gaacttctgg aaacgtttga
10861 atgccggtga gtttgtcgcg gcgcgtttca aacgcgtcga cagccatggg cgtgtggtct
10921 ggctggaagc tacctataac ccggtgttgg atgccaatga tcgcctgtac aaagtggtca
10981 agttcgccac ggtgattacc gaccaggtca accaggaaca ggccgtggcc gaggccgcca
11041 atattgccta cagcacctcg ctgcaaaccg acaacagtgc ccagcgtggc accacggtgg
11101 tgacgcaggc ggtggatgtg atgcgagatt tggccaggca catgcagcag gccggtgatg
11161 gcattgaggc gctcaacgcc cagtcgcaag tgatcggtac c
//