LOCUS AM284966 1578 bp DNA linear BCT 06-DEC-2006
DEFINITION Pseudomonas putida bfd gene for benzoylformate decarboxylase,
allele B.
ACCESSION AM284966
VERSION AM284966.1
KEYWORDS benzoylformate decarboxylase; bfd gene.
SOURCE Pseudomonas putida
ORGANISM Pseudomonas putida
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 1578)
AUTHORS Leggewie C.
JOURNAL Submitted (27-JUN-2006) to the INSDC. Leggewie C., Institute for
Molecular Enzyme Technol., Heinrich-Heine University Duesseldorf,
Stetternicher Forst, 52426 Juelich, GERMANY.
REFERENCE 2
AUTHORS Henning H., Leggewie C., Pohl M., Muller M., Eggert T., Jaeger K.E.
TITLE Identification of novel benzoylformate decarboxylases by growth
selection
JOURNAL Appl. Environ. Microbiol. 72(12), 7510-7517(2006).
PUBMED 17012586
FEATURES Location/Qualifiers
source 1..1578
/organism="Pseudomonas putida"
/strain="ATCC 12633"
/mol_type="genomic DNA"
/identified_by="Helge Henning"
/db_xref="taxon:303"
CDS 1..1578
/transl_table=11
/gene="bfd"
/allele="B"
/product="benzoylformate decarboxylase"
/EC_number="4.1.1.7"
/db_xref="GOA:Q17UB3"
/db_xref="InterPro:IPR000399"
/db_xref="InterPro:IPR011766"
/db_xref="InterPro:IPR012000"
/db_xref="InterPro:IPR012001"
/db_xref="InterPro:IPR029035"
/db_xref="InterPro:IPR029061"
/db_xref="UniProtKB/TrEMBL:Q17UB3"
/experiment="heterologous expression"
/protein_id="CAK95976.1"
/translation="MKTVHGATYDILRQHGLTTIFGNPGSNELPFLKGFPEDFRYILG
LHEGAVVGMADGYALASGQPTFVNLHAAAGTGNGMGALTNAWYSHSPLVITAGQQVRS
MIGVEAMLANVDAAQLPKPLVKWSHEPATAQDVPRALSQAIHTANLPPRGPVYVSIPY
DDWACEAPSGVEHLARRQVSSAGLPSPAQLQHLCERLAAARNPVLVLGPDVDGSAANG
LAVQLAEKLRMPAWVAPSASRCPFPTRHACFRGVLPAAIAGISHNLAGHDLILVVGAP
VFRYHQFAPGNYLPAGCELLHLTCDPGEAARAPMGDALVGDIALTLEAVLDGVPQSVR
QMPTALPAAEPVADDGGLLRPETVFDLLNALAPKDAIYVKESTSTVGAFWRRVEMREP
GSYFFPAAGGLGFGLPAAVGVQLASPGRQVIGVIGDGSANYGITALWTAAQYNIPVVF
IILKNGTYGALRWFADVLDVNDAPGLDVPGLDFCAIARGYGVQAVHAATGSAFAQALR
EALESDRPVLICAASSR"
BASE COUNT 235 a 544 c 523 g 276 t
ORIGIN
1 atgaaaactg ttcacggcgc cacttacgac attcttcgcc agcatggcct gaccaccatc
61 ttcggcaacc ccggctccaa cgagctgccg tttctcaagg gcttcccgga ggatttccgc
121 tacatcctcg gcctgcacga aggcgccgtg gtcggcatgg ccgatggcta cgcactggcc
181 agcggccagc ccaccttcgt caacctgcac gcggctgccg gtaccggcaa tggtatgggc
241 gccctcacca atgcctggta ctcgcacagc ccgctggtga ttaccgccgg ccagcaggtg
301 cgctcgatga tcggggtgga agcgatgctg gccaatgtcg atgccgcaca gctgcccaag
361 ccactggtca agtggagcca cgagcccgct accgcgcagg acgtgccgcg cgccctgagc
421 caggcgatcc acaccgccaa cttgccacca cgcgggccgg tgtacgtgtc gatcccctat
481 gacgactggg cgtgcgaagc gccaagcggt gtcgagcacc tggcacggcg ccaggtgagc
541 agtgccgggc tgccgtcgcc tgcgcagctg cagcacctgt gcgaacgcct ggcagcggcg
601 cgcaacccgg tgctggtgct gggcccggat gtcgatggca gcgccgccaa tggcctggct
661 gtgcagttgg ccgagaagct gcgcatgccg gcctgggtcg cgccttcggc gtcgcgctgc
721 ccgttcccca ctcggcatgc gtgcttccgc ggggttttgc cggcagccat tgccggcatc
781 agccacaatt tggccgggca cgacctgatc cttgtggtgg gggctccggt gttccgctac
841 caccagttcg ccccaggcaa ctacctgccg gccggctgcg agctgctgca cctgacctgt
901 gacccgggcg aagcggcgcg cgcgcccatg ggcgacgccc tggtcggcga cattgcgctt
961 accctcgagg ccgtgctgga cggcgtgccg cagagcgtgc ggcagatgcc caccgctttg
1021 cctgctgctg aaccggtggc ggatgacggt ggcctgttgc gccctgaaac agtgttcgac
1081 ctgctcaacg ccctggcgcc caaggacgcc atctacgtca aggagtccac ctctaccgtg
1141 ggtgcgttct ggcgccgcgt ggaaatgcgt gagccgggca gttatttctt ccccgcagcc
1201 ggtggcctgg gttttggcct gccagcagcc gtaggcgtgc agctggcatc gcctggccgg
1261 caggtgatcg gcgtgatcgg cgacggctcg gccaactatg gcatcaccgc gttgtggacc
1321 gccgcgcaat acaacatccc ggtggtgttc atcatcctca agaacggcac ctacggcgca
1381 ttgcgctggt tcgccgatgt gctcgacgtc aacgatgcgc ccgggctgga cgtgcctggc
1441 ctggacttct gcgccatcgc ccgtggctat ggcgtgcagg cggtgcatgc ggccactggc
1501 agcgccttcg cccaggcctt gcgtgaagcg ctggagagcg accggccagt gctgatctgc
1561 gcggcgagca gccgctga
//