LOCUS AK223570 1965 bp mRNA linear HUM 26-APR-2005
DEFINITION Homo sapiens mRNA for thioesterase, adipose associated isoform
BFIT2 variant, clone: FCC129A08.
ACCESSION AK223570
VERSION AK223570.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1965)
AUTHORS Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y., Tanaka,A. and
Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
FEATURES Location/Qualifiers
source 1..1965
/clone="FCC129A08"
/clone_lib="TYB_HRT"
/db_xref="H-InvDB:HIT000331350"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hst001001122"
/organism="Homo sapiens"
/tissue_type="Heart"
CDS <83..1867
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="thioesterase, adipose associated isoform BFIT2
variant"
/protein_id="BAD97290.1"
/translation="MIQNVGNHLRRGLASVFSNRTSRKSALRAGNDSAMADGEGYRNP
TEVQMSQLVLPCHTNQRGELSVGQLLKWIDTTACLSAERHAGCPCVTASMDDIYFEHT
ISVGQVVNIKAKVNRAFNSSMEVGIQVASEDLCSEKQWNVCKALATFVARREITKVKL
KQITPRTEEEKMEHSVAAERRRMRLVYADTIKDLLANCAIQDDLESRDCSRMVPAEKT
RVESVELVLPPHANHQGNTFGGQIMAWMENVATIAASRLCRAHPTLKAIEMFHFRGPS
QVGDRLVLKAIVNNAFKHSMEVGVCVEAYRQEAETHRRHINSAFMTFVVLDADDQPQL
LPWIRPQPGDGERWYREASARKKIRLDRKYIVSCKQTEVPLSVPWDPSNQVYLSYNNV
SSLKMLVAKDNWVLSSEISQVRLYTLEDDKFLSFHMEMVVHVDAAQAFLLLSDLRQRP
EWDKHYRSVELVQQVDEDDAIYHVTSPALGGHTKPQDFVILASRRKPCDNGDPYVIAL
RSVTLPTHRETPEYRRGETLCSGFCLWREGDQLTKVSYYNQATPGVLNYVTTNVAGLS
SEFYTTFKACEQFLLDNRNDLAPSLQTL"
BASE COUNT 414 a 601 c 580 g 370 t
ORIGIN
1 atttggagtc aggcctggct gttgctcagg tgaccagctt gtgtctctgg gagggcgctg
61 ctttccccgg ccacccggcg cgatgatcca gaatgtcgga aatcacctgc gacggggctt
121 ggcctctgtg ttctccaacc gcacatcccg gaagtcagcc ttacgtgcgg ggaacgacag
181 tgccatggca gacggcgagg gataccggaa ccccacggag gtgcagatga gccagctggt
241 gctgccctgc cacaccaacc aacgtggtga gctgagcgtc gggcagctgc tcaagtggat
301 tgacaccacg gcttgcctgt ccgcggagag gcacgctggc tgcccctgtg tcacagcttc
361 catggatgac atctattttg agcacaccat tagtgttgga caagtggtga atatcaaggc
421 caaggtgaac cgggccttca actccagcat ggaggtgggc atccaggtgg cctcggagga
481 cctgtgctct gagaagcagt ggaatgtgtg caaggccttg gccaccttcg tggcccgccg
541 agagatcacc aaggtgaagc tgaagcagat cacgccgcgg acagaagagg agaagatgga
601 gcacagtgtg gcggctgagc gccggcgcat gcgccttgtc tatgcagaca ccatcaagga
661 cctcctggcc aactgcgcca ttcaggacga tctggagagc agagactgta gccgcatggt
721 gccggctgag aagacccgtg tggagagtgt ggagctggtc ctgcctcccc acgccaatca
781 ccagggcaac acctttgggg gccagatcat ggcctggatg gagaatgtgg ccaccattgc
841 agccagccgg ctctgccgtg cccaccctac gctgaaggcc attgaaatgt tccacttccg
901 aggcccgtcc caggtcggcg accgtctggt gctcaaagcc atcgtgaaca atgccttcaa
961 acatagcatg gaggtgggcg tgtgcgtgga ggcctatcgc caggaggctg agacccaccg
1021 gcgccacatc aacagtgcct ttatgacctt tgtggtcctg gacgcagatg accagcccca
1081 gttgctgccc tggattcggc cccagcccgg cgatggtgag cggtggtacc gagaggccag
1141 tgccagaaag aagatccgcc tggacaggaa gtacatcgtg tcctgtaagc agacagaggt
1201 gcccctctcc gtcccctggg accctagcaa ccaggtgtac ctgagctaca ataacgtctc
1261 ctccttgaag atgcttgtgg ccaaggacaa ctgggtgctg tcctcggaga tcagtcaggt
1321 ccgcctgtac actctggagg atgacaagtt cctctccttc cacatggaga tggtggtgca
1381 tgtggatgca gcccaggcct tcctgctgct ctcggacctg cgtcagaggc cagagtggga
1441 caagcactac cggagcgtgg agctagtgca gcaggtagac gaggacgacg ccatctacca
1501 cgtcaccagc cctgccctcg gaggtcacac aaagccccag gacttcgtga tcctggcctc
1561 gaggcggaag ccttgtgaca atggggaccc ctatgtcatc gcgctgaggt cggtcacgct
1621 gcccacacac cgagagacgc cagagtacag acgcggagag accctctgct caggcttctg
1681 cctctggcgc gagggggacc agctgaccaa ggtatcctac tacaaccagg ccaccccagg
1741 tgttctcaac tatgtgacca ccaacgtggc cggcctctcc tctgagttct acaccacctt
1801 caaggcttgt gagcagtttc tcttggacaa ccggaatgat ctggccccca gcctccagac
1861 cctctagatg ccctcagtgg ccacatcatg cccactccca ctccatcctg tccccaagga
1921 ctcacataca gtgcctggag aaagccaaag acctttattt cttcc
//