LOCUS AJ601386 5321 bp DNA linear BCT 16-JAN-2004
DEFINITION Escherichia coli kanamycin-resistant Tn10-based transposon
cassette.
ACCESSION AJ601386
VERSION AJ601386.1
KEYWORDS IS10-right transposase; kanamycin phosphotransferase; kanR gene;
ORF1; transposon.
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1 to 5321)
AUTHORS Bourniquel A.A.
JOURNAL Submitted (23-OCT-2003) to the INSDC. Bourniquel A.A., Molecular
Microbiology, Biozentrum der Universitaet Basel, Klingelbergstrasse
50-70, CH-4056 Basel, SWITZERLAND.
REFERENCE 2
AUTHORS Bourniquel A.A., Hamburger F., Garcia P., Bickle T.A.
TITLE Cloning of the kanamycin-resistant Tn10 transposon cassette from
the pLOFKm construct [3] into a chloramphenicol-resistant vector,
pPG1, for use in transposon mutagenesis studies.
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Herrero M., de Lorenzo V., Timmis K.N.
TITLE Transposon vectors containing non-antibiotic resistance selection
markers for cloning and stable chromosomal insertion of foreign
genes in gram-negative bacteria
JOURNAL J. Bacteriol. 172(11), 6557-6567(1990).
PUBMED 2172216
FEATURES Location/Qualifiers
source 1..5321
/organism="Escherichia coli"
/mol_type="other DNA"
/note="mini-Tn10 based KanR transposon cassette"
/db_xref="taxon:562"
CDS 314..1522
/transl_table=11
/product="IS10-right transposase"
/function="involved in transposition"
/note="ORF1"
/db_xref="GOA:Q70BL4"
/db_xref="InterPro:IPR002559"
/db_xref="InterPro:IPR012337"
/db_xref="UniProtKB/TrEMBL:Q70BL4"
/experiment="experimental evidence, no additional details
recorded"
/protein_id="CAE53091.1"
/translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIR
TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNL
FTHGYALGKL"
mobile_element 2520..2562
/mobile_element_type="insertion sequence:IS10 R/out"
/rpt_type=INVERTED
repeat_region 2563..2696
/rpt_type=INVERTED
/note="133-bp repeat extending downstream from the IS10
inverted repeat"
repeat_region 2708..2973
/rpt_type=INVERTED
/note="265-bp repeat flanking the extended IS10 inverted
repeat"
CDS complement(3034..3849)
/transl_table=11
/gene="kanR"
/product="kanamycin phosphotransferase"
/function="kanamycin resistance protein"
/db_xref="GOA:Q79DX9"
/db_xref="InterPro:IPR002575"
/db_xref="InterPro:IPR011009"
/db_xref="InterPro:IPR024165"
/db_xref="UniProtKB/TrEMBL:Q79DX9"
/experiment="experimental evidence, no additional details
recorded"
/protein_id="CAE53092.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
repeat_region 3954..4219
/rpt_type=INVERTED
/note="265-bp repeat flanking the extended IS10 inverted
repeat"
repeat_region 4244..4377
/rpt_type=INVERTED
/note="133-bp repeat extending upstream from the IS10
inverted repeat"
mobile_element 4378..4420
/mobile_element_type="insertion sequence:IS10 R/out"
/rpt_type=INVERTED
BASE COUNT 1626 a 1194 c 1064 g 1437 t
ORIGIN
1 gaattctcat gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca
61 ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt
121 cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg
181 gcaaatattc tgaaatgagc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt
241 gtgagcggat aacaatttca cacaggaaac aggatcaaat ggtttcgcga aaaatcaata
301 atcagacaac aagatgtgcg aactcgatat tttacacgac tctctttacc aattctgccc
361 cgaattacac ttaaaacgac tcaacagctt aacgttggct tgccacgcat tacttgactg
421 taaaactctc actcttaccg aacttggccg taacctgcca accaaagcga gaacaaaaca
481 taacatcaaa cgaatcgacc gattgttagg taatcgtcac ctccacaaag agcgactcgc
541 tgtataccgt tggcatgcta gctttatctg ttcgggcaat acgatgccca ttgtacttgt
601 tgactggtct gatattcgtg agcaaaaacg acttatggta ttgcgagctt cagtcgcact
661 acacggtcgt tctgttactc tttatgagaa agcgttcccg ctttcagagc aatgttcaaa
721 gaaagctcat gaccaatttc tagccgacct tgcgagcatt ctaccgagta acaccacacc
781 gctcattgtc agtgatgctg gctttaaagt gccatggtat aaatccgttg agaagctggg
841 ttggtactgg ttaagtcgag taagaggaaa agtacaatat gcagacctag gagcggaaaa
901 ctggaaacct atcagcaact tacatgatat gtcatctagt cactcaaaga ctttaggcta
961 taagaggctg actaaaagca atccaatctc atgccaaatt ctattgtata aatctcgctc
1021 taaaggccga aaaaatcagc gctcgacacg gactcattgt caccacccgt cacctaaaat
1081 ctactcagcg tcggcaaagg agccatgggt tctagcaact aacttacctg ttgaaattcg
1141 aacacccaaa caacttgtta atatctattc gaagcgaatg cagattgaag aaaccttccg
1201 agacttgaaa agtcctgcct acggactagg cctacgccat agccgaacga gcagctcaga
1261 gcgttttgat atcatgctgc taatcgccct gatgcttcaa ctaacatgtt ggcttgcggg
1321 cgttcatgct cagaaacaag gttgggacaa gcacttccag gctaacacag tcagaaatcg
1381 aaacgtactc tcaacagttc gcttaggcat ggaagttttg cggcattctg gctacacaat
1441 aacaagggaa gacttactcg tggctgcaac cctactagct caaaatttat tcacacatgg
1501 ttacgctttg gggaaattat gaggggatct ctcagtgcat tgcctccaat tcccataatt
1561 tattacgccg ataataactt ggtgtaacct taaaaatgta cttaaatcga cgtgtaaaag
1621 attgttggga atcaaattga tattttaatg cgatctcaag gatagttttt ttcgtcaacc
1681 tcaactcaac agccgctttc gtcaaacgac gagcacgaat atagctagcc agtgtgaccc
1741 ctgttacttt ttgaacagcc gctgaaaata ccacttggta taacccgctt tattcgccac
1801 atcatcaagc agtaaagact gatctaaatt atgttcaatc cataatagaa catctttgat
1861 aaccgttgta tgaaactgct tatcatcata tcttaattgg atgttattag ctttattttg
1921 atagcgagaa tgctgttcaa tatacataaa ataacctaaa tgttcttaag attgtcacga
1981 ccacatcatc atgataccat aaacatactg acggtatgtt attttaaatc tatcatggaa
2041 aataaaaatc atcaacaaga aaattttaag agtacctatc aatcactggt taactcagca
2101 cgaatattgt ttgttgaaaa aggctatcaa gctgtttcaa tagatgagat cgatctcggg
2161 aaaagcgttg gtgaccaaag gtgcctttta tcatcacttt aaaaataaaa aacaattact
2221 cagtgcctgt tataagcagc aattaattat gattgatgcc tacatcacaa caaaaactga
2281 tttaacaaat ggttggtctg ccttagaaag tatatttgaa cattatcttg attatattat
2341 tgataataat aaaaacctta tccctatcca agaagtgatg cctatcattg gttggaatga
2401 acttgaaaaa attagccttg aatacattac tggtaaggta aacgccattg tcagcaaatt
2461 gatccaagag aaccaactta aagctttcct gacggaatgt taattctcgt tgaccctgag
2521 cactgatgaa tcccctaatg attttggtaa aaatcattaa gttaaggtgg atacacatct
2581 tgtcatatga tcccggatcc ggctgtaatc cgggcagcgc aacggaacat tcatcagtgt
2641 aaaaatggaa tcaataaagc cctgcgcagc gcgcagggtc agcctgaata cgcgtgcggc
2701 cgccgcgttt aatgaccagc acagtcgtga tggcaaggtc agaatagcgc tgaggtctgc
2761 ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga
2821 aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga
2881 acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca
2941 actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct
3001 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
3061 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
3121 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
3181 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
3241 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
3301 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
3361 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
3421 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
3481 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
3541 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
3601 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
3661 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
3721 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
3781 taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat
3841 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
3901 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg
3961 ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc
4021 agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc
4081 tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta
4141 tgagtcagca acaccttctt cacgaggcag acctcagcgc tattctgacc ttgccatcac
4201 gactgtgctg gtcattaaac gcggcggccg cggcctaggc ggccacgcgt attcaggctg
4261 accctgcgcg ctgcgcaggg ctttattgat tccattttta cactgatgaa tgttccgttg
4321 cgctgcccgg attacagccg gatccgggat catatgacaa gatgtgtatc caccttaact
4381 taatgatttt taccaaaatc attaggggat tcatcagtgc tcagggtcaa cgagaattaa
4441 cattccgtca ggaaagctta tgatgatgat gtgcttaaaa acttactcaa tggctggttt
4501 atgcatatcg caatacatgc gaaaaaccta aaagagcttg ccgataaaaa aggccaattt
4561 attgctattt accgcggctt tttattgagc ttgaaagata aataaaatag ataggtttta
4621 tttgaagcta aatcttcttt atcgtaaaaa atgccctctt gggttatcaa gagggtcatt
4681 atatttcgcg gaataacatc atttggtgac gaaataacta agcacttgtc tcctgtttac
4741 tcccctgagc ttgaggggtt aacatgaagg tcatcgatag caggataata atacagtaaa
4801 acgctaaacc aataatccaa atccagccat cccaaattgg tagtgaatga ttataaataa
4861 cagcaaacag taatgggcca ataacaccgg ttgcattggt aaggctcacc aataatccct
4921 gtaaagcacc ttgctgatga ctctttgttt ggatagacat cactccctgt aatgcaggta
4981 aagcgatccc accaccagcc aataaaatta aaacagggaa aactaaccaa ccttcagata
5041 taaacgctaa aaaggcaaat gcactactat ctgcaataaa tccgagcagt actgccgttt
5101 tttcgcccca tttagtggct attcttcctg ccacaaaggc ttggaatact gagtgtaaaa
5161 gaccaagacc cgctaatgaa aagccaacca tcatgctatt ccatccaaaa cgattttcgg
5221 taaatagcac ccacaccgtt gcgggaattt ggcctatcaa ttgcgctgaa aaataaataa
5281 tcaacaaaat gggcatcgtt ttaaataaag tgatgtatac c
//