LOCUS AJ010261 2168 bp DNA linear BCT 29-JUL-2008
DEFINITION Pseudomonas putida pepA gene.
ACCESSION AJ010261
VERSION AJ010261.1
KEYWORDS leucine aminopeptidase; pepA gene.
SOURCE Pseudomonas putida
ORGANISM Pseudomonas putida
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 2168)
AUTHORS Sonke T.
JOURNAL Submitted (21-AUG-1998) to the INSDC. Sonke T., Fine Chemicals, DSM
Research B.V., PO Box 18, 6160 MD Geleen, THE NETHERLANDS.
REFERENCE 2
AUTHORS Sonke T., Kaptein B., Boesten W.H.J., Broxterman Q.B., Kamphuis J.,
Formaggio F., Toniolo C., Rutjes F.P.J.T., Schoemaker H.E.
TITLE New developments in the enzymatic preparation and use of
enantiopure alpha-hydrogen and alpha,alpha-disubstituted
alpha-amino acids
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Sonke T., Kaptein B., Boesten W.H.J., Broxterman Q.B.,
Schoemaker H.E., Kamphuis J., Formaggio F., Toniolo C.,
Rutjes F.P.J.T.
TITLE Aminoamidase-catalyzed preparation and further transformations of
enantiopure alpha-hydrogen- and
alpha,alpha-disubstitutedalpha-amino acids
JOURNAL (in) Patel R.N. (Eds.); Stereoselective biocatalysis:23-28; Marcel
Dekker (2000)
FEATURES Location/Qualifiers
source 1..2168
/organism="Pseudomonas putida"
/strain="ATCC 12633"
/mol_type="genomic DNA"
/db_xref="taxon:303"
CDS 612..2105
/transl_table=11
/gene="pepA"
/standard_name="leucyl aminopeptidase"
/product="leucine aminopeptidase"
/EC_number="3.4.11.1"
/db_xref="GOA:O86436"
/db_xref="InterPro:IPR000819"
/db_xref="InterPro:IPR008283"
/db_xref="InterPro:IPR011356"
/db_xref="InterPro:IPR023042"
/db_xref="PDB:3H8E"
/db_xref="PDB:3H8F"
/db_xref="PDB:3H8G"
/db_xref="UniProtKB/Swiss-Prot:O86436"
/experiment="experimental evidence, no additional details
recorded"
/protein_id="CAA09054.1"
/translation="MELVVKSVAAASVKTATLVIPVGENRKLGAVAKAVDLASEGAIS
AVLKRGDLAGKPGQTLLLQNLQGLKAERVLLVGSGKDEALGDRTWRKLVASVAGVLKG
LNGADAVLALDDVAVNNRDAHYGKYRLLAETLLDGEYVFDRFKSQKVEPRALKKVTLL
ADKAGQAEVERAVKHASAIATGMAFTRDLGNLPPNLCHPSFLAEQAKELGKAHKALKV
EVLDEKKIKDLGMGAFYAVGQGSDQPPRLIVLNYQGGKKADKPFVLVGKGITFDTGGI
SLKPGAGMDEMKYDMCGAASVFGTLRAVLELQLPVNLVCLLACAENMPSGGATRPGDI
VTTMSGQTVEILNTDAEGRLVLCDTLTYAERFKPQAVIDIATLTGACIVALGSHTTGL
MGNNDDLVGQLLDAGKRADDRAWQLPLFDEYQEQLDSPFADMGNIGGPKAGTITAGCF
LSRFAKAYNWAHMDIAGTAWISGGKDKGATGRPVPLLTQYLLDRAGA"
BASE COUNT 453 a 722 c 675 g 318 t
ORIGIN
1 ccatggtcag ccccaacagg cgctgctggc tcataccggt tgcggacagc acggtcatct
61 cgctttccag gtacagccgc ccgtacgcca ggagaatgcc gaggaacagc cccagaggca
121 ggatcagttg caggaaaccc ggcagccgga agcccatgat caggaacagc acgcccgggt
181 cgagcacgcc ctgggccgcc tgggccagat acttgatgaa gcgcccgctc atgatgatca
241 ccagcagcac agcgctgacg gcgctcaagg tcaccaggac ctcgcgggag agataacgaa
301 agacgatcaa accagacact cctgggttgt cagggcggac tgccatgcaa tggcgccaaa
361 caatgactta gtacagccaa gaccaatgcc ggttcgccaa aggcgaacct ccatgtaaag
421 tgcgcgcatt atcctgtgat tgaccgcgcc tgtcacttgg ccgcgcatga aggcgcataa
481 cggggttgtc agcacgcttg ccgcaggctc aaactggcag cttttccgct ggcgcgcgac
541 tccgccgcca ggcccgtcca gagcgctgga acacacagcg ccattcgtat cgatcattcg
601 gggaccctga catggaactg gttgtaaaaa gcgtagctgc tgcatccgta aaaaccgcca
661 ccctggtcat tccggtaggt gaaaaccgca agctcggcgc tgttgccaag gctgtcgacc
721 tggccagcga aggtgccatc agcgctgtgc tcaagcgcgg cgacctggct ggcaagccag
781 gccagaccct gctgctgcag aacctgcagg gcctgaaagc cgagcgcgtg ctgctggtag
841 gcagcggcaa ggacgaagcc ctgggcgacc gcacctggcg caaactggtc gccagcgtgg
901 ccggcgtgct caagggcctg aatggcgccg atgcagtgct ggccctggac gatgtcgcgg
961 tcaacaaccg cgatgcacac tacggcaaat accgcctgct ggccgaaacc ctgctggacg
1021 gcgagtacgt gttcgaccgc ttcaagagcc agaaggtcga gccacgcgcc ctgaaaaaag
1081 tcaccctgct ggccgacaag gccggccagg ccgaggtcga gcgcgccgtc aagcacgcca
1141 gcgccattgc caccggcatg gccttcaccc gcgacctggg caacctgccg cctaacctgt
1201 gccacccaag cttcctcgcc gaacaggcca aggagctggg caaggcgcac aaggcgctga
1261 aggtcgaggt gctggacgag aagaagatca aggacctggg catgggcgcg ttctacgccg
1321 tgggccaggg cagcgaccag ccaccccgcc tgatcgtgct caactaccag ggcggcaaga
1381 aggcagacaa gccgttcgtg ctggtgggca agggcatcac cttcgatacc ggcggcatca
1441 gcctcaagcc aggcgccggc atggacgaaa tgaagtacga catgtgcggt gctgccagcg
1501 tgttcggcac cctgcgcgcg gtgctcgaac tgcaactgcc ggtcaacctg gtgtgcctgc
1561 tggcctgtgc cgagaacatg ccaagcggcg gtgccacccg cccgggtgac atcgttacca
1621 ccatgagcgg ccagaccgtc gaaatcctca acaccgacgc cgaaggccgc ctggtgctgt
1681 gcgacaccct gacctacgcc gagcgcttca agccgcaggc ggtgatcgac atcgccaccc
1741 tgaccggcgc ctgcatcgtc gccctgggca gccacaccac cggcctgatg ggcaacaacg
1801 acgacctggt tggccagttg ctcgacgctg gcaaacgcgc cgacgaccgt gcctggcagt
1861 tgccgctgtt cgatgaatac caggagcagc tggacagccc gttcgccgac atgggcaaca
1921 tcggtgggcc gaaggccggc accatcaccg caggctgctt cctgtcgcgc ttcgccaagg
1981 cctacaactg ggcgcacatg gacatcgccg gtaccgcatg gatcagtggt ggcaaggaca
2041 aaggcgccac aggccgcccg gtgcccctgc tgacccagta cctgctggac cgcgctggcg
2101 cctgacgccg tgagccggtg gtgccttgcg cactccggcc ggcgattacc accatgagca
2161 aagtcgac
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