LOCUS AF223641 3200 bp DNA circular SYN 24-MAY-2000
DEFINITION Cloning vector pUC21, complete sequence.
ACCESSION AF223641
VERSION AF223641.1
KEYWORDS .
SOURCE Cloning vector pUC21
ORGANISM Cloning vector pUC21
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3200)
AUTHORS Vieira,J. and Messing,J.
TITLE New pUC-derived cloning vectors with different selectable markers
and DNA replication origins
JOURNAL Gene 100, 189-194 (1991)
PUBMED 1905257
REFERENCE 2 (bases 1 to 3200)
AUTHORS Vieira,J. and Messing,J.
TITLE Direct Submission
JOURNAL Submitted (12-JAN-2000) Waksman Institute, Rutgers University, 190
Frelinghuysen Road, Piscataway, NJ 08854, USA
FEATURES Location/Qualifiers
source 1..3200
/organism="Cloning vector pUC21"
/mol_type="genomic DNA"
/db_xref="taxon:119972"
/lab_host="Escherichia coli"
gene 1400..2260
/gene="bla"
CDS 1400..2260
/gene="bla"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAF71714.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 772 a 825 c 790 g 813 t
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc gcggctcgta tgttgtgtgg
181 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccactag
241 tccgaggcct cgagatctat cgatgcatgc catggtaccc gggagctcga attcgaagct
301 tctgcagacg cgtcgacgtc atatggatcc gatatcgccg tggcggccgc tctagaacta
361 gtggatcgat ccccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
421 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
481 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
541 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
601 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
661 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
721 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
781 tctaaatcgg ggtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
841 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
901 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
961 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
1021 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
1081 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
1141 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
1201 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
1261 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
1321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
1381 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
1441 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
1501 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
1561 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
1621 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
1681 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
1741 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
1801 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
1861 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
1921 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
1981 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
2041 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
2101 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
2161 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
2221 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
2281 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
2341 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
2401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
2461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
2521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
2581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
2641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
2701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
2761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
2821 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
2881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
2941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
3001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
3061 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
3121 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
3181 agtgagcgag gaagcggaag
//