LOCUS AF173987 3339 bp DNA linear BCT 12-DEC-1999
DEFINITION Rhodothermus marinus glucosamine-6-phosphate deaminase homolog
gene, partial cds; mannosylglycerate synthase (mgs) gene, complete
cds; DNA ligase gene, partial cds; and unknown genes.
ACCESSION AF173987
VERSION AF173987.1
KEYWORDS .
SOURCE Rhodothermus marinus DSM 4252
ORGANISM Rhodothermus marinus DSM 4252
Bacteria; Rhodothermota; Rhodothermia; Rhodothermales;
Rhodothermaceae; Rhodothermus.
REFERENCE 1 (bases 1 to 3339)
AUTHORS Martins,L.O., Empadinhas,N., Marugg,J.D., Miguel,C., Ferreira,C.,
da Costa,M.S. and Santos,H.
TITLE Biosynthesis of mannosylglycerate in the thermophilic bacterium
Rhodothermus marinus. Biochemical and genetic characterization of a
mannosylglycerate synthase
JOURNAL J. Biol. Chem. 274 (50), 35407-35414 (1999)
PUBMED 10585410
REFERENCE 2 (bases 1 to 3339)
AUTHORS Marugg,J.D., Miguel,C. and da Costa,M.S.
TITLE Direct Submission
JOURNAL Submitted (30-JUL-1999) Departamento de Bioquimica, Universidade de
Coimbra, Praca Marques de Pombal, Coimbra 3001-401, Portugal
FEATURES Location/Qualifiers
source 1..3339
/organism="Rhodothermus marinus DSM 4252"
/mol_type="genomic DNA"
/strain="DSM 4252"
/type_material="type strain of Rhodothermus marinus"
/db_xref="taxon:518766"
CDS <1..660
/note="orfU"
/codon_start=1
/transl_table=11
/product="unknown"
/protein_id="AAF16903.1"
/translation="DLLLVPVAVRLDVVDHPVGKVHVRGIDVDMLEEVFPHEVVIALR
VGGRQSHVFVEVEGDDFGEVELTAAVAFDEAAVEAQRGASGGKAQHAVGFATDERGDE
LVGAFAQRLVIREDLDQHGLTSRLADGRSINLRGKARKGKRRRYWKRSQNAAMTRGWA
ITVVPGCANHGLSTHCAWHRCLGREVDWLVAQNGQLRGVFLKGWPVSFPEQNAGVAST
S"
CDS complement(<1..360)
/note="orfR"
/codon_start=1
/transl_table=11
/product="glucosamine-6-phosphate deaminase homolog"
/protein_id="AAF16904.1"
/translation="MLVEIFPDYETLSERAYELVATLIRRKPNCVLGFATGSTPLGLY
RRLVEGYRRGELDFSKVVTFNLDEYVGLPPSHPQSYHHFMWENLFQHININPSNVHLP
NGMVDDIEAHCDWYEEQI"
gene 667..1860
/gene="mgs"
CDS 667..1860
/gene="mgs"
/codon_start=1
/transl_table=11
/product="mannosylglycerate synthase"
/protein_id="AAF16905.1"
/translation="MSLVVFPFKHEHPEVLLHNVRVAAAHPRVHEVLCIGYERDQTYE
AVERAAPEISRATGTPVSVRLQERLGTLRPGKGDGMNTALRYFLEETQWERIHFYDAD
ITSFGPDWITKAEEAADFGYGLVRHYFPRASTDAMITWMITRTGFALLWPHTELSWIE
QPLGGELLMRREVAAMLYEDERVRRRSDWGIDTLYTFVTVQQGVSIYECYIPEGKAHR
LYGGLDDLRTMLVECFAAIQSLQHEVVGQPAIHRQEHPHRVPVHIAERVGYDVEATLH
RLMQHWTPRQVELLELFTTPVREGLRTCQRRPAFNFMDEMAWAATYHVLLEHFQPGDP
DWEELLFKLWTTRVLNYTMTVALRGYDYAQQYLYRMLGRYRYQAALENGRGHPVPPRA
ALSTA"
CDS 1931..2857
/note="orfD"
/codon_start=1
/transl_table=11
/product="unknown"
/protein_id="AAF16906.1"
/translation="MREQAVLENMRRLAHDLRRIREKQKRSLQEIHNETKIALELLQH
FERTALYDHERYNPVYLRSFARTYASAIGIDPAAVLDAVELAFEGRYRNQLAVRFLGE
EPLPEPEPETAPAEASTPEAAPERKPPQESETPLPLVTTPGGLERLERPARWKARVVG
VIVVVMAAAGIWYLLRPAPAPPPEPLPVDTATVPAAATDTTPSVETPPVQVPVLGDTI
TATVIAAFDKVDPIRVRIDRDLRRPYWIEQGDSMQFRFTERIILENQLDDIQLRLDGH
PYPTDRRDAEGRIVITREQLQAYLDSLARGVQ"
CDS 2878..>3339
/EC_number="6.5.1.2"
/note="lig"
/codon_start=1
/transl_table=11
/product="DNA ligase"
/protein_id="AAF16907.1"
/translation="METHTAPQTAEARLLEATHTLLQTVRQRDLEAIDRKEAEALAAR
LREVLNQHAYRYYVLDNPLIPDADYDLLMQALRKLEARFPELVTPDSPTQRVGGPPLG
RFEKVRHPEPLLSLNNAFGEEDVRVWYERCCRMLAERLGQPVQPAVTAELKI"
BASE COUNT 641 a 1051 c 1073 g 574 t
ORIGIN
1 gatctgctcc tcgtaccagt cgcagtgcgc ctcgatgtcg tcgaccatcc cgttgggaag
61 gtgcacgttc gagggattga tgttgatatg ttggaagagg ttttcccaca tgaagtggtg
121 atagctctga gggtgggagg gcggcagtcc cacgtattcg tcgaggttga aggtgacgac
181 tttggagaag tcgagctcac cgcggcggta gccttcgacg aggcggcggt agaggcccag
241 cggggtgctt ccggtggcaa agcccagcac gcagttgggt ttgcgacgga tgagcgtggc
301 gacgagctcg taggcgcgtt cgctcagcgt ctcgtaatcc gggaagatct cgaccagcat
361 ggtctgacaa gcaggttagc tgatggcagg tccatcaatc tcagaggaaa agcgaggaaa
421 ggcaagaggc gacgttactg gaaacgatcc caaaatgcag caatgacgcg cggctgggcg
481 atcacggtcg tgccaggatg cgctaatcac ggcctgtcga cacattgcgc ctggcaccgg
541 tgcttagggc gggaggtgga ttggcttgtt gcacaaaatg gacagctccg gggtgttttt
601 ctgaagggat ggcccgtatc tttccctgag caaaatgcgg gcgtagcgtc aaccagctga
661 atcgccatga gcctggtcgt ttttcccttc aagcacgaac accccgaggt gctgctgcac
721 aacgtgcgcg tggcggctgc tcatccccgg gtgcacgagg tgctctgcat cggatacgag
781 cgcgaccaga cctacgaggc cgtcgaacgg gccgcgcccg aaatctcgcg ggccacaggg
841 acgccggtgt cggtccggtt gcaggagcga ctgggcacgc tgcggcccgg caagggcgac
901 ggcatgaaca ccgcgctccg gtatttcctg gaagagacgc agtgggagcg catccacttc
961 tacgatgccg acatcaccag cttcgggccg gactggatca ccaaggccga agaggcggcc
1021 gatttcggct acgggctggt gcggcactac ttcccgcggg cctcaaccga cgcaatgatc
1081 acttggatga tcacgcgcac gggctttgcc ctgctctggc cgcataccga actgtcgtgg
1141 atcgagcagc cgctgggcgg cgagctgctc atgcggcgcg aagtggccgc catgctttac
1201 gaggatgaac gggtgcgacg gcggagcgac tggggcatcg acacactcta cacgttcgtt
1261 accgtgcagc agggcgtttc gatctacgag tgctacattc ctgagggcaa ggcgcatcgc
1321 ctctacggcg ggctcgacga cctgcgcacc atgctggtcg agtgttttgc cgccatccag
1381 agtctgcaac atgaggtcgt tggccagccg gccattcatc gccaggagca cccgcaccgc
1441 gtgccggtcc acattgccga acgcgtgggc tacgacgtgg aggccacgct gcatcgcctc
1501 atgcagcact ggacgccccg gcaggtggag ctgctggagc tttttaccac acccgtgcgc
1561 gaaggactgc gcacctgtca gcgtcgcccg gctttcaact tcatggacga gatggcctgg
1621 gcggccacct atcacgtgct gctcgagcac tttcagccgg gcgatccgga ctgggaggag
1681 ctgctcttca aactctggac gacccgcgtg ctcaactaca cgatgacggt cgcgctgcgg
1741 ggctacgact acgcgcagca gtatctctac cggatgctgg gacgctaccg ctatcaggcc
1801 gctctggaaa acggccgtgg tcatccggtc ccgccgcggg cggcactgtc gaccgcctga
1861 cgaaacacgg acgcaccggc cgggtgcaat actggaccaa tccagatgtt gctggcaaaa
1921 gctcctcacc atgcgtgaac aagcagtcct ggaaaatatg cgccggctgg cgcacgacct
1981 gcggcgcatc cgggaaaagc agaagcgctc gctgcaggag atccacaacg agaccaaaat
2041 cgcgctggag ctgctgcagc acttcgagcg tacggcgctc tacgatcacg agcgatacaa
2101 ccccgtttat ctgcgttcgt ttgcccggac gtatgcaagc gctatcggca tcgacccggc
2161 tgctgtgctg gatgcggtag aactggcctt cgaagggcgc tatcgcaacc agctggccgt
2221 tcggtttctg ggggaagaac cgctgcccga accggagccg gaaaccgcgc cggccgaagc
2281 gtcgacccct gaagccgcgc ctgagcgcaa gccgcctcaa gagtctgaaa caccgctacc
2341 cctggtgacc accccgggtg gtctggaacg gctggaacgc cctgcgcgct ggaaggcccg
2401 ggtcgtgggc gtcatcgtgg tggtgatggc ggccgcaggc atctggtatc tgctgcgacc
2461 ggctccggca ccacctccgg agccgctgcc cgtggatacg gccacggttc cggctgctgc
2521 gaccgatacg acaccttcgg ttgaaacgcc ccccgttcag gtgcccgtgc tgggcgatac
2581 gatcacggcc accgtcattg ccgccttcga caaggtcgat cccatccggg tccggatcga
2641 tcgcgacctg cgccgcccct actggatcga gcagggcgac tcgatgcaat ttcggtttac
2701 cgaacgcatc atccttgaaa accagctgga cgacatccag ctgcggctgg acggccatcc
2761 gtatcccacc gaccgccggg atgccgaggg ccgcattgtc atcacccggg agcagttgca
2821 ggcttacctg gattcgctgg cacgaggggt ccagtagcag caaaaccaga gcacaccatg
2881 gagacgcaca ccgcaccgca aaccgccgaa gcacgcctgc tggaggccac acacacgctg
2941 ttgcaaactg tccgacagcg cgacctggaa gccatcgacc gcaaggaggc cgaggcgctg
3001 gcggcccggc tccgggaagt gctcaaccaa cacgcctacc gctattacgt actggacaac
3061 ccgctcatcc cggacgcgga ctacgacctg ctcatgcagg cgcttcggaa gctcgaggcg
3121 cgtttcccgg agctggtcac gcccgactca cccacgcagc gcgtgggagg accgccgctg
3181 ggacgcttcg aaaaggtgcg ccatccggag ccgctccttt cgctcaacaa cgcttttggc
3241 gaggaagacg tgcgcgtctg gtacgagcgc tgttgccgga tgctggccga gcgactcggg
3301 cagccggtgc agccggccgt caccgccgag ctgaagatc
//