LOCUS AF170104 8654 bp DNA linear BCT 26-JUL-2016
DEFINITION Vibrio fischeri regulatory protein LuxR (luxR) gene, complete cds;
LuxICDABEG operon, complete sequence; and unknown gene.
ACCESSION AF170104
VERSION AF170104.1
KEYWORDS .
SOURCE Aliivibrio fischeri (Vibrio fischeri)
ORGANISM Aliivibrio fischeri
Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
Vibrionaceae; Aliivibrio.
REFERENCE 1 (bases 1 to 8654)
AUTHORS Knight,T. and Papadakis,N.
TITLE Vibrio fischeri Lux operon SalI digest
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8654)
AUTHORS Knight,T. and Papadakis,N.
TITLE Direct Submission
JOURNAL Submitted (19-JUL-1999) Artificial Intelligence Laboratory, MIT,
545 Technology Square, Cambridge, MA 02139, USA
FEATURES Location/Qualifiers
source 1..8654
/organism="Aliivibrio fischeri"
/mol_type="genomic DNA"
/strain="MJ-1"
/db_xref="taxon:668"
/note="Fotodyne stab"
gene complement(216..1047)
/gene="luxR"
CDS complement(216..968)
/gene="luxR"
/codon_start=1
/transl_table=11
/product="regulatory protein LuxR"
/protein_id="AAD48473.1"
/translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLA
IIYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAV
NKKSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPL
IVPSLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHL
TNAQMKLNTTNRCQSISKAILTGAIDCPYFKN"
regulatory complement(974..981)
/regulatory_class="ribosome_binding_site"
/gene="luxR"
/note="putative"
repeat_region complement(1005..1015)
/gene="luxR"
/note="putative LuxR binding site"
/rpt_type=inverted
misc_feature complement(1011)
/gene="luxR"
/note="transcription start"
regulatory complement(1014..1019)
/regulatory_class="minus_10_signal"
/gene="luxR"
/note="putative sigma 70 binding site"
regulatory complement(1041..1047)
/regulatory_class="minus_35_signal"
/gene="luxR"
/note="putative sigma 70 binding site"
gene 1071..8273
/gene="LuxICDABEG"
misc_feature 1071..1077
/gene="LuxICDABEG"
/note="CAP/cAMP control binding site"
regulatory 1154..1160
/regulatory_class="minus_10_signal"
/gene="LuxICDABEG"
/note="putative sigma 70 binding site"
misc_feature 1166
/gene="LuxICDABEG"
/note="transcription start"
gene 1174..1768
/gene="luxI"
regulatory 1174..1181
/regulatory_class="ribosome_binding_site"
/gene="luxI"
/note="putative"
CDS 1187..1768
/gene="luxI"
/codon_start=1
/transl_table=11
/product="autoinducer synthesis protein LuxI"
/protein_id="AAD48474.1"
/translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLE
SDEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVEL
SRFAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPC
HRIGDKEIHVLGDTKSVVLSMPINEQFKKAVLN"
repeat_region 1201..1218
/gene="luxI"
/rpt_type=inverted
gene 1812..3261
/gene="luxC"
regulatory 1812..1815
/regulatory_class="ribosome_binding_site"
/gene="luxC"
/note="putative"
CDS 1822..3261
/gene="luxC"
/codon_start=1
/transl_table=11
/product="acyl-CoA reductase LuxC"
/protein_id="AAD48475.1"
/translation="MNKCIPMIINGMIQDFDNYAYKEVKLNNDNRVKLSVITESSVSK
TLNIKDRINLNLNQIVNFLYTVGQRWKSEEYNRRRTYIRELKTYLGYSDEMARLEANW
IAMLLCSKSALYDIVNYDLGSIHVLDEWLPRGDCYVKAQPKGVSVHLLAGNVPLSGVT
SILRAILTKNECIIKTSSSDPFTANALVSSFIDVNADHPITKSMSVMYWPHDEDMTLS
QRIMNHADVVIAWGGDEAIKWAVKYSPPHVDILKFGPKKSLSIIEAPKDIEAAAMGVA
HDICFYDQQACFSTQDVYYIGDNLPLFLNELEKQLDRYAKILPKGSNSFDEKAAFTLT
EKESLFAGYEVRKGDKQAWLIVVSPTNSFGNQPLSRSVYVHQVSDIKEIIPFVNKNRT
QTVSIYPWEASLKYRDKLARSGVERIVESGMNNIFRVGGAHDSLSPLQYLVRFVSHER
PFNYTTKDVAVEIEQTRYLEEDKFLVFVP"
gene 3265..4199
/gene="luxD"
regulatory 3265..3269
/regulatory_class="ribosome_binding_site"
/gene="luxD"
/note="putative"
CDS 3276..4199
/gene="luxD"
/note="ACT; myristoyl-ACP-specific thioesterase"
/codon_start=1
/transl_table=11
/product="acyl-transferase LuxD"
/protein_id="AAD48476.1"
/translation="MKDESAFFTIDHIIKLDNGQSIRVWETLPKKNVPEKKHTILIAS
GFARRMDHFAGLAEYLSTNGFHVIRYDSLHHVGLSSGCINEFTMSIGKNSLLTVVDWL
TDHGVERIGLIAASLSARIAYEVVNKIKLSFLITAVGVVNLRDTLEKALEYDYLQLPI
SELPEDLDFEGHNLGSEVFVTDCFKHNWDTLDSTLNSVKGLAIPFIAFTANDDSWVKQ
SEVIELIDSIESSNCKLYSLIGSSHDLGENLVVLRNFYQSVTKAALALDDGLLDLEID
IIEPRFEDVTSITVKERRLKNEIENELLELA"
gene 4217..5294
/gene="luxA"
regulatory 4217..4222
/regulatory_class="ribosome_binding_site"
/gene="luxA"
/note="putative"
CDS 4230..5294
/gene="luxA"
/note="luciferase alpha chain"
/codon_start=1
/transl_table=11
/product="alkanal monooxygenase alpha chain LuxA"
/protein_id="AAD48477.1"
/translation="MKFGNICFSYQPPGETHKQVMDRFVRLGIASEEVGFDTYWTLEH
HFTEFGLTGNLFVAAANLLGRTKTLNVGTMGVVIPTAHPVRQLEDVLLLDQMSKGRFN
FGTVRGLYHKDFRVFGVDMEESRAITQNFYQMIMESLQTGTISSDSDYIQFPKVDVYP
KVYSKNVPTCMTAESASTTEWLAIQGLPMVLSWIIGTNEKKAQMELYNEIATEYGHDI
SKIDHCMTYICSVDDDAQKAQDVCREFLKNWYDSYVNATNIFNDSNQTRGYDYHKGQW
RDFVLQGHTNTNRRVDYSNGINPVGTPEQCIEIIQRDIDATGITNITCGFEANGTEDE
IIASMRRFMTQVAPFLKEPK"
gene 5320..6313
/gene="luxB"
regulatory 5320..5325
/regulatory_class="ribosome_binding_site"
/gene="luxB"
/note="putative"
CDS 5333..6313
/gene="luxB"
/note="luciferase beta chain"
/codon_start=1
/transl_table=11
/product="alkanal monooxygenase beta chain LuxB"
/protein_id="AAD48478.1"
/translation="MKFGLFFLNFQKDGITSEETLDNMVKTVTLIDSTKYHFNTAFVN
EHHFSKNGIVGAPITAAGFLLGLTNKLHIGSLNQVITTHHPVRVAEEASLLDQMSEGR
FILGFSDCESDFEMEFFRRHISSRQQQFEACYEIINDALTTGYCHPQNDFYDFPKVSI
NPHCYSENGPKQYVSATSKEVVMWAAKKALPLTFKWEDNLETKERYAILYNKTAQQYG
IDISDVDHQLTVIANLNADRSTAQEEVREYLKDYITETYPQMDRDEKINCIIEENAVG
SHDDYYESTKLAVEKTGSKNILLSFESMSDIKDVKDIIDMLNQKIEMNLP"
gene 6376..7524
/gene="luxE"
regulatory 6376..6382
/regulatory_class="ribosome_binding_site"
/gene="luxE"
/note="putative"
CDS 6388..7524
/gene="luxE"
/note="long chain fatty acid luciferin component ligase"
/codon_start=1
/transl_table=11
/product="LuxE"
/protein_id="AAD48479.1"
/translation="MTVHTEYKRNQIIASSEIDDLIFMTKPQEWSFEEQKEIRDKLVR
EAFYFHYNRNEEYRNYCINQHVSDNLHTIDEIPVFPTSVFKYKKLHTVTAEDIENWYT
SSGTRGVKSHIARDRLSIERLLGSVNFGMKYVGDWFEHQMELINLGPDRFNTNNIWFK
YVMSLVELLYPTEFTVDNDKIDFEKTVKHLFRIKNSKKDICLIGPPFFVYLLCQYMKE
NNIEFKGGDRVHIITGGGWKSNQNDSLDRADFNQLLMDTFQLDKINQIRDTFNQVELN
TCFFEDEFQRKHVPPWVYARALDPETLKPVADGEIGLLSYMDASSTAYPAFIVTDDIG
IVKEIREPDPYPGVTVEIVRRLNTRAQKGCALSMANVIQKNIKD"
gene 7516..8238
/gene="luxG"
regulatory 7516..7520
/regulatory_class="ribosome_binding_site"
/gene="luxG"
/note="putative"
CDS 7528..8238
/gene="luxG"
/note="probable flavin reductase"
/codon_start=1
/transl_table=11
/product="LuxG"
/protein_id="AAD48480.1"
/translation="MIVDGRVSKIVLASIRNNIYKVFITVNSPIKFIAGQFVMVTING
KKCPFSIANCPTKNYEIELHIGSSNRDCSLDIIEYFVDALVEEVAIELDAPHGNAWLR
SESNNPLLLIAGGTGLSYINSILTNCLNRNIPQDIYLYWGVKDSSLLYEDEELLNLSL
NNKNFHYIPVIEDKSEEWIGRKGTVLDAVMEDFTDLTYFDIYVCGPFMMAKTAKEKLI
EEKKAKSEQMFADAFAYV"
regulatory 8251..8273
/regulatory_class="terminator"
/gene="LuxICDABEG"
/note="inverted repeat; bidirectional termination"
CDS complement(8279..>8654)
/note="ORF-V"
/codon_start=2
/transl_table=11
/product="unknown"
/protein_id="AAD48481.1"
/translation="STQVGAVITKHNRIVSVGFNGYPHGVSDSADTDEREIKYLKTLH
AEENAILFAKRDLEGCDIWVTHFPCPNCAAKIIQTGISKVYCPEQTEDFLSRWGEKIQ
VSQDMFSQAGVEVTWLPLDTLK"
BASE COUNT 2927 a 1268 c 1648 g 2811 t
ORIGIN
1 gtcgaccttc ctggttcaga gcctcatatc catattacga ccacttcaat gggcgatcaa
61 aaagtacatt acatgatttt ttgcccaaca gaaaaagcct ctcatttaga gcagcttatt
121 cgtcaagatt tcatggaaat gtatgagatc aattttccac aaaaaacaga gtaacaaaga
181 gaaaaaccta taggtataaa gctttactta cgtacttaat ttttaaagta tgggcaatca
241 attgctcctg ttaaaattgc tttagaaata ctttggcagc ggtttgttgt attgagtttc
301 atttgcgcat tggttaaatg gaaagtgaca gtacgctcac tgcagcctaa tatttttgaa
361 atatcccaag agctttttcc ttcgcatgcc cacgctaaac attctttttc tcttttggtt
421 aaatcgttgt ttgatttatt atttgctata tttatttttc gataattatc aactagagaa
481 ggaacaatta atggtatgtt catacacgca tgtaaaaata aactatctat atagttgtct
541 ttttctgaat gtgcaaaact aagcattccg aagccattgt tagccgtatg aatagggaaa
601 ctaaacccag tgataagacc tgatgttttc gcttctttaa ttacatttgg agatttttta
661 tttacagcat tgttttcaaa tatattccaa ttaattggtg aatgattgga gttagaataa
721 tctactatag gatcatattt tattaaatta gcgtcatcat aatattgcct ccatttttta
781 gggtaattat ctagaattga aatatcagat ttaaccatag aatgaggata aatgatcgcg
841 agtaaataat attcacaatg taccatttta gtcatatcag ataagcattg attaatatca
901 ttattgcttc tacaagcttt aattttatta attattctgt atgtgtcgtc ggcatttatg
961 tttttcatac ccatctcttt atccttacct attgtttgtc gcaagttttg cgtgttatat
1021 atcattaaaa cggtaatgga ttgacatttg attctaataa attggatttt tgtcacacta
1081 ttgtatcgct gggaatacaa ttacttaaca taagcacctg taggatcgta caggtttacg
1141 caagaaaatg gtttgttata gtcgaataaa cgcaagggag gttggtatga ctataatgat
1201 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg
1261 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc
1321 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt
1381 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc
1441 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt
1501 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact
1561 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac
1621 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga
1681 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga
1741 acagtttaaa aaagcagtct taaattaatg ttgttaaatc attaatttat tttaaatact
1801 aagtatatta tagggaaaat aatgaataaa tgtattccaa tgataattaa tggaatgatt
1861 caagattttg ataattatgc atataaagaa gttaaactaa ataatgataa tagagtaaaa
1921 ttatctgtca ttactgaaag ttcagtttca aaaacattaa atatcaaaga tagaattaat
1981 ctaaatttaa atcagattgt gaatttttta tataccgttg gtcaacgatg gaaaagtgaa
2041 gaatataatc ggcgacgaac ctatatccgt gagttaaaaa catatcttgg ttattctgat
2101 gaaatggcaa gattagaagc gaattggatt gcaatgttat tgtgctctaa aagtgctttg
2161 tatgacattg ttaattatga tttgggctct atacacgtat tagatgaatg gcttccacgt
2221 ggtgattgct atgttaaagc acaaccgaaa ggtgtttctg ttcacttgtt agctggtaat
2281 gttccattat caggagtgac atctattttg cgtgctattt taacaaaaaa tgagtgcatt
2341 attaaaactt cgtcttcaga tccttttact gcaaacgctt tagtttccag ttttattgat
2401 gttaatgcag accatccaat aaccaaatca atgtctgtta tgtattggcc gcatgatgaa
2461 gatatgactc tatctcaaag aataatgaat catgccgacg tggttattgc ttggggtgga
2521 gacgaggcga ttaaatgggc ggtaaaatat tcaccaccgc atgtcgatat tctgaaattt
2581 ggaccaaaga aaagcttaag tattattgaa gctcctaaag atatagaagc cgcagcaatg
2641 ggggttgctc atgatatttg tttctatgac cagcaagcct gcttctctac tcaagacgtt
2701 tattatatag gagataattt acctttattt ttaaatgaac ttgaaaaaca gctagatcga
2761 tacgcgaaaa ttttaccaaa aggttcaaat agttttgatg aaaaagcggc gtttactctt
2821 actgaaaaag aaagtctatt tgctggatat gaagtgagaa agggagataa gcaagcttgg
2881 ttaatagtcg tatcacctac aaatagcttt ggaaatcaac cgctatcacg aagtgtgtat
2941 gttcatcaag tatctgatat taaagagata attccttttg ttaataaaaa tagaacacaa
3001 actgtttcta tttatccttg ggaagcgtca ttaaaatatc gagataaatt agcaagaagt
3061 ggagttgaaa gaattgttga atcaggcatg aataatattt tcagagttgg aggggctcat
3121 gattcattat ctcctctcca gtacctagtt aggtttgtat cgcatgagag accatttaat
3181 tatacgacaa aagatgttgc ggttgaaatc gaacaaacac gttacttaga ggaagataag
3241 tttttagttt ttgtcccata gttaaaggaa attatatgaa agatgaaagt gcttttttta
3301 cgattgatca catcatcaag cttgataatg gtcagtctat ccgagtttgg gaaacactcc
3361 ctaaaaagaa cgtaccagag aaaaaacata caatacttat tgcttcgggt tttgctagaa
3421 gaatggatca ttttgcaggt cttgctgagt atttatctac taacggtttt catgtcattc
3481 gctacgattc tttgcatcat gttggattaa gcagtggatg tataaatgaa tttacgatgt
3541 cgattggaaa aaatagcctg cttacagtcg tagattggct tacagatcat ggtgtcgaac
3601 gaatagggct gattgctgct agtttgtcag cgagaatcgc ctatgaggta gtaaataaaa
3661 ttaaattatc atttttaatt acggccgtag gtgtcgttaa tcttagagat acattagaaa
3721 aagcattgga gtatgactat ttgcaattac ctatttcaga gttaccagaa gatcttgact
3781 ttgaaggtca taatttagga tcggaggtct ttgttacaga ttgctttaaa cataattggg
3841 acacgttaga ctcgacactt aatagtgtta aaggattagc gattccattt attgctttta
3901 ctgcaaacga tgatagttgg gtaaagcaaa gtgaagttat agagctcatt gatagcattg
3961 aatctagtaa ttgtaagctc tattcgctaa ttggaagttc acatgatctt ggggaaaatt
4021 tggttgtatt aagaaatttt tatcaatcag tgacgaaggc agccttagca ttagatgatg
4081 gtttattgga tttagagata gacattattg aacctcgatt tgaggacgtt acaagtatta
4141 ctgttaagga gcgtagatta aaaaatgaaa ttgaaaatga attattagaa ttggcttaaa
4201 taaacagaat caccaaaaag gaatagagta tgaagtttgg aaatatttgt ttttcgtatc
4261 aaccaccagg tgaaactcat aagcaagtaa tggatcgctt tgttcggctt ggtatcgcct
4321 cagaagaggt agggtttgat acatattgga ccttagaaca tcattttaca gagtttggtc
4381 ttacgggaaa tttatttgtt gctgcggcta acctgttagg aagaactaaa acattaaatg
4441 ttggcactat gggggttgtt attccgacag cacacccagt tcgacagtta gaagacgttt
4501 tattattaga tcaaatgtcg aaaggtcgtt ttaattttgg aaccgttcga gggctatacc
4561 ataaagattt tcgagtattt ggtgttgata tggaagagtc tcgagcaatt actcaaaatt
4621 tctaccagat gataatggaa agcttacaga caggaaccat tagctctgat agtgattaca
4681 ttcaatttcc taaggttgat gtatatccca aagtgtactc aaaaaatgta ccaacctgta
4741 tgactgctga gtccgcaagt acgacagaat ggctagcaat acaagggcta ccaatggttc
4801 ttagttggat tattggtact aatgaaaaaa aagcacagat ggaactctat aatgaaattg
4861 cgacagaata tggtcatgat atatctaaaa tagatcattg tatgacttat atttgttctg
4921 ttgatgatga tgcacaaaag gcgcaagatg tttgtcggga gtttctgaaa aattggtatg
4981 actcatatgt aaatgcgacc aatatcttta atgatagcaa tcaaactcgt ggttatgatt
5041 atcataaagg tcaatggcgt gattttgttt tacaaggaca tacaaacacc aatcgacgtg
5101 ttgattatag caatggtatt aaccctgtag gcactcctga gcagtgtatt gaaatcattc
5161 aacgtgatat tgatgcaacg ggtattacaa acattacatg cggatttgaa gctaatggaa
5221 ctgaagatga aataattgct tccatgcgac gctttatgac acaagtcgct cctttcttaa
5281 aagaacctaa ataaattact tatttgatac tagagataat aaggaacaag ttatgaaatt
5341 tggattattt tttctaaact ttcagaaaga tggaataaca tctgaagaaa cgttggataa
5401 tatggtaaag actgtcacgt taattgattc aactaaatat cattttaata ctgcctttgt
5461 taatgaacat cacttttcaa aaaatggtat tgttggagca cctattaccg cagctggttt
5521 tttattaggg ttaacaaata aattacatat tggttcatta aatcaagtaa ttaccaccca
5581 tcaccctgta cgtgtagcag aagaagccag tttattagat caaatgtcag agggacgctt
5641 cattcttggt tttagtgact gcgaaagtga tttcgaaatg gaatttttta gacgtcatat
5701 ctcatcaagg caacaacaat ttgaagcatg ctatgaaata attaatgacg cattaactac
5761 aggttattgt catccccaaa acgactttta tgattttcca aaggtttcaa ttaatccaca
5821 ctgttacagt gagaatggac ctaagcaata tgtatccgct acatcaaaag aagtcgtcat
5881 gtgggcagcg aaaaaggcac tgcctttaac atttaagtgg gaggataatt tagaaaccaa
5941 agaacgctat gcaattctat ataataaaac agcacaacaa tatggtattg atatttcgga
6001 tgttgatcat caattaactg taattgcgaa cttaaatgct gatagaagta cggctcaaga
6061 agaagtgaga gaatacttaa aagactatat cactgaaact taccctcaaa tggacagaga
6121 tgaaaaaatt aactgcatta ttgaagagaa tgcagttggg tctcatgatg actattatga
6181 atcgacaaaa ttagcagtgg aaaaaacagg gtctaaaaat attttattat cctttgaatc
6241 aatgtccgat attaaagatg taaaagatat tattgatatg ttgaaccaaa aaatcgaaat
6301 gaatttacca taataaaatt aaaggcaatt tctatattag attgcctttt taaatttctg
6361 ttgatattag gtattactgg agagggtatg actgtccata ctgaatataa aagaaatcaa
6421 atcattgcta gttcagaaat tgatgatctt atctttatga cgaaaccaca agagtggtca
6481 tttgaagagc aaaaagaaat acgggataaa ttagttcgtg aggcttttta ttttcactac
6541 aatagaaatg aagaatatag aaattattgt atcaatcagc atgtgagtga taatttacac
6601 actattgatg aaatacccgt gtttccaaca tctgttttta aatataagaa attacatact
6661 gtcacagccg aggacattga aaattggtat acaagtagtg gaactcgtgg agtaaaaagt
6721 catattgcac gtgatcgtct tagcattgaa cgcttgcttg gttctgtcaa cttcggaatg
6781 aaatacgttg gagattggtt tgagcatcaa atggaattga taaatttagg accagataga
6841 ttcaatacaa ataatatttg gtttaaatat gtcatgagtt tggtcgagtt actttatccg
6901 actgaattta cagttgataa tgacaaaata gattttgaaa aaacagtaaa acatctattt
6961 agaattaaga atagtaaaaa agacatttgc ttaattgggc caccattttt tgtgtatctt
7021 ttgtgccaat atatgaaaga aaacaatatt gaatttaaag gaggagatag agtacatatt
7081 attactggtg gaggatggaa atctaatcag aatgactctt tagatcgtgc tgattttaat
7141 caattattaa tggatacttt ccaactcgac aagattaatc aaattagaga tacctttaat
7201 caagttgagc ttaatacttg tttttttgaa gatgaatttc aaagaaaaca tgttccaccg
7261 tgggtatatg ctcgggctct tgatcctgaa accttgaaac ccgtagcaga tggtgagatc
7321 gggttgttaa gttatatgga cgcctcatca actgcttacc ctgcttttat tgttactgat
7381 gatatcggta ttgtaaaaga aattagagaa ccagatcctt acccaggggt aactgttgag
7441 attgttcggc gcttaaatac acgtgcgcaa aaaggatgcg cgctctctat ggctaatgtc
7501 atacaaaaga atatcaagga ttaagttatg attgttgatg gtagagtttc aaaaatagta
7561 ttagcgtcaa taagaaataa tatatataag gtatttatta ctgtaaattc accaataaag
7621 ttcatcgctg gacaatttgt aatggtcacg attaatggga aaaaatgccc tttttcaatt
7681 gcgaattgcc cgacaaaaaa ttacgaaata gaattgcata ttggtagttc gaatagagac
7741 tgctcattgg atattatcga atattttgtc gatgctcttg ttgaggaagt cgcaattgag
7801 ttagatgctc cccatggaaa cgcttggtta cggtctgaaa gtaataaccc attgctatta
7861 attgcgggag gtacaggttt atcatatata aatagcattc taacaaattg cttaaatagg
7921 aatatacctc aagatattta tctttactgg ggagtaaaag acagttctct tttgtatgaa
7981 gatgaagagt tactaaactt atcactaaac aacaaaaact ttcattatat tcctgttatt
8041 gaagataaaa gtgaagaatg gatagggaga aaaggcactg ttcttgatgc tgtcatggaa
8101 gattttactg atcttactta ttttgatatt tatgtttgtg gacccttcat gatggctaaa
8161 acagcaaaag aaaaattaat tgaagagaaa aaagcaaagt cagaacagat gtttgccgat
8221 gcttttgcat acgtataaag agaatataaa aagccagatt attaatccgg cttttttatt
8281 atttcaatgt gtccaagggt aaccaagtta cttcaactcc cgcctgagag aacatatctt
8341 ggctcacttg aattttttca ccccaacgag ataagaagtc ttcagtttgt tcagggcaat
8401 atacttttga tattcctgtt tggatgattt ttgctgcgca gtttggacaa gggaagtgag
8461 taacccaaat atcgcaacct tctaaatcac gcttggcaaa taaaatggca ttttcttcag
8521 catgaagtgt ttttaaatat ttaatttcac gttcatctgt atctgcactg tctgatacac
8581 catgtgggta gccattaaat ccaacagaaa caatacggtt atgcttggtg attactgcac
8641 caacttgagt cgac
//