LOCUS AF132211 6501 bp DNA linear SYN 18-MAY-1999
DEFINITION Cloning vector pFBneo, complete sequence.
ACCESSION AF132211
VERSION AF132211.1
KEYWORDS .
SOURCE Cloning vector pFBneo
ORGANISM Cloning vector pFBneo
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6501)
AUTHORS Felts,K. and Grafsky,A.J.
TITLE Direct Submission
JOURNAL Submitted (01-MAR-1999) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
FEATURES Location/Qualifiers
source 1..6501
/organism="Cloning vector pFBneo"
/mol_type="genomic DNA"
/host="Escherichia coli"
/db_xref="taxon:93464"
repeat_region 209..760
/note="5'"
/rpt_type=long_terminal_repeat
misc_feature 760..2046
/note="Psi+; extended viral packaging signal"
misc_feature 2057..2086
/note="multiple cloning site"
misc_feature 2092..2680
/note="IRES"
gene 2678..3487
/gene="neo"
CDS 2678..3487
/gene="neo"
/function="neomycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside 3'-phosphotransferase"
/protein_id="AAD31025.1"
/translation="MGSFRMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
SAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEV
PGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQ
DDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
repeat_region complement(3547..4140)
/note="3'"
/rpt_type=long_terminal_repeat
rep_origin 4253
gene complement(5436..6296)
/gene="bla"
CDS complement(5436..6296)
/gene="bla"
/function="ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAD31024.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1504 a 1805 c 1669 g 1523 t
ORIGIN
1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
121 ccctattccc cacactcacc ggagccaaag ccgcgggaca tatacatgtg aaagacccca
181 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
241 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
301 caaagcggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
361 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
421 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
481 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
541 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
601 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
661 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
721 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
781 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
841 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
901 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc
961 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg
1021 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag gagggatatg
1081 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
1141 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc
1201 tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgggccag actgttacca
1261 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
1321 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
1381 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
1441 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
1501 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
1561 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
1621 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga
1681 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
1741 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
1801 ggagacctct ggcggcacgt accaagaaca actggaccga ccggtggtac ctcaccctta
1861 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg
1921 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc
1981 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag
2041 actgccggat cgaattgtcg acgaattcgg atcctcgagc ggccgcgatc cggttatttt
2101 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
2161 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
2221 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
2281 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
2341 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
2401 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
2461 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
2521 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
2581 acgatgataa tatggaacaa aaacttattt ctgaagaaga cttggacacc aaactttcct
2641 gccgctcgat ttctccaccc tggtgccacg cggttccatg ggatcgtttc gcatgattga
2701 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
2761 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
2821 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
2881 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
2941 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
3001 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
3061 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
3121 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
3181 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
3241 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
3301 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
3361 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
3421 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
3481 cttctgagcg ggactctggg gttcgataaa ataaaagatt ttatttagtc tccagaaaaa
3541 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
3601 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
3661 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
3721 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
3781 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
3841 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
3901 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
3961 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
4021 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
4081 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
4141 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
4201 gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
4261 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
4321 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
4381 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
4441 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
4501 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
4561 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
4621 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
4681 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
4741 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
4801 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
4861 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
4921 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
4981 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
5041 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
5101 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
5161 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
5221 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
5281 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
5341 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
5401 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
5461 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
5521 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
5581 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
5641 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
5701 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
5761 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
5821 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
5881 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
5941 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
6001 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
6061 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
6121 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
6181 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
6241 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
6301 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
6361 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
6421 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
6481 acgaggccct ttcgtcttca a
//