LOCUS AF032970 4228 bp DNA linear BCT 24-JUL-2016
DEFINITION Pseudomonas putida inducible histidine transporter (hutT),
imidazolone propionate hydrolase (hutI), and N-formylglutamate
amidohydrolase (hutG) genes, complete cds; and proline
iminopeptidase (pip1) gene, partial cds.
ACCESSION AF032970
VERSION AF032970.1
KEYWORDS .
SOURCE Pseudomonas putida
ORGANISM Pseudomonas putida
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 4228)
AUTHORS Phillips,A.T. and Baker,C.S.
TITLE Inducible Histidine transporter from Pseudomonas putida
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4228)
AUTHORS Phillips,A.T. and Baker,C.S.
TITLE Direct Submission
JOURNAL Submitted (04-NOV-1997) Biochemistry and Molecular Biology,
Pennsylvania State University, Althouse Laboratory, University
Park, PA 16802, USA
FEATURES Location/Qualifiers
source 1..4228
/organism="Pseudomonas putida"
/mol_type="genomic DNA"
/strain="ATCC 12633"
/type_material="type strain of Pseudomonas putida"
/db_xref="taxon:303"
gene 193..1596
/gene="hutT"
/note="histidine utilization"
CDS 193..1596
/gene="hutT"
/codon_start=1
/transl_table=11
/product="inducible histidine transporter"
/protein_id="AAB86967.1"
/translation="MQQAQGLKRGLNARHIRFMALGSAIGTGLFYGSASAIQMAGPAV
LLAYLIGGAAVFMVMRALGEMAVHNPVAGSFGHYASTYLGPMAGFILGWTYAFEMVIV
AIADVTAFGIYMGFWFPEVARWIWVLGIVFLIGGLNLCNVKVFGEMEFWLSLLKVGAI
VAMILAGLGIMAFGFSQVGTGHAVGVSNLFDHGGFMPNGVGGLIASFAVVMFAFGGIE
IIGVTAGEAKDPQRVIPKAINAVPLRILLFYVLTLFVLMCLYPWPQIGSEGSPFVQIF
SNLGIGSAAAVLNVVVISAAISAINSDIFGAGRMMYGLAHQGHAPRSFGKLSKHGVPW
MTVVVMGAALLIGVLLNYLIPENVFLLIASIATFATVWVWLMILLTQVAMRRSMSREE
VAQLKFPVPFWPYGPAMAIAFMVFIFGVLGYFPDTQAALIVGVIWVVFLVASYLLWCK
PRAGQGQPVAEPVELHR"
gene 1612..2814
/gene="hutI"
/note="histidine utilization"
CDS 1612..2814
/gene="hutI"
/codon_start=1
/transl_table=11
/product="imidazolone propionate hydrolase"
/protein_id="AAB86968.1"
/translation="MRTVWQHCHVATMAEGRYSAIEDAAIVTSAGLIEWIGPRAELAP
VDADRTVDLGGAWITPGLIDCHTHACSVATAVASSSSACKASVRRNRRTGWRYRQYRA
GHPRGQRGRVVRQCPPAGAGADARWCDHLEIKSGYGLDLANERKMLRVARRLADELPL
AVRATCLAAHALPPEYAGRADDYIAHICDEMLPALAAEGLVDAVDAFCEHLAFSPAQV
ERLFIKARELGLPVKLHAEQLSSLHGSSLAARYQALSADHLEFMTEEDAIAMAAAGTV
AVLLPGAFYFLRETQLPPMDALRRHGVKIALASDLNPGTSPGLSLRLMLNMGCTCFRM
TPEEALAGVTVHAATALGLGDSHGSLEVGKVADFVAWQIERPADLAYWLGGDLPKRVV
RKGHEISN"
gene 2825..3628
/gene="hutG"
/note="histidine utilization"
CDS 2825..3628
/gene="hutG"
/codon_start=1
/transl_table=11
/product="N-formylglutamate amidohydrolase"
/protein_id="AAB86969.1"
/translation="MDKVLSFHQGRLPLLISMPHAGLRLSDAVRDGLVEEARSLPDTD
WHIPQLYDFARDLGASVVAAEYSRFVIDLNRPDDDKPLYAGATTGLYPATLFEGEPLF
KEGLAPSGEERKRYLEQIWRPYHGTLRRELDRLREQFGYALLWDAHSIRSHIPHLFDG
KLPDFNLGTFNGASCDPVLAERLQGVCAEATGYSHVLNGRFKGGHITRHYGDPAKHIH
AVQLELAQSTYMEETEPFTYREDLAQPTQVVLKQLLQALLAWGAERYQR"
gene 3665..>4228
/gene="pip1"
CDS 3665..>4228
/gene="pip1"
/codon_start=1
/transl_table=11
/product="proline iminopeptidase"
/protein_id="AAB86970.1"
/translation="MMPNGSQYPHTECAMQTLYPQIKPYARHDLAVEAPHVLYVDESG
SPEGLPVVFIHGGPGAGCDDQSRCYFDPNLYRIITFDQRGCGRPRPMPAWRTTQPGTW
SRTWSASASTWASTNGCCSAARGVRPWPWPYARPPERVHGLICGASSCAGRRRSSGST
RRRQPPVPRLLAGLHRADSAGRTRRPGQ"
BASE COUNT 711 a 1365 c 1351 g 801 t
ORIGIN
1 ttgaccggtt tgctgccggc aggtgtgctg ccaagcctgt aatgccccct ggggccgctg
61 tgcggcccat tcgcagcaca aggctgctcc tgcaaaagcg accgctccct tgtacgagca
121 gccttgtgct gcgaaagggc tgcatgccag ccccctgatc tcacgaccac aaaaacaatt
181 taaggacgtg acatgcaaca agctcaaggt ctcaagcgcg ggctaaatgc ccgccacatc
241 cgtttcatgg ccctcggttc tgccatcgga accgggctgt tctacggctc cgcctcagcc
301 atccagatgg ccggcccggc cgtgctgctg gcttacctga tcggcggtgc cgctgtgttc
361 atggtcatgc gcgccctcgg cgaaatggcc gtgcacaacc cggtggccgg ctccttcggc
421 cactacgcca gtacctacct cggccccatg gccggcttca tcctcggctg gacctacgcc
481 ttcgagatgg taattgtcgc catcgccgac gtcaccgcct tcggtatcta catgggcttc
541 tggttcccgg aagtggcccg ctggatctgg gtgctgggca tcgtcttcct gatcggcggc
601 ctcaacctgt gcaacgtcaa ggtgttcggc gagatggaat tctggctgtc gctgctcaag
661 gttggcgcca tcgtcgcgat gatcctggcc ggcctcggca tcatggcctt cggtttcagc
721 caggtgggta ccgggcatgc cgtcggtgtg agcaacctgt tcgaccatgg cggcttcatg
781 cccaatggcg tgggcgggct gatcgcctcc tttgccgtgg tgatgttcgc cttcggcggt
841 atcgagatca ttggcgtgac tgccggtgaa gccaaggacc cgcagcgggt catccccaag
901 gcaatcaacg ccgtgcccct gcgcattctg ctgttctatg tgctcaccct gttcgtgctg
961 atgtgcctgt acccgtggcc gcagatcggc agcgaaggta gcccgttcgt gcagattttc
1021 agcaacctgg ggattggctc tgccgccgca gtgctgaacg tggtggtaat ttccgctgcg
1081 atctcggcca tcaacagtga catcttcggt gctggccgca tgatgtatgg tctggcccat
1141 caaggccatg cgccacgcag tttcggcaag ctgtcgaaac acggtgtgcc atggatgacc
1201 gtggtggtga tgggcgccgc gctgctgatc ggcgtgctgc tcaactacct gatccctgag
1261 aacgtgttcc tgctgatcgc ttcgatcgcc accttcgcca ccgtgtgggt gtggttgatg
1321 atcctgctca cccaagtggc catgcgccgc agcatgagcc gtgaagaagt ggcccagctc
1381 aagttcccgg taccgttctg gccatacggc ccggccatgg ccattgcgtt catggtcttc
1441 atctttggcg tgctcggcta cttcccggat acccaggcgg cattgatcgt cggcgtgata
1501 tgggtggtgt tcctggtggc gtcctacctg ctgtggtgca agccgcgcgc agggcagggc
1561 cagcccgtag cagagcctgt cgagctgcac cgctagcaaa ggagattgtg catgagaacc
1621 gtctggcagc attgccatgt ggcaaccatg gccgagggcc gttactcggc catcgaagac
1681 gcggccatcg tcaccagcgc cgggctgatc gaatggatcg gcccacgcgc cgagctggcg
1741 ccggtcgacg ctgaccgcac ggtggacctg ggcggcgcct ggatcacccc cgggctgatc
1801 gattgccaca cccacgcgtg ttcggtggca accgcagtgg cgagttcgag cagcgcctgc
1861 aaggcgtcag tacgccgaaa tcgccgcaca gggtggcggt atcgccagta ccgtgcgggc
1921 cacccgcgcg gccagcgagg acgagttgtt cgccagtgcc cgccagcggg tgcaggcgct
1981 gatgcgcgat ggtgtgacca cctcgagatc aagtccggct atggcctgga cctggccaac
2041 gagcgcaaga tgctgcgcgt ggcccggcgc cttgccgacg agctgccgct ggcggtgcgc
2101 gccacctgcc tggccgcgca cgccctgccg ccggagtacg ccggccgggc cgacgactac
2161 atcgcgcaca tctgtgacga gatgctgccg gccctggccg ccgaaggcct ggtggatgcg
2221 gtggatgcct tctgcgaaca cctggcgttc tccccggccc aggtggagcg gctgttcatc
2281 aaggcgcgtg agctgggcct gccggtcaag ctgcacgccg aacagctgtc gtcgctgcat
2341 ggctcgagcc tggcggcccg ctaccaggcg ctgtcggccg accacctgga gttcatgacc
2401 gaggaagacg ccattgccat ggccgcagcc ggcacggtcg ccgtgctgct accgggcgcc
2461 ttctatttct tgcgtgaaac ccagctgcca ccgatggatg cattgcgccg ccacggggtg
2521 aagattgccc tggccagcga cctcaacccc ggcacctcgc cagggctgtc gctgcggctg
2581 atgctgaaca tgggctgcac gtgtttccgc atgacgccgg aagaagcctt ggccggcgtc
2641 accgtgcatg ccgccacggc gttgggcctg ggcgacagcc acggctcgct ggaagtgggc
2701 aaggtagccg acttcgtcgc ctggcaaatc gaacgccccg ccgacttggc ctactggctg
2761 ggtggcgacc tgcccaagcg cgtggtgcgc aagggccacg aaatatccaa ctgagcgagg
2821 cacgatggac aaggttctga gttttcacca aggccgcctg ccgctgctga tcagcatgcc
2881 ccacgccggc ctgcgcctga gcgacgccgt gcgtgatggc ctggtcgagg aggcgcgcag
2941 cttgccggac accgactggc acatcccgca gctgtacgac tttgcccgcg acctgggcgc
3001 cagcgtggta gccgcagagt actcgcgttt cgtcatcgac ctgaaccgcc cggatgacga
3061 caagccgctt tacgccggcg ccaccactgg cctgtacccg gccacgttgt tcgaaggcga
3121 gccgctgttc aaggaggggt tggcgccgtc tggcgaagaa cgcaaacgct acctggaaca
3181 gatctggcgc ccgtatcacg gcacccttcg gcgcgaactg gaccggttgc gcgagcagtt
3241 tggctatgcc ctgttgtggg atgcccactc gatccgctcg cacatcccgc acctgttcga
3301 tggcaagttg ccggacttca acctgggtac cttcaatggc gccagctgcg atccggtgct
3361 ggccgagcgg ttgcagggcg tgtgcgccga agcgacaggt tacagtcatg tgttgaatgg
3421 tcggttcaaa ggcggacaca tcacccggca ctatggtgac cccgcgaagc atatccatgc
3481 ggtgcagctg gagttggcgc aaagcacgta catggaggaa accgagccgt ttacctaccg
3541 ggaagacctg gcgcaaccga cgcaggtggt tctgaagcag cttttgcaag cgctgctggc
3601 ttggggggca gaacgatacc agcgttgagt ggaagaggcg gtgctcaaag cgcaattcag
3661 gtttatgatg cccaacggca gtcaatatcc tcacacggag tgcgcaatgc agaccctcta
3721 cccgcagatc aaaccctacg cccggcacga tctggccgtg gaagcgccgc atgtgctgta
3781 tgtcgatgaa agcggctcgc cggaaggtct gcccgtggta ttcatccacg gtggcccggg
3841 tgctggctgc gacgaccaga gccgttgcta ctttgacccc aacctgtacc gcatcatcac
3901 cttcgaccag cgcggctgtg gccgtccacg ccccatgcca gcctggagaa caacacaacc
3961 tggcacctgg tcgaggacct ggagcgcatc cgcgagcacc tgggcatcga caaatgggtg
4021 ctgttcggcg gctcgtgggg ttcgaccctg gccctggccc tacgccagac cacccgagcg
4081 tgtgcatggg ctgatctgcg gggcatcttc ctgtgccggc cgcaggagat cgagtggttc
4141 taccaggagg cgccagccgc ctgttccccg actactggca ggactacatc gcgccgattc
4201 cgccggaaga acgcggcgac ctggtcaa
//