LOCUS AF027960 5565 bp DNA circular SYN 01-JUL-1998
DEFINITION Expression vector pIB3, integrating vector for constitutive gene
expression in Pichia pastoris, complete plasmid sequence.
ACCESSION AF027960
VERSION AF027960.1
KEYWORDS .
SOURCE Expression vector pIB3
ORGANISM Expression vector pIB3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5565)
AUTHORS Sears,I.B., O'Connor,J., Rossanese,O.W. and Glick,B.S.
TITLE A versatile set of vectors for constitutive and regulated gene
expression in Pichia pastoris
JOURNAL Yeast 14 (8), 783-790 (1998)
PUBMED 9675822
REFERENCE 2 (bases 1 to 5565)
AUTHORS Sears,I.B., O'Connor,J., Rossanese,O.W. and Glick,B.S.
TITLE Direct Submission
JOURNAL Submitted (03-OCT-1997) Molecular Genetics and Cell Biology,
University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
FEATURES Location/Qualifiers
source 1..5565
/organism="Expression vector pIB3"
/mol_type="genomic DNA"
/host="Pichia pastoris"
/db_xref="taxon:69275"
/note="integrating vector for constitutive gene expression
in Pichia pastoris; has modified pUC19 polylinker;
contains the moderately active constitutive promoter from
the Pichia pastoris YPT1 gene"
CDS complement(1851..2711)
/codon_start=1
/product="beta-lactamase"
/protein_id="AAC53723.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
gene 2811..5342
/gene="HIS4"
CDS 2811..5342
/gene="HIS4"
/codon_start=1
/product="histidinol dehydrogenase"
/protein_id="AAC53722.1"
/translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQST
ELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYR
TLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGII
PIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSS
KKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGF
CHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAE
AKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQ
PKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQ
NGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLA
ETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASP
PKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTA
AKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVVSDLLSQAEHGIDSQVILLAV
DMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLI
LQIENASYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQK
FITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
BASE COUNT 1462 a 1263 c 1322 g 1518 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgat gagtcacaat ctgcttccac agacgagtac aaggacaggc aaaaggaatt
241 ggaagaagtt gctaacccaa taatgagcaa gttctatgga gctgctggtg gagctcctgg
301 tggagctcct ggtggcttcc ctggaggttt ccctggcgga gctggcgcag ctggcggtgc
361 cccaggtggt gctgccccag gcggagacag cggaccaacc gtggaagaag tcgattaagc
421 aattcaacgg ataaattctg gttaatatat ataacgtgaa taggaaatta aggaaatttt
481 ggatctaata atgtgctgta tgccgacatc gggcatcgta gattgtatag tatcgctgac
541 actataataa gccagccaaa acccctaaac cagttgccct ccactaatta gtgtactacc
601 caatcttgcc tcttcgggtg tcttttataa ggacagattc acaagctctt gttgcccaat
661 acacacatac acacagagat aatagcagtc gaattcgagc tcggtacccg gggatccact
721 agtctcgagc tgcaggcatg caagcttctt agacatgact gttcctcagt tcaagttggg
781 cacttacgag aagaccggtc ttgctagatt ctaatcaaga ggatgtcaga atgccatttg
841 cctgagagat gcaggcttca tttttgatac ttttttattt gtaacctata tagtatagga
901 ttttttttgt cattttgttt cttctcgtac gagcttgctc ctgatcagcc tatctcgcag
961 ctgatgaata tcttgtggta ggggtttggg aaaatcattc gagtttgatg tttttcttgg
1021 tatttcccac acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
1081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
1141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
1201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
1261 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
1321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
1381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
1441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
1501 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
1561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
1621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
1681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
1741 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
1801 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
1861 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
1921 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
1981 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
2041 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
2101 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
2161 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
2221 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
2281 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
2341 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
2401 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
2461 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
2521 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
2581 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
2641 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
2701 tgaatactca tactcttcct ttttcaatga tctcctgatg actgactcac tgataataaa
2761 aatacggctt cagaatttct caagactaca ctcactgtcc gacttcaagt atgacatttc
2821 ccttgctacc tgcatacgca agtgttgcag agtttgataa ttccttgagt ttggtaggaa
2881 aagccgtgtt tccctatgct gctgaccagc tgcacaacct gatcaagttc actcaatcga
2941 ctgagcttca agttaatgtg caagttgagt catccgttac agaggaccaa tttgaggagc
3001 tgatcgacaa cttgctcaag ttgtacaata atggtatcaa tgaagtgatt ttggacctag
3061 atttggcaga aagagttgtc caaaggatga tcccaggcgc tagggttatc tataggaccc
3121 tggttgataa agttgcatcc ttgcccgcta atgctagtat cgctgtgcct ttttcttctc
3181 cactgggcga tttgaaaagt ttcactaatg gcggtagtag aactgtttat gctttttctg
3241 agaccgcaaa gttggtagat gtgacttcca ctgttgcttc tggtataatc cccattattg
3301 atgctcggca attgactact gaatacgaac tttctgaaga tgtcaaaaag ttccctgtca
3361 gtgaaatttt gttggcgtct ttgactactg accgccccga tggtctattc actactttgg
3421 tggctgactc ttctaattac tcgttgggcc tggtgtactc gtccaaaaag tctattccgg
3481 aggctataag gacacaaact ggagtctacc aatctcgtcg tcacggtttg tggtataaag
3541 gtgctacatc tggagcaact caaaagttgc tgggtatcga attggattgt gatggagact
3601 gcttgaaatt tgtggttgaa caaacaggtg ttggtttctg tcacttggaa cgcacttcct
3661 gttttggcca atcaaagggt cttagagcca tggaagccac cttgtgggat cgtaagagca
3721 atgctccaga aggttcttat accaaacggt tatttgacga cgaagttttg ttgaacgcta
3781 aaattaggga ggaagctgat gaacttgcag aagctaaatc caaggaagat atagcctggg
3841 aatgtgctga cttattttat tttgcattag ttagatgtgc caagtacggc gtgacgttgg
3901 acgaggtgga gagaaacctg gatatgaagt ccctaaaggt cactagaagg aaaggagatg
3961 ccaagccagg atacaccaag gaacaaccta aagaagaatc caaacctaaa gaagtccctt
4021 ctgaaggtcg tattgaattg tgcaaaattg acgtttctaa ggcctcctca caagaaattg
4081 aagatgccct tcgtcgtcct atccagaaaa cggaacagat tatggaatta gtcaaaccaa
4141 ttgtcgacaa tgttcgtcaa aatggtgaca aagccctttt agaactaact gccaagtttg
4201 atggagtcgc tttgaagaca cctgtgttag aagctccttt cccagaggaa cttatgcaat
4261 tgccagataa cgttaagaga gccattgatc tctctataga taacgtcagg aaattccatg
4321 aagctcaact agcggagacg ttgcaagttg agacttgccc tggtgtagtc tgctctcgtt
4381 ttgcaagacc tattgagaaa gttggcctct atattcctgg tggaaccgca attctgcctt
4441 ccacttccct gatgctgggt gttcctgcca aagttgctgg ttgcaaagaa attgtttttg
4501 catctccacc taagaaggat ggcaccctta ccccagaagt catctacgtt gcccacaagg
4561 ttggtgctaa gtgtatcgtg ctagcaggag gcgcccaggc agtagctgct atggcttacg
4621 gaacagaaac tgttcctaag tgtgacaaaa tatttggtcc aggaaaccag ttcgttactg
4681 ctgccaagat gatggttcaa aatgacacat cagccctgtg tagtattgac atgcctgctg
4741 ggccttctga agttctagtt attgctgata aatacgctga tccagatttc gttgtctcag
4801 accttctgtc tcaagctgaa catggtattg attcccaggt gattctgttg gctgtcgata
4861 tgacagacaa ggagcttgcc agaattgaag atgctgttca caaccaagct gtgcagttgc
4921 caagggttga aattgtacgc aagtgtattg cacactctac aaccctatcg gttgcaacct
4981 acgagcaggc tttggaaatg tccaatcagt acgctcctga acacttgatc ctgcaaatcg
5041 agaatgcttc ttatgttgat caagtacaac acgctggatc tgtgtttgtt ggtgcctact
5101 ctccagagag ttgtggagat tactcctccg gcaccaacca cactttgcca acgtacggat
5161 atgcccgtca atacagcgga gttaacactg caaccttcca gaagttcatc acttcacaag
5221 acgtaactcc tgagggactg aaacatattg gccaagcagt gatggatctg gctgctgttg
5281 aaggtctaga tgctcaccgc aatgctgtta aggttcgtat ggagaaactg ggacttattt
5341 aattatttag agattttaac ttacatttag attcgataga tcattattga agcatttatc
5401 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
5461 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
5521 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//