LOCUS AF027958 5270 bp DNA circular SYN 01-JUL-1998
DEFINITION Expression vector pIB1, promoter-free integrating vector for
expressing genes in Pichia pastoris, complete plasmid sequence.
ACCESSION AF027958
VERSION AF027958.1
KEYWORDS .
SOURCE Expression vector pIB1
ORGANISM Expression vector pIB1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5270)
AUTHORS Sears,I.B., O'Connor,J., Rossanese,O.W. and Glick,B.S.
TITLE A versatile set of vectors for constitutive and regulated gene
expression in Pichia pastoris
JOURNAL Yeast 14 (8), 783-790 (1998)
PUBMED 9675822
REFERENCE 2 (bases 1 to 5270)
AUTHORS Sears,I.B., O'Connor,J., Rossanese,O.W. and Glick,B.S.
TITLE Direct Submission
JOURNAL Submitted (03-OCT-1997) Molecular Genetics and Cell Biology,
University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
FEATURES Location/Qualifiers
source 1..5270
/organism="Expression vector pIB1"
/mol_type="genomic DNA"
/host="Pichia pastoris"
/db_xref="taxon:69273"
/note="promoter-free integrating vector for expressing
genes in Pichia pastoris; has modified pUC19 polylinker;
does not contain a yeast promoter; precursor of the
expression vectors pIB2, pIB3, and pIB4"
CDS complement(1556..2416)
/codon_start=1
/product="beta-lactamase"
/protein_id="AAC53719.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
gene 2516..5047
/gene="HIS4"
CDS 2516..5047
/gene="HIS4"
/codon_start=1
/product="histidinol dehydrogenase"
/protein_id="AAC53718.1"
/translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQST
ELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYR
TLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGII
PIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSS
KKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGF
CHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAE
AKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQ
PKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQ
NGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLA
ETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASP
PKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTA
AKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVVSDLLSQAEHGIDSQVILLAV
DMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLI
LQIENASYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQK
FITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
BASE COUNT 1367 a 1199 c 1265 g 1439 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
421 ccactagtct cgagctgcag gcatgcaagc ttcttagaca tgactgttcc tcagttcaag
481 ttgggcactt acgagaagac cggtcttgct agattctaat caagaggatg tcagaatgcc
541 atttgcctga gagatgcagg cttcattttt gatacttttt tatttgtaac ctatatagta
601 taggattttt tttgtcattt tgtttcttct cgtacgagct tgctcctgat cagcctatct
661 cgcagctgat gaatatcttg tggtaggggt ttgggaaaat cattcgagtt tgatgttttt
721 cttggtattt cccacacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
961 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
1021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
1081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
1141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
1201 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
1261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
1321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
1381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
1441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
1501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
1561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
1621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
1681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
1741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
1801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
1861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
1921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
1981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
2041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
2101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
2161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
2221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
2281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
2341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
2401 aatgttgaat actcatactc ttcctttttc aatgatctcc tgatgactga ctcactgata
2461 ataaaaatac ggcttcagaa tttctcaaga ctacactcac tgtccgactt caagtatgac
2521 atttcccttg ctacctgcat acgcaagtgt tgcagagttt gataattcct tgagtttggt
2581 aggaaaagcc gtgtttccct atgctgctga ccagctgcac aacctgatca agttcactca
2641 atcgactgag cttcaagtta atgtgcaagt tgagtcatcc gttacagagg accaatttga
2701 ggagctgatc gacaacttgc tcaagttgta caataatggt atcaatgaag tgattttgga
2761 cctagatttg gcagaaagag ttgtccaaag gatgatccca ggcgctaggg ttatctatag
2821 gaccctggtt gataaagttg catccttgcc cgctaatgct agtatcgctg tgcctttttc
2881 ttctccactg ggcgatttga aaagtttcac taatggcggt agtagaactg tttatgcttt
2941 ttctgagacc gcaaagttgg tagatgtgac ttccactgtt gcttctggta taatccccat
3001 tattgatgct cggcaattga ctactgaata cgaactttct gaagatgtca aaaagttccc
3061 tgtcagtgaa attttgttgg cgtctttgac tactgaccgc cccgatggtc tattcactac
3121 tttggtggct gactcttcta attactcgtt gggcctggtg tactcgtcca aaaagtctat
3181 tccggaggct ataaggacac aaactggagt ctaccaatct cgtcgtcacg gtttgtggta
3241 taaaggtgct acatctggag caactcaaaa gttgctgggt atcgaattgg attgtgatgg
3301 agactgcttg aaatttgtgg ttgaacaaac aggtgttggt ttctgtcact tggaacgcac
3361 ttcctgtttt ggccaatcaa agggtcttag agccatggaa gccaccttgt gggatcgtaa
3421 gagcaatgct ccagaaggtt cttataccaa acggttattt gacgacgaag ttttgttgaa
3481 cgctaaaatt agggaggaag ctgatgaact tgcagaagct aaatccaagg aagatatagc
3541 ctgggaatgt gctgacttat tttattttgc attagttaga tgtgccaagt acggcgtgac
3601 gttggacgag gtggagagaa acctggatat gaagtcccta aaggtcacta gaaggaaagg
3661 agatgccaag ccaggataca ccaaggaaca acctaaagaa gaatccaaac ctaaagaagt
3721 cccttctgaa ggtcgtattg aattgtgcaa aattgacgtt tctaaggcct cctcacaaga
3781 aattgaagat gcccttcgtc gtcctatcca gaaaacggaa cagattatgg aattagtcaa
3841 accaattgtc gacaatgttc gtcaaaatgg tgacaaagcc cttttagaac taactgccaa
3901 gtttgatgga gtcgctttga agacacctgt gttagaagct cctttcccag aggaacttat
3961 gcaattgcca gataacgtta agagagccat tgatctctct atagataacg tcaggaaatt
4021 ccatgaagct caactagcgg agacgttgca agttgagact tgccctggtg tagtctgctc
4081 tcgttttgca agacctattg agaaagttgg cctctatatt cctggtggaa ccgcaattct
4141 gccttccact tccctgatgc tgggtgttcc tgccaaagtt gctggttgca aagaaattgt
4201 ttttgcatct ccacctaaga aggatggcac ccttacccca gaagtcatct acgttgccca
4261 caaggttggt gctaagtgta tcgtgctagc aggaggcgcc caggcagtag ctgctatggc
4321 ttacggaaca gaaactgttc ctaagtgtga caaaatattt ggtccaggaa accagttcgt
4381 tactgctgcc aagatgatgg ttcaaaatga cacatcagcc ctgtgtagta ttgacatgcc
4441 tgctgggcct tctgaagttc tagttattgc tgataaatac gctgatccag atttcgttgt
4501 ctcagacctt ctgtctcaag ctgaacatgg tattgattcc caggtgattc tgttggctgt
4561 cgatatgaca gacaaggagc ttgccagaat tgaagatgct gttcacaacc aagctgtgca
4621 gttgccaagg gttgaaattg tacgcaagtg tattgcacac tctacaaccc tatcggttgc
4681 aacctacgag caggctttgg aaatgtccaa tcagtacgct cctgaacact tgatcctgca
4741 aatcgagaat gcttcttatg ttgatcaagt acaacacgct ggatctgtgt ttgttggtgc
4801 ctactctcca gagagttgtg gagattactc ctccggcacc aaccacactt tgccaacgta
4861 cggatatgcc cgtcaataca gcggagttaa cactgcaacc ttccagaagt tcatcacttc
4921 acaagacgta actcctgagg gactgaaaca tattggccaa gcagtgatgg atctggctgc
4981 tgttgaaggt ctagatgctc accgcaatgc tgttaaggtt cgtatggaga aactgggact
5041 tatttaatta tttagagatt ttaacttaca tttagattcg atagatcatt attgaagcat
5101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
5161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
5221 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//