LOCUS       LC177185                 587 bp    DNA     linear   ENV 19-JAN-2018
DEFINITION  Uncultured euryarchaeote mcrA gene for methyl coenzyme M reductase
            alpha subunit, partial cds.
ACCESSION   LC177185
VERSION     LC177185.1
KEYWORDS    ENV.
SOURCE      uncultured euryarchaeote
  ORGANISM  uncultured euryarchaeote
            Archaea; Euryarchaeota; environmental samples.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Ino,K. and Suzuki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2016) to the DDBJ/EMBL/GenBank databases.
            Contact:Kohei Ino
            The University of Tokyo, Graduate School of Science; 7-3-1 Hongo,
            Bunkyo-ku, Tokyo 113-0033, Japan
            URL    :http://www.s.u-tokyo.ac.jp/
REFERENCE   2  
  AUTHORS   Ino,K., Hernsdorf,A.W., Konno,U., Kouduka,M., Yanagawa,K.,
            Kato,S., Sunamura,M., Hirota,A., Togo,Y.S., Ito,K., Fukuda,A.,
            Iwatsuki,T., Mizuno,T., Komatsu,D.D., Tsunogai,U., Ishimura,T.,
            Amano,Y., Thomas,B.C., Banfield,J.F. and Suzuki,Y.
  TITLE     Ecological and genomic profiling of anaerobic methane-oxidizing
            archaea in a deep granitic environment
  JOURNAL   ISME J 12, 31-47 (2018)
  REMARK    DOI:10.1038/ismej.2017.140
COMMENT     
FEATURES             Location/Qualifiers
     source          1..587
                     /clone="MIU_mcrA_3"
                     /collected_by="Graduate School of Science, The University
                     of Tokyo, Geological Survey of Japan, National Institute
                     of Advanced Industrial Science and Technology and Japan
                     Atomic Energy Agency"
                     /collection_date="2012-08-01/2015-12-22"
                     /country="Japan:Gifu, Mizunami, the Mizunami underground
                     research laboratory"
                     /db_xref="taxon:114243"
                     /environmental_sample
                     /isolation_source="deep groundwater"
                     /lat_lon="35.3783 N 137.2383 E"
                     /mol_type="genomic DNA"
                     /organism="uncultured euryarchaeote"
                     /PCR_primers="fwd_seq: gcmatgcarathggwatgtc, rev_seq:
                     tcatkgcrtagttdggrtagt"
     CDS             <1..>587
                     /codon_start=1
                     /gene="mcrA"
                     /product="methyl coenzyme M reductase alpha subunit"
                     /protein_id="BAX04346.1"
                     /transl_table=11
                     /translation="YAAKHAEVIHMGTYLPVRRARGENEPGGIAFGFLADIVQTPRKY
                     PDDPVRQTLEVVAAGAMLYDQIWLGSYMSGGVGFTQYATAAYTDNILDDFCYYGMEYV
                     EDKFGKTGAPNTMDTVLDVASEVNFYSLEQFEEYPALLDTIFGGSQRASIVAAAAGCS
                     TAYATGNAQTGLSGWYLSMYLHKEQHSRLGFYGYD"
BASE COUNT          154 a          151 c          137 g          145 t
ORIGIN      
        1 tacgcagcta agcacgcaga agttatccac atgggtacct acctgcctgt tcgacgtgct
       61 cgtggtgaaa acgaacctgg tggtattgct ttcggattct tagctgacat cgttcagact
      121 ccaaggaaat acccagacga cccagttagg caaaccttag aagttgtggc cgctggagca
      181 atgctctacg accagatctg gctcggttcc tacatgtctg gtggtgttgg atttacccaa
      241 tacgcaaccg ctgcctacac tgacaacatc ctcgacgact tctgttacta cggtatggag
      301 tatgtagaag acaaattcgg taagaccgga gcaccaaaca ccatggacac agtcctagat
      361 gttgcttctg aagttaactt ctactctctc gaacagttcg aagaataccc agcattactg
      421 gataccattt tcggtggatc acagagggca tccattgttg cagccgcagc cggatgttcg
      481 actgcatatg ctaccggaaa cgcacaaact ggtctaagtg gatggtactt atccatgtac
      541 ctgcacaaag aacagcactc caggcttgga ttctacggtt acgactt
//