LOCUS       AK313523                1022 bp    mRNA    linear   HTC 24-MAY-2008
DEFINITION  Homo sapiens cDNA, FLJ94081, Homo sapiens deoxycytidine kinase
            (DCK), mRNA.
ACCESSION   AK313523
VERSION     AK313523.1
KEYWORDS    HTC; HTC_FLI; oligo capping.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
            Chiyoda-ku, Tokyo 101-0044, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
            Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
            Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
            Goshima,N., Nomura,N. and Isogai,T.
  TITLE     NEDO functional analysis of protein and research application
            project
  JOURNAL   Unpublished (2008)
COMMENT     Human cDNA sequencing project focused on splicing variants of mRNA
            in NEDO functional analysis of protein and research application
            project supported by Ministry of Economy, Trade and Industry,
            Japan; cDNA selection for complete cds sequencing: Reverse
            Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
            (Hitachi) and Japan Biological Informatics Consortium, Japan
            (JBIC); cDNA complete cds sequencing: JBIC and Biological
            Information Research Center (BIRC), AIST; cDNA library
            construction: Helix Research Institute supported by Japan Key
            Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
            Research Association for Biotechnology, Japan, Biotechnology
            Center, National Institute of Technology and Evaluation, Japan and
            HRI; cDNA mapping to human genome: Central Research Laboratory,
            Hitachi; evaluation and annotation: REPRORI.
FEATURES             Location/Qualifiers
     source          1..1022
                     /cell_line="NT2"
                     /cell_type="teratocarcinoma"
                     /clone="NT2RI2013931"
                     /clone_lib="NT2RI2"
                     /db_xref="H-InvDB:HIT000432389"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /note="mRNA from NT2 neuronal precursor cells treated
                     2-weeks mitotic inhibitor after 5-weeks retinoic acid
                     (RA) induction."
                     /note="majorly NT2 neuron"
                     /organism="Homo sapiens"
     CDS             240..1022
                     /note="Homo sapiens deoxycytidine kinase (DCK), mRNA"
                     /protein_id="BAG36303.1"
                     /translation="MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQL
                     CEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACL
                     SRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHD
                     WMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLH
                     RTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL"
BASE COUNT          287 a          232 c          252 g          250 t
ORIGIN      
        1 agctagggag cgcggcttga ggagggcggg gccgctccgc aggcccgcca gtgtcctcag
       61 ctgcctccgc gcgccaaagt caaaccccga cacccgccgg cgggccggtg agctcactan
      121 ctgacccggc aggtcaggat ctggcttagc tggcgccgcg agctccagtg ctgctgcacc
      181 cgtggccgcc tcccagccct ctttgccgga cgagctctgg gccgccacaa gactaaggaa
      241 tggccacccc gcccaagaga agctgcccgt ctttctcagc cagctctgag gggacccgca
      301 tcaagaaaat ctccatcgaa gggaacatcg ctgcagggaa gtcaacattt gtgaatatcc
      361 ttaaacaatt gtgtgaagat tgggaagtgg ttcctgaacc tgttgccaga tggtgcaatg
      421 ttcaaagtac tcaagatgaa tttgaggaac ttacaatgtc tcagaaaaat ggtgggaatg
      481 ttcttcagat gatgtatgag aaacctgaac gatggtcttt taccttccaa acatatgcct
      541 gtctcagtcg aataagagct cagcttgcct ctctgaatgg caagctcaaa gatgcagaga
      601 aacctgtatt attttttgaa cgatctgtgt atagtgacag gtatattttt gcatctaatt
      661 tgtatgaatc tgaatgcatg aatgagacag agtggacaat ttatcaagac tggcatgact
      721 ggatgaataa ccaatttggc caaagccttg aattggatgg aatcatttat cttcaagcca
      781 ctccagagac atgcttacat agaatatatt tacggggaag aaatgaagag caaggcattc
      841 ctcttgaata tttagagaag cttcattata aacatgaaag ctggctcctg cataggacac
      901 tgaaaaccaa cttcgattat cttcaagagg tgcctatctt aacactggat gttaatgaag
      961 actttaaaga caaatatgaa agtctggttg aaaaggtcaa agagtttttg agtactttgt
     1021 ga
//