LOCUS       AK313191                1464 bp    mRNA    linear   HTC 24-MAY-2008
DEFINITION  Homo sapiens cDNA, FLJ93690, Homo sapiens dihydrolipoamide
            branched chain transacylase (E2component of branched chain keto
            acid dehydrogenase complex; maplesyrup urine disease) (DBT), mRNA.
ACCESSION   AK313191
VERSION     AK313191.1
KEYWORDS    HTC; HTC_FLI; oligo capping.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
            Chiyoda-ku, Tokyo 101-0044, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
            Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
            Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
            Goshima,N., Nomura,N. and Isogai,T.
  TITLE     NEDO functional analysis of protein and research application
            project
  JOURNAL   Unpublished (2008)
COMMENT     Human cDNA sequencing project focused on splicing variants of mRNA
            in NEDO functional analysis of protein and research application
            project supported by Ministry of Economy, Trade and Industry,
            Japan; cDNA selection for complete cds sequencing: Reverse
            Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
            (Hitachi) and Japan Biological Informatics Consortium, Japan
            (JBIC); cDNA complete cds sequencing: JBIC and Biological
            Information Research Center (BIRC), AIST; cDNA library
            construction: Helix Research Institute supported by Japan Key
            Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
            Research Association for Biotechnology, Japan, Biotechnology
            Center, National Institute of Technology and Evaluation, Japan and
            HRI; cDNA mapping to human genome: Central Research Laboratory,
            Hitachi; evaluation and annotation: REPRORI.
FEATURES             Location/Qualifiers
     source          1..1464
                     /clone="KIDNE2013618"
                     /clone_lib="KIDNE2"
                     /db_xref="H-InvDB:HIT000432057"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /organism="Homo sapiens"
                     /tissue_type="kidney"
     CDS             16..1464
                     /note="Homo sapiens dihydrolipoamide branched chain
                     transacylase (E2component of branched chain keto acid
                     dehydrogenase complex; maplesyrup urine disease) (DBT),
                     mRNA"
                     /protein_id="BAG36008.1"
                     /translation="MAAVRMLRTWSRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPS
                     FKYSHPHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQ
                     SDKASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDE
                     HTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILP
                     PSPKVEIMPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHF
                     GYCDEIDLTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQ
                     NITYKASHNIGIAMDTEQGLIVPNVKNVQICSIFDIATELNRLQKLGSVGQLSTTDLT
                     GGTFTLSNIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSA
                     DHRVIDGATMSRFSNLWKSYLENPAFMLLDLK"
BASE COUNT          453 a          290 c          311 g          410 t
ORIGIN      
        1 attttccggg gtaagatggc tgcagtccgt atgctgagaa cctggagcag gaatgcgggg
       61 aagctgattt gtgttcgcta ttttcaaaca tgtggtaatg ttcatgtttt gaagccaaat
      121 tatgtgtgtt tctttggtta tccttcattc aagtatagtc atccacatca cttcctgaaa
      181 acaactgctg ctctccgtgg acaggttgtt cagttcaagc tctcagacat tggagaaggg
      241 attagagaag taactgttaa agaatggtat gtaaaagaag gagatacagt gtctcagttt
      301 gatagcatct gtgaagttca aagtgataaa gcttctgtta ccatcactag tcgttatgat
      361 ggagtcatta aaaaactcta ttataatcta gacgatattg cctatgtggg gaagccatta
      421 gtagacatag aaacggaagc tttaaaagat tcagaagaag atgttgttga aactcctgca
      481 gtgtctcatg atgaacatac acaccaagag ataaagggcc gaaaaacact ggcaactcct
      541 gcagttcgcc gtctggcaat ggaaaacaat attaagctga gtgaagttgt tggctcagga
      601 aaagatggca gaatacttaa agaagatatc ctcaactatt tggaaaagca gacaggagct
      661 atattgcctc cttcacccaa agttgaaatt atgccacctc caccaaagcc aaaagacatg
      721 actgttccta tactagtatc aaaacctccg gtattcacag gcaaagacaa aacagaaccc
      781 ataaaaggct ttcaaaaagc aatggtcaag actatgtctg cagccctgaa gatacctcat
      841 tttggttatt gtgatgagat tgaccttact gaactggtta agctccgaga agaattaaaa
      901 cccattgcat ttgctcgtgg aattaaactc tcctttatgc ctttcttctt aaaggctgct
      961 tccttgggat tactacagtt tcctatcctt aacgcttctg tggatgaaaa ctgccagaat
     1021 ataacatata aggcttctca taacattggg atagcaatgg atactgagca gggtttgatt
     1081 gtccctaatg tgaaaaatgt tcagatctgc tctatatttg acatcgccac tgaactgaac
     1141 cgcctccaga aattgggctc tgtgggtcag ctcagcacca ctgatcttac aggaggaaca
     1201 tttactcttt ccaacattgg atcaattggt ggtacctttg ccaaaccagt gataatgcca
     1261 cctgaagtag ccattggggc ccttggatca attaaggcca ttccccgatt taaccagaaa
     1321 ggagaagtat ataaggcaca gataatgaat gtgagctggt cagctgatca cagagttatt
     1381 gatggtgcta caatgtcacg cttctccaat ttgtggaaat cctatttaga aaacccagct
     1441 tttatgctac tagatctgaa atga
//