LOCUS       AK312252                1151 bp    mRNA    linear   HTC 24-MAY-2008
DEFINITION  Homo sapiens cDNA, FLJ92547, Homo sapiens guanine nucleotide
            binding protein (G protein), alphainhibiting activity polypeptide
            3 (GNAI3), mRNA.
ACCESSION   AK312252
VERSION     AK312252.1
KEYWORDS    HTC; HTC_FLI; oligo capping.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
            Chiyoda-ku, Tokyo 101-0044, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
            Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
            Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
            Goshima,N., Nomura,N. and Isogai,T.
  TITLE     NEDO functional analysis of protein and research application
            project
  JOURNAL   Unpublished (2008)
COMMENT     Human cDNA sequencing project focused on splicing variants of mRNA
            in NEDO functional analysis of protein and research application
            project supported by Ministry of Economy, Trade and Industry,
            Japan; cDNA selection for complete cds sequencing: Reverse
            Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
            (Hitachi) and Japan Biological Informatics Consortium, Japan
            (JBIC); cDNA complete cds sequencing: JBIC and Biological
            Information Research Center (BIRC), AIST; cDNA library
            construction: Helix Research Institute supported by Japan Key
            Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
            Research Association for Biotechnology, Japan, Biotechnology
            Center, National Institute of Technology and Evaluation, Japan and
            HRI; cDNA mapping to human genome: Central Research Laboratory,
            Hitachi; evaluation and annotation: REPRORI.
FEATURES             Location/Qualifiers
     source          1..1151
                     /cell_type="normal astrocytes (NHA5732)"
                     /clone="ASTRO2017253"
                     /clone_lib="ASTRO2"
                     /db_xref="H-InvDB:HIT000431118"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /note="primary culture, normal astrocytes"
                     /organism="Homo sapiens"
     CDS             87..1151
                     /note="Homo sapiens guanine nucleotide binding protein (G
                     protein), alphainhibiting activity polypeptide 3 (GNAI3),
                     mRNA"
                     /protein_id="BAG35184.1"
                     /translation="MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGES
                     GKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARAD
                     DARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLD
                     RISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGV
                     TAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEE
                     KIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQF
                     VFDAVTDVIIKNNLKECGLY"
BASE COUNT          358 a          227 c          284 g          282 t
ORIGIN      
        1 agcaatagac ggtgcctcag cctgccgagc cgcagtttcc gtggtgtgag tgagtccggg
       61 cccgtgtccc ctctcccgcc gccgccatgg gctgcacgtt gagcgccgaa gacaaggcgg
      121 cagtggagcg aagcaagatg atcgaccgca acttacggga ggacggggaa aaagcggcca
      181 aagaagtgaa gctgctgcta ctcggtgctg gagaatctgg taaaagcacc attgtgaaac
      241 agatgaaaat cattcatgag gatggctatt cagaggatga atgtaaacaa tataaagtag
      301 ttgtctacag caatactata cagtccatca ttgcaatcat aagagccatg ggacggctaa
      361 agattgactt tggggaagct gccagggcag atgatgcccg gcaattattt gttttagctg
      421 gcagtgctga agaaggagtc atgactccag aactagcagg agtgattaaa cggttatggc
      481 gagatggtgg ggtacaagct tgcttcagca gatccaggga atatcagctc aatgattctg
      541 cttcatatta tctaaatgat ctggatagaa tatcccagtc taactacatt ccaactcagc
      601 aagatgttct tcggacgaga gtgaagacca caggcattgt agaaacacat ttcaccttca
      661 aagacctata cttcaagatg tttgatgtag gtggccaaag atcagaacga aaaaagtgga
      721 ttcactgttt tgagggagtg acagcaatta tcttctgtgt ggccctcagt gattatgacc
      781 ttgttctggc tgaggacgag gagatgaacc gaatgcatga aagcatgaaa ctgtttgaca
      841 gcatttgtaa taacaaatgg tttacagaaa cttcaatcat tctcttcctt aacaagaaag
      901 acctttttga ggaaaaaata aagaggagtc cgttaactat ctgttatcca gaatacacag
      961 gttccaatac atatgaagag gcagctgcct atattcaatg ccagtttgaa gatctgaaca
     1021 gaagaaaaga taccaaggag atctatactc acttcacctg tgccacagac acgaagaatg
     1081 tgcagtttgt ttttgatgct gttacagatg tcatcattaa aaacaactta aaggaatgtg
     1141 gactttattg a
//