LOCUS       AK303027                1207 bp    mRNA    linear   HUM 24-JUL-2008
DEFINITION  Homo sapiens cDNA FLJ59261 complete cds, highly similar to Mothers
            against decapentaplegic homolog 4 (SMAD4).
ACCESSION   AK303027
VERSION     AK303027.1
KEYWORDS    FLI_CDNA; oligo capping.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
            Chiyoda-ku, Tokyo 101-0044, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
            Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
            Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
            Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
  TITLE     NEDO human cDNA sequencing project focused on splicing variants
  JOURNAL   Unpublished (2007)
COMMENT     Human cDNA sequencing project focused on splicing variants of mRNA
            in NEDO functional analysis of protein and research application
            project supported by Ministry of Economy, Trade and Industry,
            Japan; cDNA selection for complete cds sequencing: Reverse
            Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
            (Hitachi) and Japan Biological Informatics Consortium, Japan
            (JBIC); cDNA complete cds sequencing: JBIC; cDNA library
            construction: Helix Research Institute supported by Japan Key
            Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
            Research Association for Biotechnology, Japan, Biotechnology
            Center, National Institute of Technology and Evaluation, Japan and
            HRI; cDNA mapping to human genome: Central Research Laboratory,
            Hitachi; evaluation and annotation: REPRORI.
FEATURES             Location/Qualifiers
     source          1..1207
                     /clone="TESTI4049958"
                     /clone_lib="TESTI4"
                     /db_xref="H-InvDB:HIT000497651"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /organism="Homo sapiens"
                     /tissue_type="testis"
     CDS             368..901
                     /codon_start=1
                     /note="highly similar to Mothers against decapentaplegic
                     homolog 4 (SMAD4)"
                     /protein_id="BAG64153.1"
                     /transl_table=1
                     /translation="MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLV
                     KKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWP
                     DLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNENNWYFSQL
                     LLDLDKGMIQDQAIHGP"
BASE COUNT          357 a          267 c          280 g          303 t
ORIGIN      
        1 gtagccccgc ggacagctgg gccagggtgc gggcctgcgc ctccctcgcc tcctggcgcg
       61 ggcctcgggg agaggggtgg aagatgtcta tggatgtgac attcctgggg acgggtgcag
      121 catacccatc tccaacccgg ggtgcctctg ctgtggtcct tcggtgtgaa ggcgagtgct
      181 ggctctttga ctgtggggag ggaacacaga cacagcttat gaaaagccaa cttaaagcag
      241 gttatcctga atacatgtct aacaattttc cttgcaacgt tagctgttgt ttttcactgt
      301 ttccaaagga tcaaaattgc ttcagaaatt ggagacatat ttgatttaaa aggaaaaact
      361 tgaacaaatg gacaatatgt ctattacgaa tacaccaaca agtaatgatg cctgtctgag
      421 cattgtgcat agtttgatgt gccatagaca aggtggagag agtgaaacat ttgcaaaaag
      481 agcaattgaa agtttggtaa agaagctgaa ggagaaaaaa gatgaattgg attctttaat
      541 aacagctata actacaaatg gagctcatcc tagtaaatgt gttaccatac agagaacatt
      601 ggatgggagg cttcaggtgg ctggtcggaa aggatttcct catgtgatct atgcccgtct
      661 ctggaggtgg cctgatcttc acaaaaatga actaaaacat gttaaatatt gtcagtatgc
      721 gtttgactta aaatgtgata gtgtctgtgt gaatccatat cactacgaac gagttgtatc
      781 acctggaatt gatctctcag gattaacact gcagagtaat gaaaacaact ggtatttctc
      841 acagctcctc ctagatttgg acaaaggaat gatacaagac caggcaatac atggtccata
      901 aatcaaaata ctataaaaag gtatacagtg aaaagaagtg ctcccacccc tgcccccatt
      961 cccccctacc tcagctccat caagtatgat ggtgaaggat gaatatgtgc atgactttga
     1021 gggacagcca tcgttgtcca ctgaaggaca ttcaattcaa accatccagc atccaccaag
     1081 taatcgtgca tcgacagaga catacagcac cccagctctg ttagccccat ctgagtctaa
     1141 tgctaccagc actgccaact ttcccaacat tcctgtggct tccacaagtc agcctgccag
     1201 tatactg
//