LOCUS       AK301165                1015 bp    mRNA    linear   HUM 31-JUL-2008
DEFINITION  Homo sapiens cDNA FLJ57208 complete cds, highly similar to
            5'-AMP-activated protein kinase subunit beta-1.
ACCESSION   AK301165
VERSION     AK301165.1
KEYWORDS    FLI_CDNA; oligo capping.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
            Chiyoda-ku, Tokyo 101-0044, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
            Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
            Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
            Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
  TITLE     NEDO human cDNA sequencing project focused on splicing variants
  JOURNAL   Unpublished (2007)
COMMENT     Human cDNA sequencing project focused on splicing variants of mRNA
            in NEDO functional analysis of protein and research application
            project supported by Ministry of Economy, Trade and Industry,
            Japan; cDNA selection for complete cds sequencing: Reverse
            Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
            (Hitachi) and Japan Biological Informatics Consortium, Japan
            (JBIC); cDNA complete cds sequencing: JBIC; cDNA library
            construction: Helix Research Institute supported by Japan Key
            Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
            Research Association for Biotechnology, Japan, Biotechnology
            Center, National Institute of Technology and Evaluation, Japan and
            HRI; cDNA mapping to human genome: Central Research Laboratory,
            Hitachi; evaluation and annotation: REPRORI.
FEATURES             Location/Qualifiers
     source          1..1015
                     /clone="SPLEN2021725"
                     /clone_lib="SPLEN2"
                     /db_xref="H-InvDB:HIT000495789"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /organism="Homo sapiens"
                     /tissue_type="spleen"
     CDS             250..951
                     /codon_start=1
                     /note="highly similar to 5'-AMP-activated protein kinase
                     subunit beta-1"
                     /protein_id="BAG62754.1"
                     /transl_table=1
                     /translation="MDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTV
                     FRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSE
                     PIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPE
                     ERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATH
                     RYKKKYVTTLLYKPI"
BASE COUNT          224 a          314 c          265 g          212 t
ORIGIN      
        1 aatcttcgcg ccccttgcgt tcttgccgcg gcttgcctgg gcaggtaaag cgcgattgcg
       61 agagctcggc aaccctgccg actcagccgg aaccggctcc cggcccgagg ggcgtggtgt
      121 cctggtgctc cgactccttc cgcaggctcc ttgggacccg cggttccggg agtcccttgc
      181 tcagggtccc tttcctgcag tgaggcgccg tccgccttcc ctgtgtcccc gcagaccccc
      241 aagatcctga tggacagccc cgaagacgcc gacctcttcc actccgagga aatcaaggca
      301 ccagagaagg aggaattcct ggcctggcag catgatctgg aagtgaatga taaagctccc
      361 gcccaggctc ggccaacggt gtttcgatgg acggggggcg gaaaggaagt ttacttatct
      421 gggtccttca acaactggag taaacttccc ctcaccagaa gccacaataa ctttgtagcc
      481 atcctggatc tgccggaagg agagcatcag tacaagttct ttgtggatgg tcagtggacg
      541 cacgaccctt ccgagcccat agtaaccagc cagcttggca cagttaacaa catcattcaa
      601 gtgaagaaaa ctgactttga agtatttgat gctttaatgg tggattccca aaagtgctcc
      661 gatgtgtctg agctgtccag ttctccccca ggaccctacc atcaggagcc ctacgtctgc
      721 aaacccgaag agcgctttcg ggcaccccct attctccccc cacatctcct ccaggtcatc
      781 ctgaacaagg acacggggat ttcctgtgat ccagctttgc ttcctgagcc caatcacgtc
      841 atgctgaacc acctatacgc gctgtctatc aaggatggag tgatggtgct cagcgcaacc
      901 caccggtaca agaagaagta cgtcaccacc ttgttataca agcccatatg aagagctggg
      961 ggcggatggt ggcccaggag acagcacacc accaggctcc acacgtgcat gcttt
//