LOCUS       AK300734                1588 bp    mRNA    linear   HUM 31-JUL-2008
DEFINITION  Homo sapiens cDNA FLJ60165 complete cds, highly similar to
            Translation initiation factor eIF-2B subunit delta.
ACCESSION   AK300734
VERSION     AK300734.1
KEYWORDS    FLI_CDNA; oligo capping.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
            Chiyoda-ku, Tokyo 101-0044, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
            Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
            Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
            Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
  TITLE     NEDO human cDNA sequencing project focused on splicing variants
  JOURNAL   Unpublished (2007)
COMMENT     Human cDNA sequencing project focused on splicing variants of mRNA
            in NEDO functional analysis of protein and research application
            project supported by Ministry of Economy, Trade and Industry,
            Japan; cDNA selection for complete cds sequencing: Reverse
            Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
            (Hitachi) and Japan Biological Informatics Consortium, Japan
            (JBIC); cDNA complete cds sequencing: JBIC; cDNA library
            construction: Helix Research Institute supported by Japan Key
            Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
            Research Association for Biotechnology, Japan, Biotechnology
            Center, National Institute of Technology and Evaluation, Japan and
            HRI; cDNA mapping to human genome: Central Research Laboratory,
            Hitachi; evaluation and annotation: REPRORI.
FEATURES             Location/Qualifiers
     source          1..1588
                     /cell_line="SK-N-MC"
                     /cell_type="neuroepithelioma"
                     /clone="SKNMC1000111"
                     /clone_lib="SKNMC1"
                     /db_xref="H-InvDB:HIT000495358"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /organism="Homo sapiens"
     CDS             239..1177
                     /codon_start=1
                     /note="highly similar to Translation initiation factor
                     eIF-2B subunit delta"
                     /protein_id="BAG62407.1"
                     /transl_table=1
                     /translation="MKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPE
                     TGSAVSAAQCQGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQAR
                     KGEQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSK
                     VSLFSHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQ
                     QVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKR
                     EEEVMSMRNKEGMHLWVKLRNMLKTPGKANVSLFLP"
BASE COUNT          402 a          409 c          454 g          323 t
ORIGIN      
        1 ctaggactga gggcgatggc tgctgtggcc gtggctgttc gcgagggtga gtgaagagcc
       61 gggcctgcct actggtacgc ggagccgacg agctccggac agctagtgcc gggccttgag
      121 cgcctttggg ccccgccgct ccgttccagc gccacgctgg gctccggtct acacagtctc
      181 gagcgcagtc ccgcgccgcg caccggctgg ttgctgtgag gaggactcgg gatccgggat
      241 gaaggcggag cttccccctg ggcctggggc agtggggagg gaaatgacca aagaagaaaa
      301 gctgcagctt cggaaggaaa agaaacagca gaagaagaaa cggaaggaag aaaagggggc
      361 agaaccagag actggctctg ctgtatctgc agcccaatgt caaggcccaa ccagagaact
      421 gccagaatcg ggcattcagt tgggcactcc tcgggagaaa gttccagctg gtcggagtaa
      481 ggccgaactt cgggctgagc gtcgagccaa gcaggaggcc gagcgggccc tgaaacaggc
      541 aagaaaaggg gaacaaggag gaccacctcc taaggccagc cccagcacag ctggagaaac
      601 cccctcagga gtgaagcgtc tccctgagta ccctcaggtt gatgacctac ttctgagaag
      661 gcttgttaaa aaaccagagc gtcaacaggt tcctacacga aaggattatg gatccaaagt
      721 cagtctcttc tctcacctac cccagtacag cagacaaaac tctctgaccc agtttatgag
      781 catcccatcc tctgtgatcc acccagccat ggtgcgactc ggcctgcagt actcccaggg
      841 cctggtcagt ggctccaatg cccggtgtat tgccctgctt cgtgccttgc agcaggtgat
      901 tcaggattac acaacaccac ctaatgaaga actctccagg gatctagtga ataaactaaa
      961 accctacatg agcttcctga ctcagtgccg tcccctgtca gcgagcatgc acaacgccat
     1021 caagttcctt aacaaggaaa tcaccagtgt gggcagttcc aagcgggaag aggaggtgat
     1081 gagtatgaga aataaggaag gcatgcacct gtgggtaaaa ttaagaaaca tgttaaaaac
     1141 tcccgggaag gccaacgtaa gccttttttt gccctagtca agggttaagg aatggtttgg
     1201 ccattctgac ttgtgccatc tctgagccct ctataggctt gtcatcatac acatattcaa
     1261 gcacctggca tgatcatcag agggataaaa gtgtccattt cttgttcact aaggatttta
     1321 ctccatttag gccaagtcag aacttcgagc agccattgat cggtatgtgc aagagaagat
     1381 tgtgctagca gctcaggcaa tttcacgctt tgcttaccag aagatcagta atggagatgt
     1441 gatcctggta tatggatgct catctctggt atcacgaatt cttcaggagg cttggacaga
     1501 gggccggcgg tttcgggtgg tagtggtgga cagccggcca tggctggaag gaaggcacac
     1561 actacgttct ctagtccatg ctggtgtc
//