LOCUS       AK298996                1050 bp    mRNA    linear   HUM 31-JUL-2008
DEFINITION  Homo sapiens cDNA FLJ58927 complete cds, highly similar to
            Endoplasmin precursor (Heat shock protein 90 kDa beta member 1).
ACCESSION   AK298996
VERSION     AK298996.1
KEYWORDS    FLI_CDNA; oligo capping.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
            Chiyoda-ku, Tokyo 101-0044, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
            Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
            Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
            Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
  TITLE     NEDO human cDNA sequencing project focused on splicing variants
  JOURNAL   Unpublished (2007)
COMMENT     Human cDNA sequencing project focused on splicing variants of mRNA
            in NEDO functional analysis of protein and research application
            project supported by Ministry of Economy, Trade and Industry,
            Japan; cDNA selection for complete cds sequencing: Reverse
            Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
            (Hitachi) and Japan Biological Informatics Consortium, Japan
            (JBIC); cDNA complete cds sequencing: JBIC; cDNA library
            construction: Helix Research Institute supported by Japan Key
            Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
            Research Association for Biotechnology, Japan, Biotechnology
            Center, National Institute of Technology and Evaluation, Japan and
            HRI; cDNA mapping to human genome: Central Research Laboratory,
            Hitachi; evaluation and annotation: REPRORI.
FEATURES             Location/Qualifiers
     source          1..1050
                     /cell_line="NT2"
                     /cell_type="teratocarcinoma"
                     /clone="NT2RI2022817"
                     /clone_lib="NT2RI2"
                     /db_xref="H-InvDB:HIT000493620"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /note="mRNA from NT2 neuronal precursor cells treated
                     2-weeks mitotic inhibitor after 5-weeks retinoic acid
                     (RA) induction."
                     /note="majorly NT2 neuron"
                     /organism="Homo sapiens"
     CDS             107..598
                     /codon_start=1
                     /note="highly similar to Endoplasmin precursor (Heat
                     shock protein 90 kDa beta member 1)"
                     /protein_id="BAG61085.1"
                     /transl_table=1
                     /translation="MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRT
                     DDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLR
                     ELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKKHLITLNWIQLKISSKNI
                     HSS"
BASE COUNT          360 a          173 c          271 g          246 t
ORIGIN      
        1 agtgggcgga ccgcgcggct ggaggtgtga ggatccgaac ccaggggtgg ggggtggagg
       61 cggctcctgc gatcgaaggg gacttgagac tcaccggccg cacgccatga gggccctgtg
      121 ggtgctgggc ctctgctgcg tcctgctgac cttcgggtcg gtcagagctg acgatgaagt
      181 tgatgtggat ggtacagtag aagaggatct gggtaaaagt agagaaggat caaggacgga
      241 tgatgaagta gtacagagag aggaagaagc tattcagttg gatggattaa atgcatcaca
      301 aataagagaa cttagagaga agtcggaaaa gtttgccttc caagccgaag ttaacagaat
      361 gatgaaactt atcatcaatt cattgtataa aaataaagag attttcctga gagaactgat
      421 ttcaaatgct tctgatgctt tagataagat aaggctaata tcactgactg atgaaaatgc
      481 tctttctgga aatgaggaac taacagtcaa aattaagtgt gataaggaga agaagcatct
      541 gattaccttg aattggatac aattaaaaat ctcgtcaaaa aatattcaca gttcataaac
      601 tttcctattt atgtatggag cagcaagact gaaactgttg aggagcccat ggaggaagaa
      661 gaagcagcca aagaagagaa agaagaatct gatgatgaag ctgcagtaga ggaagaagaa
      721 gaagaaaaga aaccaaagac taaaaaagtt gaaaaaactg tctggggctg ggaacttatg
      781 aatgatatca aaccaatatg gcagagacca tcaaaagaag tagaagaaga tgaatacaaa
      841 gctttctaca aatcattttc aaaggaaagt gatgacccca tggcttatat tcactttact
      901 gctgaagggg aagttacctt caaatcaatt ttatttgtac ccacatctgc tccacgtggt
      961 ctgtttgacg aatatggatc taaaaagagc gattacatta agctctatgt gcgccgtgta
     1021 ttcatcacag acgacttcca tgatatgatg
//