LOCUS       AK297937                1655 bp    mRNA    linear   HUM 31-JUL-2008
DEFINITION  Homo sapiens cDNA FLJ59358 complete cds, highly similar to
            Tissue-type plasminogen activator precursor (EC 3.4.21.68).
ACCESSION   AK297937
VERSION     AK297937.1
KEYWORDS    FLI_CDNA; oligo capping.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
            Chiyoda-ku, Tokyo 101-0044, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
            Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
            Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
            Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
  TITLE     NEDO human cDNA sequencing project focused on splicing variants
  JOURNAL   Unpublished (2007)
COMMENT     Human cDNA sequencing project focused on splicing variants of mRNA
            in NEDO functional analysis of protein and research application
            project supported by Ministry of Economy, Trade and Industry,
            Japan; cDNA selection for complete cds sequencing: Reverse
            Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
            (Hitachi) and Japan Biological Informatics Consortium, Japan
            (JBIC); cDNA complete cds sequencing: JBIC; cDNA library
            construction: Helix Research Institute supported by Japan Key
            Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
            Research Association for Biotechnology, Japan, Biotechnology
            Center, National Institute of Technology and Evaluation, Japan and
            HRI; cDNA mapping to human genome: Central Research Laboratory,
            Hitachi; evaluation and annotation: REPRORI.
FEATURES             Location/Qualifiers
     source          1..1655
                     /cell_type="dermal papilla cells (HDPC)"
                     /clone="HHDPC2002201"
                     /clone_lib="HHDPC2"
                     /db_xref="H-InvDB:HIT000492561"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /note="primary culture, dermal papilla cells"
                     /organism="Homo sapiens"
     CDS             99..1409
                     /codon_start=1
                     /note="highly similar to Tissue-type plasminogen
                     activator precursor (EC 3.4.21.68)"
                     /protein_id="BAG60253.1"
                     /transl_table=1
                     /translation="MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARSYQVICRDE
                     KTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKRNSDCYFGNGSAYRGTHS
                     LTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRL
                     TWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGG
                     ILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDT
                     YDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYS
                     ERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLV
                     CLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP"
BASE COUNT          391 a          460 c          470 g          334 t
ORIGIN      
        1 agagctgaga tcctacagga gtccagggct ggagagaaaa cctctgcgag gaaagggaag
       61 gagcaagccg tgaatttaag ggacgctgtg aagcaatcat ggatgcaatg aagagagggc
      121 tctgctgtgt gctgctgctg tgtggagcag tcttcgtttc gcccagccag gaaatccatg
      181 cccgattcag aagaggagcc agatcttacc aagtgatctg cagagatgaa aaaacgcaga
      241 tgatatacca gcaacatcag tcatggctgc gccctgtgct cagaagcaac cgggtggaat
      301 attgctggtg caacagtggc agggcacagt gccactcagt gcctgtcaaa agaaacagtg
      361 actgctactt tgggaatggg tcagcctacc gtggcacgca cagcctcacc gagtcgggtg
      421 cctcctgcct cccgtggaat tccatgatcc tgataggcaa ggtttacaca gcacagaacc
      481 ccagtgccca ggcactgggc ctgggcaaac ataattactg ccggaatcct gatggggatg
      541 ccaagccctg gtgccacgtg ctgaagaacc gcaggctgac gtgggagtac tgtgatgtgc
      601 cctcctgctc cacctgcggc ctgagacagt acagccagcc tcagtttcgc atcaaaggag
      661 ggctcttcgc cgacatcgcc tcccacccct ggcaggctgc catctttgcc aagcacagga
      721 ggtcgcccgg agagcggttc ctgtgcgggg gcatactcat cagctcctgc tggattctct
      781 ctgccgccca ctgcttccag gagaggtttc cgccccacca cctgacggtg atcttgggca
      841 gaacataccg ggtggtccct ggcgaggagg agcagaaatt tgaagtcgaa aaatacattg
      901 tccataagga attcgatgat gacacttacg acaatgacat tgcgctgctg cagctgaaat
      961 cggattcgtc ccgctgtgcc caggagagca gcgtggtccg cactgtgtgc cttcccccgg
     1021 cggacctgca gctgccggac tggacggagt gtgagctctc cggctacggc aagcatgagg
     1081 ccttgtctcc tttctattcg gagcggctga aggaggctca tgtcagactg tacccatcca
     1141 gccgctgcac atcacaacat ttacttaaca gaacagtcac cgacaacatg ctgtgtgctg
     1201 gagacactcg gagcggcggg ccccaggcaa atttgcacga cgcctgccag ggcgattcgg
     1261 gaggccccct ggtgtgtctg aacgatggtc gcatgacttt ggtgggcatc atcagctggg
     1321 gcctgggctg tggacagaag gatgtcccgg gtgtgtacac caaggttacc aactacctag
     1381 actggattcg tgacaacatg cgaccgtgac caggaacacc cgactcctca aaagcaaatg
     1441 agatcccgcc tcttcttctt cagaagacac tgcaaaggcg cagtgcttct ctacagactt
     1501 ctccagaccc accacaccgc agaagcggga cgagacccta caggagaggg aagagtgcat
     1561 tttcccagat acttcccatt ttggaagttt tcaggacttg gtctgatttc aggatactct
     1621 gtcagatggg aagacatgaa tgcacactag cctct
//