LOCUS       AK297764                1393 bp    mRNA    linear   HUM 24-JUL-2008
DEFINITION  Homo sapiens cDNA FLJ51901 complete cds, highly similar to Gap
            junction alpha-1 protein.
ACCESSION   AK297764
VERSION     AK297764.1
KEYWORDS    FLI_CDNA; oligo capping.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
            Chiyoda-ku, Tokyo 101-0044, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
            Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
            Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
            Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
  TITLE     NEDO human cDNA sequencing project focused on splicing variants
  JOURNAL   Unpublished (2007)
COMMENT     Human cDNA sequencing project focused on splicing variants of mRNA
            in NEDO functional analysis of protein and research application
            project supported by Ministry of Economy, Trade and Industry,
            Japan; cDNA selection for complete cds sequencing: Reverse
            Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
            (Hitachi) and Japan Biological Informatics Consortium, Japan
            (JBIC); cDNA complete cds sequencing: JBIC; cDNA library
            construction: Helix Research Institute supported by Japan Key
            Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
            Research Association for Biotechnology, Japan, Biotechnology
            Center, National Institute of Technology and Evaluation, Japan and
            HRI; cDNA mapping to human genome: Central Research Laboratory,
            Hitachi; evaluation and annotation: REPRORI.
FEATURES             Location/Qualifiers
     source          1..1393
                     /cell_type="chondrocytes (HC)"
                     /clone="HCHON2001157"
                     /clone_lib="HCHON2"
                     /db_xref="H-InvDB:HIT000492388"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /note="primary culture, chondrocytes"
                     /organism="Homo sapiens"
     CDS             205..1305
                     /codon_start=1
                     /note="highly similar to Gap junction alpha-1 protein"
                     /protein_id="BAG60112.1"
                     /transl_table=1
                     /translation="MGDWSALGKLLDKVQAYSTAGGKVWLSAWGDEQSAFRCNTQQPG
                     CENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQT
                     DGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYI
                     YGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFK
                     GVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGD
                     RNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAG
                     HELQPLAIVDQRPSSRASSRASSRPRPDDLEI"
BASE COUNT          373 a          324 c          336 g          360 t
ORIGIN      
        1 aaaaagcttt tacgaggtat cagcactttt ctttcattag ggggaaggcg tgaggaaagt
       61 accaaacagc agcggagttt taaactttaa atagacaggt ctgagtgcct gaacttgcct
      121 tttcatttta cttcatcctc caaggagttc aatcacttgg cgtgacttca ctacttttaa
      181 gcaaaagagt ggtgcccagg caacatgggt gactggagcg ccttaggcaa actccttgac
      241 aaggttcaag cctactcaac tgctggaggg aaggtgtggc tgtcagcctg gggagatgag
      301 cagtctgcct ttcgttgtaa cactcagcaa cctggttgtg aaaatgtctg ctatgacaag
      361 tctttcccaa tctctcatgt gcgcttctgg gtcctgcaga tcatatttgt gtctgtaccc
      421 acactcttgt acctggctca tgtgttctat gtgatgcgaa aggaagagaa actgaacaag
      481 aaagaggaag aactcaaggt tgcccaaact gatggtgtca atgtggacat gcacttgaag
      541 cagattgaga taaagaagtt caagtacggt attgaagagc atggtaaggt gaaaatgcga
      601 ggggggttgc tgcgaaccta catcatcagt atcctcttca agtctatctt tgaggtggcc
      661 ttcttgctga tccagtggta catctatgga ttcagcttga gtgctgttta cacttgcaaa
      721 agagatccct gcccacatca ggtggactgt ttcctctctc gccccacgga gaaaaccatc
      781 ttcatcatct tcatgctggt ggtgtccttg gtgtccctgg ccttgaatat cattgaactc
      841 ttctatgttt tcttcaaggg cgttaaggat cgggttaagg gaaagagcga cccttaccat
      901 gcgaccagtg gtgcgctgag ccctgccaaa gactgtgggt ctcaaaaata tgcttatttc
      961 aatggctgct cctcaccaac cgctcccctc tcgcctatgt ctcctcctgg gtacaagctg
     1021 gttactggcg acagaaacaa ttcttcttgc cgcaattaca acaagcaagc aagtgagcaa
     1081 aactgggcta attacagtgc agaacaaaat cgaatggggc aggcgggaag caccatctct
     1141 aactcccatg cacagccttt tgatttcccc gatgataacc agaattctaa aaaactagct
     1201 gctggacatg aattacagcc actagccatt gtggaccagc gaccttcaag cagagccagc
     1261 agtcgtgcca gcagcagacc tcggcctgat gacctggaga tctagataca ggcttgaaag
     1321 catcaagatt ccactcaatt gtggagaaga aaaaaggtgc tgtagaaagt gcaccaggtg
     1381 ttaattttga tcc
//