LOCUS       AK297103                1585 bp    mRNA    linear   HUM 31-JUL-2008
DEFINITION  Homo sapiens cDNA FLJ57320 complete cds, highly similar to
            Dihydrolipoyllysine-residue acetyltransferase component of
            pyruvate dehydrogenase complex, mitochondrial precursor (EC
            2.3.1.12).
ACCESSION   AK297103
VERSION     AK297103.1
KEYWORDS    FLI_CDNA; oligo capping.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
            Chiyoda-ku, Tokyo 101-0044, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
            Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
            Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
            Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
  TITLE     NEDO human cDNA sequencing project focused on splicing variants
  JOURNAL   Unpublished (2007)
COMMENT     Human cDNA sequencing project focused on splicing variants of mRNA
            in NEDO functional analysis of protein and research application
            project supported by Ministry of Economy, Trade and Industry,
            Japan; cDNA selection for complete cds sequencing: Reverse
            Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
            (Hitachi) and Japan Biological Informatics Consortium, Japan
            (JBIC); cDNA complete cds sequencing: JBIC; cDNA library
            construction: Helix Research Institute supported by Japan Key
            Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
            Research Association for Biotechnology, Japan, Biotechnology
            Center, National Institute of Technology and Evaluation, Japan and
            HRI; cDNA mapping to human genome: Central Research Laboratory,
            Hitachi; evaluation and annotation: REPRORI.
FEATURES             Location/Qualifiers
     source          1..1585
                     /cell_type="normal dermal fibroblasts (Neonatal Skin)
                     (NHDF2564)"
                     /clone="DFNES2002575"
                     /clone_lib="DFNES2"
                     /db_xref="H-InvDB:HIT000491727"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /note="primary culture, normal dermal fibroblasts"
                     /organism="Homo sapiens"
     CDS             63..1349
                     /codon_start=1
                     /note="highly similar to Dihydrolipoyllysine-residue
                     acetyltransferase component of pyruvate dehydrogenase
                     complex, mitochondrial precursor (EC 2.3.1.12)"
                     /protein_id="BAG59614.1"
                     /transl_table=1
                     /translation="MWRVCARRAQNVAPWAGLEARWTALQEVPGTPRVTSRSGPAPVR
                     RNSVTTGYGGVRALCGWTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPT
                     MQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAVEKGIDLTQVKGTG
                     PDGRITKKDIDSFVPSKVAPAPAAVVPPTGPGMAPVSTGVFTDIPISNIRRVIAQRLM
                     QSKQTIPHYYLSINVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEAN
                     SSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKL
                     QPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASM
                     MSVTLSCDHRVVDGAVGAQWLAEFRKYLEKPITMLL"
BASE COUNT          425 a          333 c          387 g          440 t
ORIGIN      
        1 gctcttggag aggtcactcc ggagacggcg ttggttttgg ggtgtggggg gttggtggca
       61 ctatgtggcg cgtctgtgcg cgacgggctc agaatgtagc cccatgggcg ggactcgagg
      121 ctcggtggac ggccttgcag gaggtacccg gaactccacg agtgacctcg cgatctggcc
      181 cggctcccgt tcgtcgcaac agcgtgacta cagggtatgg cggggtccgg gcactgtgcg
      241 gctggacccc cagttctggg gccacgccgc ggaaccgctt actgctgcag cttttggggt
      301 cgcccggccg ccgctattac agtcttcccc cgcatcagaa ggttccattg ccttctcttt
      361 cccccacaat gcaggcaggc accatagccc gttgggaaaa aaaagagggg gacaaaatca
      421 atgaaggtga cctaattgca gaggtcgaaa ctgataaagc cactgttgga tttgagagcc
      481 tggaggagtg ttatatggca gtagagaaag ggattgatct tacacaagta aaagggacag
      541 gaccagatgg tagaatcacc aagaaggata tcgactcttt tgtgcctagt aaagttgctc
      601 ctgctccggc agctgttgtg cctcccacag gtcctggaat ggcaccagtt tctacaggtg
      661 tcttcacaga tatcccaatc agcaacattc gtcgggttat tgcacagcga ttaatgcaat
      721 caaagcaaac catacctcat tattaccttt ctatcaatgt aaatatggga gaagttttgt
      781 tggtacggaa agaacttaat aagatattag aagggagaag caaaatttct gtcaatgact
      841 tcatcataaa agcttcagct ttggcatgtt taaaagttcc cgaagcaaat tcttcttgga
      901 tggacacagt tataagacaa aatcatgttg ttgatgtcag tgttgcggtc agtactcctg
      961 caggactcat cacacctatt gtgtttaatg cacatataaa aggagtggaa accattgcta
     1021 atgatgttgt ttctttagca accaaagcaa gagagggtaa actacagcca catgaattcc
     1081 agggtggcac ttttacgatc tccaatttag gaatgtttgg aattaagaat ttctctgcta
     1141 ttattaaccc acctcaagca tgtattttgg caattggtgc ttcagaggat aaactggtcc
     1201 ctgcagataa tgaaaaaggg tttgatgtgg ctagcatgat gtctgttaca ctcagttgtg
     1261 atcaccgggt ggtggatgga gcagttggag cccagtggct tgctgagttt agaaagtacc
     1321 ttgaaaaacc tatcactatg ttgttgtaac taactcaaga atttctaaac tctcccaggt
     1381 cacactgatt cattcttaac aagatattta tatgttatta aacaggtggt tctttttatt
     1441 ttaaccagtt atttttatta ttgagtctgt ccagataagt tatttataat gggcattact
     1501 gaatttttaa aatgccgatt acacccaaat attgtgcaca tttaataatc agacaccaga
     1561 tttttagctc tgtactccta attaa
//