LOCUS       AK131521                2431 bp    mRNA    linear   HUM 09-JAN-2008
DEFINITION  Homo sapiens cDNA FLJ41041 fis, clone NT2RI2004312.
ACCESSION   AK131521
VERSION     AK131521.1
KEYWORDS    FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Isogai,T. and Yamamoto,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2004) to the DDBJ/EMBL/GenBank databases.
            Contact:Takao Isogai
            Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
            Kisarazu, Chiba 292-0812, Japan
            E-mail :flj-cdna@nifty.com
REFERENCE   2  
  AUTHORS   Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
            Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
            Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
            Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
            Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
            Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
            Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
            Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
            Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
  TITLE     NEDO human cDNA sequencing project
  JOURNAL   Unpublished (2004)
COMMENT     NEDO human cDNA sequencing project supported by Ministry of
            Economy, Trade and Industry of Japan; cDNA full insert sequencing:
            Research Association for Biotechnology (RAB); cDNA library
            construction: Helix Research Institute (HRI) (supported by Japan
            Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
            RAB, HRI, and Biotechnology Center, National Institute of
            Technology and Evaluation; clone selection for full insert
            sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES             Location/Qualifiers
     source          1..2431
                     /cell_line="NT2"
                     /cell_type="teratocarcinoma"
                     /clone="NT2RI2004312"
                     /clone_lib="NT2RI2"
                     /db_xref="H-InvDB:HIT000249809"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="cloning vector: pME18SFL3"
                     /note="mRNA from NT2 neuronal precursor cells treated
                     2-weeks mitotic inhibitor after 5-weeks retinoic acid
                     (RA) induction."
                     /note="majorly NT2 neuron"
                     /organism="Homo sapiens"
BASE COUNT          595 a          562 c          596 g          678 t
ORIGIN      
        1 acctgcacaa ggcgcgcgac ggcccttgcg agttcgctcc tgtggtcgtc gttcctcccc
       61 gaagtgttca caacgtcacc ggggcgcagg tgggcctgtc ctgtgaagtg agggctgtgc
      121 ctaccccagt catcacgtgg agaaaggtca cgaagtcccc tgagggcacc caagcactgg
      181 aggagctgcc tggggaccat gtcaatatag ctgtccaagt gcgagggggc ccttctgacc
      241 atgaggccac ggcctggatt ttgatcaacc ccctgcgaaa ggaggatgag ggtgtgtacc
      301 agtgccatgc agccaacatg gtgggagagg ctgagtccca cagcacagtg acggttctag
      361 atctgagtaa atacaggagc ttccacttcc cagctcccga tgaccgcatg tgatggagaa
      421 atggtgttag aaacattgat catgggatga tggaaaagtc aaataacgga tctttgtgct
      481 tcatgaagag ttggaaaacc tgtgtgtgta gatgacccct tttgtgtgtt tttaaaaatt
      541 agatgcaaac tagatttgta tgcagatgta gtttttagca gggcaaacag tgagaaacgg
      601 atttgcatgt ggctttttta tacttttgaa atgaattgtt ccatgagaag tctttttgta
      661 attactctct tccagggaga tcacagaatg gcatgtttgc aatttcgaaa gggctcgtgt
      721 cagctgtgac tctttacctc atttgtctat gacctattag tggtgctctg tgacttagtg
      781 tgtgttgtag aaggagggat gagggtccca gctgtcctct gcctgactgt aacttgctgt
      841 gtgacctgaa caggtgcctt cccctctctg ggccctggtc ccttcatctg tgagtatggc
      901 attttaactg gatgttatca atgtcccttt tagccctgcc atcctagagt aagatggcct
      961 tttgggaaaa taacaggaag tgtggtctta gtgttctttc taaaatggta ttccctcaga
     1021 aacaaattcc attgctttac cccagcgtgt ggacagctgg ctgtagctct tctattccta
     1081 actttttccc tttgaaggag atttagtttt aggtgggatg ctttaccaga ctcaggagcg
     1141 ccttggctgg gtctttggag accagagcat ccatttgtta gcctagcatt ctctttttta
     1201 aaattttatt ttgagacagg gtctcgctct atcacccagg ctggagtgca gtggcgtgat
     1261 catggctcac tgcaacctcc actttctggg ctcgagtgat ccttccacct cagggtcatc
     1321 tcaaaactat gtaaaggcag atgttgccaa tccacaggct tcagggactc ttatcaagac
     1381 tcatcccaac ttttaaggca cactttcaca ccgctcacat tgtgcattac attttggagt
     1441 ccttgttgga agtgaacttt ccgcctgtaa gcctgccatt ccatgtctaa aatattggga
     1501 tggcatcacc ttctgtacta tgcagcagaa ggagactggc ctgggattct ggaaacatga
     1561 gatccatttg catattaatc cccggctcgc tgtgtgaccc tgagcaagtc tccttcactt
     1621 tctgtgcctc agtgtcctta tttgagtaaa aagggaataa aactagatga gtggttttca
     1681 aactttaaaa cactggaagc tctctttgaa aacaaaagat gagaattgaa atggatggca
     1741 ctggaagctg ttcttgtcta ctggggtttg gagctgccag gggacctgct cacatccact
     1801 cctcactcag cccctcccgc ccctcccttc tccacgcact gtgactgact tccatgtgtg
     1861 agagcctgca gttaattctc ctgtgtcttg aatggttggg agatgagttg gtcagaccct
     1921 tagtgaaatg atgtgggaag gaacaggaat gctgtggctc tgaagaaggt agtagaacat
     1981 cccacacctg ctaataagca cattttgcaa actcagttga ctcacctcag atttgcctag
     2041 tgaaaactga agggctggat gtagacccag agagcagggt ggacagagcc catgctggaa
     2101 gtactgagca tcaggactgt atggggctgg ctttagcatc atcattctgt gcaaagacaa
     2161 ataaataatt aatgtcacac tgtctgaaat tttcaccaaa accaaaaagg cgcattaaca
     2221 tgattgttcc aacctcatgg tttttcaagt gtgagcgccc agagacctct tcagaggcta
     2281 agagagcatg cgctgccaca gctgttagca tttggagacg taattacaat gcccccactc
     2341 cccgcaccaa acctgggttt ttctaccgtt tttgtagatg tcatgatgtc acgacccctg
     2401 caccctgaaa aaaataaatg tttttaagga c
//