LOCUS X02910 3634 bp DNA linear HUM 14-NOV-2006
DEFINITION Human gene for tumor necrosis factor (TNF-alpha).
ACCESSION X02910 X02159
VERSION X02910.1
KEYWORDS signal peptide; tumor necrosis factor.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3634)
AUTHORS Nedwin G.E., Naylor S.L., Sakaguchi A.Y., Smith D.,
Jarrett-Nedwin J., Pennica D., Goeddel D.V., Gray P.W.
TITLE Human lymphotoxin and tumor necrosis factor genes: structure,
homology and chromosomal localization
JOURNAL Nucleic Acids Res. 13(17), 6361-6373(1985).
PUBMED 2995927
REFERENCE 2 (bases 329 to 3634)
AUTHORS Shirai T., Yamaguchi H., Ito H., Todd C.W., Wallace R.B.
TITLE Cloning and expression in Escherichia coli of the gene for human
tumour necrosis factor
JOURNAL Nature 313(6005), 803-806(1985).
PUBMED 3883195
REFERENCE 3
AUTHORS Pennica D., Nedwin G.E., Hayflick J.S., Seeburg P.H., Derynck R.,
Palladino M.A., Kohr W.J., Aggarwal B.B., Goeddel D.V.
TITLE Human tumour necrosis factor: precursor structure, expression and
homology to lymphotoxin
JOURNAL Nature 312(5996), 724-729(1984).
PUBMED 6392892
COMMENT In the cDNA sequence from ref [3] the mature protein site starts
also with Val at pos 1631
Data kindly reviewed (18-FEB-1986) by A. Sakaguchi
FEATURES Location/Qualifiers
source 1..3634
/organism="Homo sapiens"
/mol_type="genomic DNA"
/db_xref="taxon:9606"
regulatory 590..595
/regulatory_class="TATA_box"
prim_transcript 615..3381
/note="put. primary transcript"
exon 615..981
/number=1
misc_difference 768
/replace="c"
/note="conflict"
/citation=[3]
CDS join(796..981,1589..1634,1822..1869,2171..2592)
/product="TNF-alpha"
/db_xref="GOA:P01375"
/db_xref="HGNC:HGNC:11892"
/db_xref="InterPro:IPR002959"
/db_xref="InterPro:IPR006052"
/db_xref="InterPro:IPR006053"
/db_xref="InterPro:IPR008983"
/db_xref="InterPro:IPR021184"
/db_xref="PDB:1A8M"
/db_xref="PDB:1TNF"
/db_xref="PDB:2AZ5"
/db_xref="PDB:2E7A"
/db_xref="PDB:2TUN"
/db_xref="PDB:2ZJC"
/db_xref="PDB:2ZPX"
/db_xref="PDB:3ALQ"
/db_xref="PDB:3IT8"
/db_xref="PDB:3L9J"
/db_xref="PDB:3WD5"
/db_xref="PDB:4G3Y"
/db_xref="PDB:4TSV"
/db_xref="PDB:4TWT"
/db_xref="PDB:4Y6O"
/db_xref="PDB:5M2I"
/db_xref="PDB:5M2J"
/db_xref="PDB:5M2M"
/db_xref="PDB:5MU8"
/db_xref="PDB:5TSW"
/db_xref="PDB:5UUI"
/db_xref="PDB:5WUX"
/db_xref="PDB:5YOY"
/db_xref="UniProtKB/Swiss-Prot:P01375"
/protein_id="CAA26669.1"
/translation="MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGA
TTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQL
QWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIA
VSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYL
DFAESGQVYFGIIAL"
sig_peptide join(796..981,1589..1630)
/note="put. signal peptide (aa -76 to -1; aa -78 to -3 in
ref.[2])"
intron 982..1588
/number=1
misc_difference 1092
/replace=""
/note="conflict"
/citation=[2]
/citation=[3]
exon 1589..1634
/number=2
mat_peptide join(1631..1634,1822..1869,2171..2589)
/product="TNF-alpha"
intron 1635..1821
/number=2
exon 1822..1869
/number=3
misc_feature 1822..1823
/note="put. signal peptide (aa -1, ref. [2])"
misc_feature 1824..1869
/note="TNF-alpha (aa 1-15, ref. 2)"
intron 1870..2170
/number=3
exon 2171..3381
/number=4
misc_feature 2171..2589
/note="TNF-alpha (aa 18-157; aa 16-155 in ref. [2])"
misc_difference 2791
/replace="t"
/note="conflict"
/citation=[3]
misc_difference 2882
/replace="a"
/note="conflict"
/citation=[2]
misc_difference 3326
/replace="a"
/note="conflict"
/citation=[2]
regulatory 3368..3373
/regulatory_class="polyA_signal_sequence"
polyA_site 3381
BASE COUNT 906 a 959 c 975 g 794 t
ORIGIN
1 gaattccggg tgatttcact cccggctgtc caggcttgtc ctgctacccc acccagcctt
61 tcctgaggcc tcaagcctgc caccaagccc ccagctcctt ctccccgcag gacccaaaca
121 caggcctcag gactcaacac agcttttccc tccaacccgt tttctctccc tcaacggact
181 cagctttctg aagcccctcc cagttctagt tctatctttt tcctgcatcc tgtctggaag
241 ttagaaggaa acagaccaca gacctggtcc ccaaaagaaa tggaggcaat aggttttgag
301 gggcatgggg acggggttca gcctccaggg tcctacacac aaatcagtca gtggcccaga
361 agacccccct cggaatcgga gcagggagga tggggagtgt gaggggtatc cttgatgctt
421 gtgtgtcccc aactttccaa atccccgccc ccgcgatgga gaagaaaccg agacagaagg
481 tgcagggccc actaccgctt cctccagatg agctcatggg tttctccacc aaggaagttt
541 tccgctggtt gaatgattct ttccccgccc tcctctcgcc ccagggacat ataaaggcag
601 ttgttggcac acccagccag cagacgctcc ctcagcaagg acagcagagg accagctaag
661 agggagagaa gcaactacag accccccctg aaaacaaccc tcagacgcca catcccctga
721 caagctgcca ggcaggttct cttcctctca catactgacc cacggcttca ccctctctcc
781 cctggaaagg acaccatgag cactgaaagc atgatccggg acgtggagct ggccgaggag
841 gcgctcccca agaagacagg ggggccccag ggctccaggc ggtgcttgtt cctcagcctc
901 ttctccttcc tgatcgtggc aggcgccacc acgctcttct gcctgctgca ctttggagtg
961 atcggccccc agagggaaga ggtgagtgcc tggccagcct tcatccactc tcccacccaa
1021 ggggaaatga gagacgcaag agagggagag agatgggatg ggtgaaagat gtgcgctgat
1081 agggagggat gagagagaaa aaaacatgga gaaagacggg gatgcagaaa gagatgtggc
1141 aagagatggg gaagagagag agagaaagat ggagagacag gatgtctggc acatggaagg
1201 tgctcactaa gtgtgtatgg agtgaatgaa tgaatgaatg aatgaacaag cagatatata
1261 aataagatat ggagacagat gtggggtgtg agaagagaga tgggggaaga aacaagtgat
1321 atgaataaag atggtgagac agaaagagcg ggaaatatga cagctaagga gagagatggg
1381 ggagataagg agagaagaag atagggtgtc tggcacacag aagacactca gggaaagagc
1441 tgttgaatgc tggaaggtga atacacagat gaatggagag agaaaaccag acacctcagg
1501 gctaagagcg caggccagac aggcagccag ctgttcctcc tttaagggtg actccctcga
1561 tgttaaccat tctccttctc cccaacagtt ccccagggac ctctctctaa tcagccctct
1621 ggcccaggca gtcagtaagt gtctccaaac ctctttccta attctgggtt tgggtttggg
1681 ggtagggtta gtaccggtat ggaagcagtg ggggaaattt aaagttttgg tcttggggga
1741 ggatggatgg aggtgaaagt aggggggtat tttctaggaa gtttaagggt ctcagctttt
1801 tcttttctct ctcctcttca ggatcatctt ctcgaacccc gagtgacaag cctgtagccc
1861 atgttgtagg taagagctct gaggatgtgt cttggaactt ggagggctag gatttgggga
1921 ttgaagcccg gctgatggta ggcagaactt ggagacaatg tgagaaggac tcgctgagct
1981 caagggaagg gtggaggaac agcacaggcc ttagtgggat actcagaacg tcatggccag
2041 gtgggatgtg ggatgacaga cagagaggac aggaaccgga tgtggggtgg gcagagctcg
2101 agggccagga tgtggagagt gaaccgacat ggccacactg actctcctct ccctctctcc
2161 ctccctccag caaaccctca agctgagggg cagctccagt ggctgaaccg ccgggccaat
2221 gccctcctgg ccaatggcgt ggagctgaga gataaccagc tggtggtgcc atcagagggc
2281 ctgtacctca tctactccca ggtcctcttc aagggccaag gctgcccctc cacccatgtg
2341 ctcctcaccc acaccatcag ccgcatcgcc gtctcctacc agaccaaggt caacctcctc
2401 tctgccatca agagcccctg ccagagggag accccagagg gggctgaggc caagccctgg
2461 tatgagccca tctatctggg aggggtcttc cagctggaga agggtgaccg actcagcgct
2521 gagatcaatc ggcccgacta tctcgacttt gccgagtctg ggcaggtcta ctttgggatc
2581 attgccctgt gaggaggacg aacatccaac cttcccaaac gcctcccctg ccccaatccc
2641 tttattaccc cctccttcag acaccctcaa cctcttctgg ctcaaaaaga gaattggggg
2701 cttagggtcg gaacccaagc ttagaacttt aagcaacaag accaccactt cgaaacctgg
2761 gattcaggaa tgtgtggcct gcacagtgaa gtgctggcaa ccactaagaa ttcaaactgg
2821 ggcctccaga actcactggg gcctacagct ttgatccctg acatctggaa tctggagacc
2881 agggagcctt tggttctggc cagaatgctg caggacttga gaagacctca cctagaaatt
2941 gacacaagtg gaccttaggc cttcctctct ccagatgttt ccagacttcc ttgagacacg
3001 gagcccagcc ctccccatgg agccagctcc ctctatttat gtttgcactt gtgattattt
3061 attatttatt tattatttat ttatttacag atgaatgtat ttatttggga gaccggggta
3121 tcctggggga cccaatgtag gagctgcctt ggctcagaca tgttttccgt gaaaacggag
3181 ctgaacaata ggctgttccc atgtagcccc ctggcctctg tgccttcttt tgattatgtt
3241 ttttaaaata tttatctgat taagttgtct aaacaatgct gatttggtga ccaactgtca
3301 ctcattgctg agcctctgct ccccagggga gttgtgtctg taatcgccct actattcagt
3361 ggcgagaaat aaagtttgct tagaaaagaa acatggtctc cttcttggaa ttaattctgc
3421 atctgcctct tcttgtgggt gggaagaagc tccctaagtc ctctctccac aggctttaag
3481 atccctcgga cccagtccca tccttagact cctagggccc tggagaccct acataaacaa
3541 agcccaacag aatattcccc atcccccagg aaacaagagc ctgaacctaa ttacctctcc
3601 ctcagggcat gggaatttcc aactctggga attc
//