LOCUS W41487 1122 bp mRNA linear EST 11-SEP-1996
DEFINITION mc42d10.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone
IMAGE:351187 5' similar to gb:J00413 Mouse beta-globin major gene
(MOUSE), mRNA sequence.
ACCESSION W41487
VERSION W41487.1
DBLINK BioSample: SAMN00155042
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 1122)
AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
Waterston,R.
TITLE The WashU-HHMI Mouse EST Project
JOURNAL Unpublished
COMMENT Contact: Marra M/Mouse EST Project
WashU-HHMI Mouse EST Project
Washington University School of MedicineP
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: mouseest@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
MGI:222987
Seq primer: ETPrimer
High quality sequence stop: 542.
FEATURES Location/Qualifiers
source 1..1122
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/clone="IMAGE:351187"
/clone_lib="SAMN00155042 Soares mouse p3NMF19.5"
/dev_stage="19.5 dpc total fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Vector: pT7T3D (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 5. Library constructed by Bento
Soares and M.Fatima Bonaldo. RNA was kindly provided by
Dr. Minoru Ko (Wayne State University)."
BASE COUNT 256 a 215 c 364 g 267 t
ORIGIN
1 catatggaag tgtcgtgcag cgaaaggtag cntgtgtgtg tgtactgcac gaagcccgca
61 gtaatagcag tacagtcaat gtggtgcgac gcgtgactgg ggatgctgta gagatggtct
121 gcatgtactc gttnggccgt gtgtggcgaa tgagnggtga agcgcctgat tgggatagtt
181 gtgatgagtg aatggcccgt ggtgcaaggc ntngnnctgc attgtgctga cagcttggag
241 cccgagtcgg atagctgttg aggtagcgtt gtggaggacc gtatgcctca tgccgtcgtg
301 ctgatcgatg ngnngtaatg ccgaagnagt gagaggcctc aatggcatac gaatagtgag
361 taacgtgcgc tgttagacga aatggccgtg aatcacgtgt ggacgagccg tcagagnggc
421 gaccgtgttg ccgagcgctc tnagtgagcg tccacntgat gacgaagcnn tgcgatgtgg
481 atccgatgag aactgtcgag gctcgctggg cgaatgatga tcnngtgagt tgtgctgggc
541 caccaacctt gggcaagcga tttcacccgc cgcgtgcgac aggcntgcct tccgagaaag
601 gtggatggct ggaagtggcc ggatggcatg gcttcacaag taccactaag accccgtttc
661 atgctctatg cctgtgaacg aatggttaat tgtgtcgaag agacgacgnt gtcagttgtt
721 gcaaaatgat aaagacatgt gaaaacctgc atcctgacaa taaaacgatt tatttactcg
781 aaaaaaagta agaaaaaaat gcgcgcgacg ttattcctta agtggggtaa attacgttga
841 atggcggtcg tttaaatgtg tgattgagac cttggggtcc gagttactcg gtgagggacc
901 cgttctgagt ggtaatacgg aaagccgcca gatcgtcgac agttccgggg aatgggaatg
961 accgctttct cgggttgagc gcgggttggt ctgcgaaagt aaatgtagcg cgggcgtttg
1021 ttcttttttt ggaggggctt tggagagtaa agcgctatga ggggtgatcc tcacaagtgt
1081 gcccgaagct tctgccggta agttgagaca caaggtttta gt
//