LOCUS W41451 428 bp mRNA linear EST 12-SEP-1996 DEFINITION mc47a03.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:351628 5' similar to gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence. ACCESSION W41451 VERSION W41451.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 428) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:223428 Seq primer: -28M13 rev2 from Amersham High quality sequence stop: 379. FEATURES Location/Qualifiers source 1..428 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:351628" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." BASE COUNT 91 a 110 c 123 g 104 t ORIGIN 1 gttgtgttga cttgcaacct cagaaacaga catcatggtg cacctgactg atgctgagaa 61 ggctgctgtc tctggcctgt ggggaaaggt gaacgccgat gaagttggtg gtgaggccct 121 gggcaggctg ctggttgtct acccttggac ccagcggtac tttgatagct ttggagacct 181 atcctctgcc tctgctatca tgggtaatgc caaagtgaag gcccatggca agaaagtgat 241 aactgccttt aacgatggcc tgaatcactt ggacagcctc aagggcacct ttgccagcct 301 cagtgagctc cactgtgaca agctgcatgt ggatcctgag aacttcaggc tcctggggca 361 atatgatcgt gattgtgctg ggccaccacc tgggccaaga tttcaccccg gctgcacagg 421 ctgctttc //