LOCUS       W41161                   471 bp    mRNA    linear   EST 12-SEP-1996
DEFINITION  mc42d01.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone
            IMAGE:351169 5' similar to gb:V00497 HEMOGLOBIN BETA CHAIN (HUMAN);
            gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence.
ACCESSION   W41161
VERSION     W41161.1
DBLINK      BioSample: SAMN00155042
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:222969
            Trace considered overall poor quality
            Seq primer: -28M13 rev2 from Amersham
            Sequence considered overall poor quality.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:351169"
                     /clone_lib="SAMN00155042 Soares mouse p3NMF19.5"
                     /dev_stage="19.5 dpc total fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. RNA was kindly provided by
                     Dr. Minoru Ko (Wayne State University)."
BASE COUNT          112 a          104 c          146 g          109 t
ORIGIN      
        1 tgactggcaa cctcagacac agacatcatg gtgcacctga ctgatgctga gaaggctgct
       61 gtctctggcc tgtggagaaa ggtgaacgcc gatgaagtgg gtggtgaggc cctgggcagg
      121 ctgctgggtg tggatcctgg gaggcatcgg tacgttgata gctatggaga cctatcctct
      181 gactctgcta tcatgggtaa tgccaaagtg aaggtacatg ggaagaaagt gataactgcc
      241 gattaacgat gtgctgaagc acttggacag catcaatgtc acgtttgcca gcatcagtga
      301 gctcgactgt gacaggctgc atgtgaatac tgagaacttc aggctcctgg gcaatatgat
      361 cgtgattgtc ctgagcaaca gttgggcaag gatatgagcg cgctgcacag gctgttgcca
      421 gaatgtggtg tctgcagtcg ttgctgcctg gctcacaagt acactaagcc c
//