LOCUS W41145 490 bp mRNA linear EST 11-SEP-1996 DEFINITION mc39a10.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:350874 5' similar to gb:V00497 HEMOGLOBIN BETA CHAIN (HUMAN); gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence. ACCESSION W41145 VERSION W41145.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 490) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:222674 Putative full length read Seq primer: ETPrimer High quality sequence stop: 219. FEATURES Location/Qualifiers source 1..490 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:350874" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." BASE COUNT 109 a 127 c 131 g 123 t ORIGIN 1 ctcacaaccc cagaaacaga catcatggtg cacctgactg atgctgagaa ggctgctgtc 61 tctggcctgt ggggaaaggt gaacgccgat gaagttggtg gtgaggccct gggcaggctg 121 ctggttgtct acccttggac ccagcggtac tttgatagct ttggagacct atcctctgcc 181 tctgctatca tgggtaatgc caaagtgaag gcccatggaa agaaagtgat aactgccttt 241 aacgatggcc tgaatcactt ggacagcctc aagggcacct ttgcaagcct cagtgagctc 301 cactgtgaca agcgtcatgt ggatcctgag aactgtcagc tcctgggcat tatgatcgtg 361 atttgtgctg gcccacacct gggcaaggat ttcacccggc ttgaaagctt gcttccaata 421 ggttgtgctt gaattgccgc tgcctggctc caaagtccat tgaaaccctt tcctgttttg 481 ccctgaacaa //